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Invitrogen
Doublecortin Recombinant Rabbit Monoclonal Antibody (JJ0959)
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FIGURE: 1 / 4
Doublecortin Antibody (MA5-32495) in WB
Western blot was performed using Anti-Doublecortin Monoclonal Antibody(Product # MA5-32495) and 40 kDa band corresponding to Doublecortin was observed across the tissues tested except Mouse, Rat Liver and Mouse, Rat Spleen. Tissue extracts (30 µg lysate) of Mouse Brain (Lane 1), Rat Brain (Lane 2), Mouse Cerebellum (Lane 3), Mouse Liver (Lane 4), Rat Liver (Lane 5), Mouse Spleen (Lane 6) and Rat Spleen (Lane 7) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto ... View More
Product Details
MA5-32495
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JJ0959
Immunogen
Synthetic peptide within Human Doublecortin aa 22-71
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2809772
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. DCX is a cytoplasmic protein which appears to direct neuronal migration by regulating the organization and stability of microtubules. It contains two doublecortin domains, which bind microtubules. In addition, this protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in the gene encoding DCX are a cause of X-linked lissencephaly.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: doublecortex; Doublin; Lis-X; LIS-XD; Lissencephalin-X; lissencephaly, X-linked (doublecortin); Neuronal migration protein doublecortin; OTTHUMP00000023860; OTTHUMP00000216316
Gene Aliases: DBCN; Dbct; DC; Dcn; DCX; LISX; SCLH; XLIS
UniProt ID: (Human) A9Z1V8, (Mouse) O88809
Entrez Gene ID: (Human) 1641, (Rat) 84394, (Mouse) 13193
neuron migration
brain development
central nervous system projection neuron axonogenesis
intracellular signal transduction
axon extension
dendrite morphogenesis
response to electrical stimulus
multicellular organism development
nervous system development
peptidyl-serine phosphorylation
cell differentiation
protein autophosphorylation
central nervous system development
hippocampus development
layer formation in cerebral cortex
pyramidal neuron development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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