Invitrogen

Dynamin 1 Polyclonal Antibody

Dynamin 1 Antibody in Immunocytochemistry (ICC/IF)

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Dynamin 1 Antibody (PA1-660) in ICC/IF

Immunofluorescence analysis of Dynamin I was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Dynamin I Rabbit Polyclonal Antibody (Product # PA1-660) at 2µg/mL in 0.1% BSA and incubated for 3 hours at ro... View More

Dynamin 1 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

Dynamin 1 Antibody in Western Blot, Immunoprecipitation (WB, IP)

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Product Details

PA1-660

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

View 14 publications 14 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

Assay-dependent

View 5 publications 5 publications

Immunoprecipitation (IP)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic Peptide corresponding to resdiues C K(633) E K A S E T E E N G S D S F(647) of rat Dyn1.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_325845

Product Specific Information

PA1-660 detects dynamin I (Dyn1) from rat tissues.

PA1-660 has been successfully used in Western blot and immunocytochemical procedures. By Western blot, this antibody detects an ~100 kDa protein representing Dyn1 from rat whole brain extract.

PA1-660 immunizing peptide corresponds to amino acid residues 633-647 from rat Dyn1. This sequence is completely conserved between rat, mouse, and human Dyn1. PA1-660 immunizing peptide (Cat. # PEP-042) is available for use in neutralization and control experiments.

Target Information

Dynamin is a GTPase required for endocytosis of clathrin coated vesicles from the membrane. Dynamin's involvement in endocytosis was first discovered during the characterization of the temperature sensitive parylytic shibire mutation from Drosophila melanogaster. Three closely related isoforms have been identified in mammals. Dynamin1 is expressed exclusively in neurons, dynamin2 is ubiquitously expressed and dynamin3 is expressed primarily in the testes. Each of these isoforms of dynamin also has several splice variants. This suggests that each isoform/splice variant may have separate intracellular targets or function in the budding of distinct vesicle types. Common domain features of the dynamin proteins include an amino acid terminal GTPase domain, a central pleckstrin homology domain and a highly basic proline rich carboxyl terminus with several Src homology 3 (SH3) domains. These SH3 domains target the dynamin to the clathrin coated pit. Dynamin is known to interact with at least two proteins, AP2 adaptor protein complex and amphiphysin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Translational potential of synaptic alterations in Alzheimer's disease patients and amyloid precursor protein knock-in mice.

Brain communications

Medina-Vera D,Enache D,Tambaro S,Abuhashish E,Rosell-Valle C,Winblad B,Rodríguez de Fonseca F,Bereczki E,Nilsson P

PA1-660 was used in Immunocytochemistry, Western Blot, Immunohistochemistry to find a significant increase in concentrations of zinc transporter protein 3 and AMPA glutamate receptor 3 in cerebrospinal fluid of both patient groups compared with cognitively healthy controls.

Wed Feb 01 00:00:00 UTC 2023

Dopaminergic neurodegeneration induced by Parkinson's disease-linked G2019S LRRK2 is dependent on kinase and GTPase activity.

Proceedings of the National Academy of Sciences of the United States of America

Nguyen APT,Tsika E,Kelly K,Levine N,Chen X,West AB,Boularand S,Barneoud P,Moore DJ

Published figure using Dynamin 1 polyclonal antibody (Product # PA1-660) in Western Blot

Tue Jul 21 00:00:00 UTC 2020

Dopaminergic neurodegeneration induced by Parkinson's disease-linked G2019S LRRK2 is dependent on kinase and GTPase activity.

Proceedings of the National Academy of Sciences of the United States of America

Nguyen APT,Tsika E,Kelly K,Levine N,Chen X,West AB,Boularand S,Barneoud P,Moore DJ

Published figure using Dynamin 1 polyclonal antibody (Product # PA1-660) in Western Blot

Tue Jul 21 00:00:00 UTC 2020

Dynamin 1 is important for microtubule organization and stabilization in glomerular podocytes.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

La TM,Tachibana H,Li SA,Abe T,Seiriki S,Nagaoka H,Takashima E,Takeda T,Ogawa D,Makino SI,Asanuma K,Watanabe M,Tian X,Ishibe S,Sakane A,Sasaki T,Wada J,Takei K,Yamada H

PA1-660 was used in Immunocytochemistry to suggest that dynamin 1 supports the microtubule bundle formation and participates in the stabilization of microtubules.

Tue Dec 01 00:00:00 UTC 2020

Dynamin 1 Restrains Vesicular Release to a Subquantal Mode In Mammalian Adrenal Chromaffin Cells.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Wu Q,Zhang Q,Liu B,Li Y,Wu X,Kuo S,Zheng L,Wang C,Zhu F,Zhou Z

PA1-660 was used in Western Blotting to provide compelling evidence that most Ca2+-induced secretory events occur via the subquantal mode in native adrenal chromaffin cells.

Wed Jan 09 00:00:00 UTC 2019

JC Polyomavirus Entry by Clathrin-Mediated Endocytosis Is Driven by β-Arrestin.

Journal of virology

Mayberry CL,Soucy AN,Lajoie CR,DuShane JK,Maginnis MS

PA1-660 was used in Western Blotting to characterize how JCPyV utilizes the clathrin-mediated endocytosis pathway to invade host cells.

Mon Apr 15 00:00:00 UTC 2019

The invasive proteome of glioblastoma revealed by laser-capture microdissection.

Neuro-oncology advances

Daubon T,Guyon J,Raymond AA,Dartigues B,Rudewicz J,Ezzoukhry Z,Dupuy JW,Herbert JMJ,Saltel F,Bjerkvig R,Nikolski M,Bikfalvi A

PA1-660 was used in Immunohistochemistry to perform a proteomics analysis of laser-capture micro-dissected glioblastoma core and invasive areas of patient-derived xenografts.

Fri Apr 15 00:00:00 UTC 2022

The invasive proteome of glioblastoma revealed by laser-capture microdissection.

Neuro-oncology advances

Daubon T,Guyon J,Raymond AA,Dartigues B,Rudewicz J,Ezzoukhry Z,Dupuy JW,Herbert JMJ,Saltel F,Bjerkvig R,Nikolski M,Bikfalvi A

PA1-660 was used in Immunohistochemistry to perform a proteomics analysis of laser-capture micro-dissected glioblastoma core and invasive areas of patient-derived xenografts.

Fri Apr 15 00:00:00 UTC 2022

The invasive proteome of glioblastoma revealed by laser-capture microdissection.

Neuro-oncology advances

Daubon T,Guyon J,Raymond AA,Dartigues B,Rudewicz J,Ezzoukhry Z,Dupuy JW,Herbert JMJ,Saltel F,Bjerkvig R,Nikolski M,Bikfalvi A

PA1-660 was used in Immunohistochemistry to perform a proteomics analysis of laser-capture micro-dissected glioblastoma core and invasive areas of patient-derived xenografts.

Fri Apr 15 00:00:00 UTC 2022

PICK1 regulates AMPA receptor endocytosis via direct interactions with AP2 α-appendage and dynamin.

The Journal of cell biology

Fiuza M,Rostosky CM,Parkinson GT,Bygrave AM,Halemani N,Baptista M,Milosevic I,Hanley JG

Published figure using Dynamin 1 polyclonal antibody (Product # PA1-660) in Western Blot

Mon Oct 02 00:00:00 UTC 2017

Dynamin 1 isoform roles in a mouse model of severe childhood epileptic encephalopathy.

Neurobiology of disease

Asinof S,Mahaffey C,Beyer B,Frankel WN,Boumil R

PA1-660 was used in immunoprecipitation and western blot to characterize the role of dynamin 1 isoform using a severe epileptic encephalopathy mouse model

Tue Nov 01 00:00:00 UTC 2016

Dynamin 1 isoform roles in a mouse model of severe childhood epileptic encephalopathy.

Neurobiology of disease

Asinof S,Mahaffey C,Beyer B,Frankel WN,Boumil R

PA1-660 was used in immunoprecipitation and western blot to characterize the role of dynamin 1 isoform using a severe epileptic encephalopathy mouse model

Tue Nov 01 00:00:00 UTC 2016

Actin bundling by dynamin 2 and cortactin is implicated in cell migration by stabilizing filopodia in human non-small cell lung carcinoma cells.

International journal of oncology

Yamada H,Takeda T,Michiue H,Abe T,Takei K

PA1-660 was used in immunocytochemistry elucidate the role of dynamin in the formation of actin-based membrane protrusions at the leading edge of cancer cells

Thu Sep 01 00:00:00 UTC 2016

Independent Neuronal Origin of Seizures and Behavioral Comorbidities in an Animal Model of a Severe Childhood Genetic Epileptic Encephalopathy.

PLoS genetics

Asinof SK,Sukoff Rizzo SJ,Buckley AR,Beyer BJ,Letts VA,Frankel WN,Boumil RM

PA1-660 was used in western blot to develop a conditional Dnm1Ftfl mouse model of epileptic encephalopathies.

Mon Jun 01 00:00:00 UTC 2015

Parkinson's disease-linked mutations in VPS35 induce dopaminergic neurodegeneration.

Human molecular genetics

Tsika E,Glauser L,Moser R,Fiser A,Daniel G,Sheerin UM,Lees A,Troncoso JC,Lewis PA,Bandopadhyay R,Schneider BL,Moore DJ

PA1-660 was used in western blot to study the induction of dopaminergic neurodegeneration by VPS35 mutations associated with Parkinson's disease

Mon Sep 01 00:00:00 UTC 2014

Dynamin 1 is required for memory formation.

PloS one

Fà M,Staniszewski A,Saeed F,Francis YI,Arancio O

PA1-660 was used in western blot to study the role of dynamin-1 in hippocampal-dependent associative memory

Mon Dec 29 00:00:00 UTC 2014

Synaptic function is modulated by LRRK2 and glutamate release is increased in cortical neurons of G2019S LRRK2 knock-in mice.

Frontiers in cellular neuroscience

Beccano-Kelly DA,Kuhlmann N,Tatarnikov I,Volta M,Munsie LN,Chou P,Cao LP,Han H,Tapia L,Farrer MJ,Milnerwood AJ

PA1-660 was used in western blot to determine the effect of the G2019S mutation of LRRK2 has on the synaptic release of mammalian neurons

Mon Oct 13 00:00:00 UTC 2014

Stabilization of actin bundles by a dynamin 1/cortactin ring complex is necessary for growth cone filopodia.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Yamada H,Abe T,Satoh A,Okazaki N,Tago S,Kobayashi K,Yoshida Y,Oda Y,Watanabe M,Tomizawa K,Matsui H,Takei K

PA1-660 was used in immunocytochemistry and western blot to demonstrate the role of a dynamin 1/cortactin ring complex for the maintenance of growth cone filopodia

Wed Mar 06 00:00:00 UTC 2013

Alzheimer brain-derived amyloid β-protein impairs synaptic remodeling and memory consolidation.

Neurobiology of aging

Borlikova GG,Trejo M,Mably AJ,Mc Donald JM,Sala Frigerio C,Regan CM,Murphy KJ,Masliah E,Walsh DM

PA1-660 was used in western blot to study the effect of water-soluble beta-amyloid extracts from Alzheimer's disease brains on synaptic remodeling and memory in recipient rats

Wed May 01 00:00:00 UTC 2013

Protein partners of dynamin-1 in the retina.

Visual neuroscience

Grossman GH,Ebke LA,Beight CD,Jang GF,Crabb JW,Hagstrom SA

PA1-660 was used in immunohistochemistry, immunoprecipitation, and western blot to identify protein binding partners of retinal dynamin-1

Mon Jul 01 00:00:00 UTC 2013

Protein partners of dynamin-1 in the retina.

Visual neuroscience

Grossman GH,Ebke LA,Beight CD,Jang GF,Crabb JW,Hagstrom SA

PA1-660 was used in immunohistochemistry, immunoprecipitation, and western blot to identify protein binding partners of retinal dynamin-1

Mon Jul 01 00:00:00 UTC 2013

Protein partners of dynamin-1 in the retina.

Visual neuroscience

Grossman GH,Ebke LA,Beight CD,Jang GF,Crabb JW,Hagstrom SA

PA1-660 was used in immunohistochemistry, immunoprecipitation, and western blot to identify protein binding partners of retinal dynamin-1

Mon Jul 01 00:00:00 UTC 2013

Protein partners of dynamin-1 in the retina.

Visual neuroscience

Grossman GH,Ebke LA,Beight CD,Jang GF,Crabb JW,Hagstrom SA

PA1-660 was used in immunohistochemistry, immunoprecipitation, and western blot to identify protein binding partners of retinal dynamin-1

Mon Jul 01 00:00:00 UTC 2013

Dynamin 1 depletion and memory deficits in rats treated with Abeta and cerebral ischemia.

Journal of neuroscience research

Watanabe T,Iwasaki K,Takasaki K,Yamagata N,Fujino M,Nogami A,Ii M,Katsurabayashi S,Mishima K,Fujiwara M

PA1-660 was used in western blot to investigate the mechanism of memory impairment induced by Abeta and cerebral ischemia

Thu Jul 01 00:00:00 UTC 2010

A missense mutation in a highly conserved alternate exon of dynamin-1 causes epilepsy in fitful mice.

PLoS genetics

Boumil RM,Letts VA,Roberts MC,Lenz C,Mahaffey CL,Zhang ZW,Moser T,Frankel WN

PA1-660 was used in western blot to study the role of a missense mutation in dynamin-1 in the pathogenesis of epilepsy in fitful mice

Thu Aug 05 00:00:00 UTC 2010

A missense mutation in a highly conserved alternate exon of dynamin-1 causes epilepsy in fitful mice.

PLoS genetics

Boumil RM,Letts VA,Roberts MC,Lenz C,Mahaffey CL,Zhang ZW,Moser T,Frankel WN

PA1-660 was used in western blot to study the role of a missense mutation in dynamin-1 in the pathogenesis of epilepsy in fitful mice

Thu Aug 05 00:00:00 UTC 2010

Endocytosis in cultured neurons is altered by chronic alcohol exposure.

Toxicological sciences : an official journal of the Society of Toxicology

Marín MP,Esteban-Pretel G,Ponsoda X,Romero AM,Ballestín R,López C,Megías L,Timoneda J,Molowny A,Canales JJ,Renau-Piqueras J

PA1-660 was used in western blot to investigate the influence of chronic alcohol consumption on neuronal endocytosis

Sat May 01 00:00:00 UTC 2010

Bioinformatics

Protein Aliases: B-dynamin; D100; Dyn1; Dynamin; Dynamin BREDNM19; Dynamin I; Dynamin, brain; Dynamin-1; Dynamin1; EC 3.6.5.5; Neuronal dynamin; OTTHUMP00000215500

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Gene Aliases: AI838169; DNM; DNM1; EIEE31; Ftfl; Kiaa4093; mKIAA4093

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UniProt ID: (Human) Q05193, (Mouse) P39053, (Rat) P21575

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Entrez Gene ID: (Human) 1759, (Mouse) 13429, (Rat) 140694

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Function(s)

GTPase activity protein binding GTP binding protein C-terminus binding protein kinase binding D2 dopamine receptor binding protein complex binding identical protein binding poly(A) RNA binding protein dimerization activity nitric-oxide synthase binding nucleotide binding hydrolase activity

Process(es)

G-protein coupled receptor internalization endocytosis receptor-mediated endocytosis endosome organization sensory perception of sound adult locomotory behavior ephrin receptor signaling pathway protein tetramerization synaptic transmission, GABAergic clathrin-mediated endocytosis toxin transport positive regulation of synaptic vesicle recycling receptor internalization metabolic process

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Dynamin 1 Polyclonal Antibody

Dynamin 1 Antibody (PA1-660) in ICC/IF

Product Details

PA1-660

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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