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Invitrogen
Dynamin 1 Monoclonal Antibody (A1A12)
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FIGURE: 1 / 4
Dynamin 1 Antibody (MA5-34957) in ICC/IF
Immunofluorescent analysis of Dynamin 1 in MG-63 cells (green). Samples were formalin fixed, permeabilized with 0.1% Triton X-100 in TBS (1 hour, room temperature) and blocked with 1% BSA (15 min, room temperature), incubated with Dynamin 1 monoclonal antibody (Product # MA5-34957) at a dilution of 1:50 (1 hour, room temperature), and followed by Alexa Fluor 488 Goat anti-Rabbit IgG and DAPI (blue) with a d... View More
Product Details
MA5-34957
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
A1A12
Immunogen
Recombinant protein within Human Dynamin 1 aa 555-770
Conjugate
Form
Liquid
Concentration
2 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 50% glycerol, 0.2% BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2848863
Product Specific Information
Positive Control: PC-3M cell lysates, MG-63, SiHa, A549.
Target Information
Dynamin is a GTPase required for endocytosis of clathrin coated vesicles from the membrane. Dynamin's involvement in endocytosis was first discovered during the characterization of the temperature sensitive parylytic shibire mutation from Drosophila melanogaster. Three closely related isoforms have been identified in mammals. Dynamin1 is expressed exclusively in neurons, dynamin2 is ubiquitously expressed and dynamin3 is expressed primarily in the testes. Each of these isoforms of dynamin also has several splice variants. This suggests that each isoform/splice variant may have separate intracellular targets or function in the budding of distinct vesicle types. Common domain features of the dynamin proteins include an amino acid terminal GTPase domain, a central pleckstrin homology domain and a highly basic proline rich carboxyl terminus with several Src homology 3 (SH3) domains. These SH3 domains target the dynamin to the clathrin coated pit. Dynamin is known to interact with at least two proteins, AP2 adaptor protein complex and amphiphysin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Dyn1; Dynamin; Dynamin BREDNM19; Dynamin I; Dynamin-1; Dynamin1; EC 3.6.5.5; Neuronal dynamin; OTTHUMP00000215500
Gene Aliases: DNM; DNM1; EIEE31
UniProt ID: (Human) Q05193
Entrez Gene ID: (Human) 1759
G-protein coupled receptor internalization
endocytosis
receptor-mediated endocytosis
endosome organization
sensory perception of sound
adult locomotory behavior
ephrin receptor signaling pathway
protein tetramerization
synaptic transmission, GABAergic
clathrin-mediated endocytosis
toxin transport
positive regulation of synaptic vesicle recycling
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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