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Invitrogen
Dynein Monoclonal Antibody (74.1), eBioscience™
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FIGURE: 1 / 3
Dynein Antibody (14-9772-80) in ICC/IF
Immunocytochemistry of methanol-fixed and permeabilized HeLa cells stained with 1 µg/mL of Mouse IgG2b K Isotype Control Purified (left) or 1 µg/mL of Anti-Dynein Purified (right) followed by F(ab')2 Anti-Mouse IgG eFluor® 570.Nuclei are stained with DAPI.
Product Details
14-9772-80
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Fruit fly,
Human,
Mouse,
Sheep,
Pig,
Rat,
Xenopus
Published species
Fruit fly,
Mouse
Host/Isotype
Mouse
/ IgG2b, kappa
Class
Monoclonal
Type
Antibody
Clone
74.1
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% Sodium Azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2573019
Product Specific Information
The monoclonal antibody 74.1 recognizes all known forms of the 74 kDa intermediate chain subunit of mammalian cytoplasmic dynein. Cytoplasmic dynein is a microtubule-stimulated ATPase responsible for movement of membrane-bound organelles in a directed manner toward the minus end of microtubules, commonly found at the centrosome. Dynein is responsible for the transport of lysosomes, endosomes, phagosomes, peroxisomes, mitochondria, and vesicles from the endoplasmic reticulum destined for the Golgi. During mitosis, dynein is responsible for positioning the spindle apparatus as well as being localized to the kinetochores to facilitate chromosome movement.The 74.1 antibody recognizes human, mouse, rat, bovine, sheep, Xenopus, porcine, and Drosophila dynein intermediate chain.
This 74.1 antibody has been tested by immunocytochemistry of methanol-fixed and permeabilized cells and can be used at less than or equal to 1 µg/mL. This 74.1 antibody has been tested by western blot of reduced and non-reduced HeLa cell lysate and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The directed movement of membrane-bounded organelles is an important process for various cellular functions including membrane transport, secretion, and axonal transport. Cytoplasmic dynein, a ubiquitous minus end-directed microtubule-based motor protein, is believed to be responsible for the retrograde transport of membranous organelles from the synapse to the cell body in fast axonal transport. The cytoplasmic dynein complex is composed of two ~530 kDa heavy chains, and intermediate chains of ~74 kDa and ~53-59 kDa.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 8 kDa dynein light chain; 8 kDa dynein light chain b; 8kD LC; 8kDa LC; cytoplasmic dynein light polypeptide; cytoplasmic light-chain dynein; cytosolic DH IC-1; DLC8; DLC8b; dynein LC8; Dynein light chain 1, cytoplasmic; Dynein light chain 2, cytoplasmic; Dynein light chain LC8-type 1; Dynein light chain LC8-type 2; dynein light chain-2; dynein, cytoplasmic, light chain 1; dynein, cytoplasmic, light peptide; dynein, cytoplasmic, light polypeptide 1; dynein, light chain, LC8-type 1; dynein, light chain, LC8-type 2; mPIN; PIN; Protein inhibitor of neuronal nitric oxide synthase; protein inhibitor of nitric oxide synthase; radial spoke 22 homolog
Gene Aliases: 1700064A15Rik; 6720463E02Rik; 8kDLC; BOS_16492; BOS_18355; C87222; CDLC2; DLC1; DLC2; DLC8; DLC8b; DNCL1; DNCL1B; DNCLC1; DYNLL1; DYNLL2; HDLC1; LC8; LC8a; PIN; RSPH22
transcription, DNA-templated
regulation of transcription, DNA-templated
nitric oxide biosynthetic process
transport
apoptotic process
microtubule-based process
substantia nigra development
neurotransmitter metabolic process
negative regulation of phosphorylation
negative regulation of catalytic activity
regulation of catalytic activity
ER to Golgi vesicle-mediated transport
synaptic target recognition
macroautophagy
antigen processing and presentation of exogenous peptide antigen via MHC class II
positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway
metabolic process
G2/M transition of mitotic cell cycle
female gamete generation
anatomical structure morphogenesis
viral process
actin cytoskeleton organization
intraciliary retrograde transport
cilium morphogenesis
microtubule-based movement
negative regulation of nitric oxide biosynthetic process
intracellular transport
modulation by virus of host morphology or physiology
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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