Invitrogen

Dystrophin Polyclonal Antibody

5 Published Figures

16 References

View all (36) Dystrophin antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (5)

Dystrophin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 6

Dystrophin Antibody (PA5-32388) in IHC (P)

Immunohistochemical (paraffin) analysis of Dystrophin using anti-Dystrophin Polyclonal Antibody (Product # PA5-32388) in Skeletal Muscle Cancer Tissue. The recommended dilution for this antibody in immunohistochemistry applications is 1:100.

Dystrophin Antibody in Immunocytochemistry (ICC/IF)

Dystrophin Antibody in Immunohistochemistry (Frozen) (IHC (F))

Dystrophin Antibody in Immunohistochemistry (IHC)

Dystrophin Antibody in Immunohistochemistry, Immunohistochemistry (PFA fixed) (IHC, IHC (PFA))

Dystrophin Antibody in Western Blot (WB)

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Product Details

PA5-32388

Applications

Tested Dilution

Publications

Western Blot (WB)

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1:400

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Bovine, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from C-terminus of human dystrophin.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. not determined

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.6, with 1% BSA

Contains

0.1% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2549858

Product Specific Information

This antibody is predicted to react with bovine, chicken, canine and porcine based on sequence homology.

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is skeletal muscle tissue.

Target Information

Dystrophin is the 427kDa protein product of the DMB/BMD gene located on the X chromosome at position Xp21. Western blotting and immunohistochemistry are the two established methods for the detection of abnormalities of dystrophin expression in muscle biopsies. Dystrophin abnormalities are thought to occur in 100% of patients with DMD/BMD, although genetic abnormalities may only be detected in up to 65% of cases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Intermittent glucocorticoid treatment enhances skeletal muscle performance through sexually dimorphic mechanisms.

The Journal of clinical investigation

Salamone IM,Quattrocelli M,Barefield DY,Page PG,Tahtah I,Hadhazy M,Tomar G,McNally EM

PA1-37587 was used in Immunohistochemistry to investigate the impact of once-weekly glucocorticoid exposure on skeletal muscle performance comparing male and female mice.

Tue Mar 15 00:00:00 UTC 2022

Intermittent glucocorticoid treatment enhances skeletal muscle performance through sexually dimorphic mechanisms.

The Journal of clinical investigation

Salamone IM,Quattrocelli M,Barefield DY,Page PG,Tahtah I,Hadhazy M,Tomar G,McNally EM

PA1-37587 was used in Immunohistochemistry to investigate the impact of once-weekly glucocorticoid exposure on skeletal muscle performance comparing male and female mice.

Tue Mar 15 00:00:00 UTC 2022

Skeletal muscle undergoes fiber type metabolic switch without myosin heavy chain switch in response to defective fatty acid oxidation.

Molecular metabolism

Pereyra AS,Lin CT,Sanchez DM,Laskin J,Spangenburg EE,Neufer PD,Fisher-Wellman K,Ellis JM

PA5-32388 was used in Immunohistochemistry (Frozen) to suggest that shifts in muscle catabolism are not sufficient to drive shifts in the contractile apparatus but are sufficient to drive adaptive changes in metabolic properties.

Sun May 01 00:00:00 UTC 2022

Skeletal muscle undergoes fiber type metabolic switch without myosin heavy chain switch in response to defective fatty acid oxidation.

Molecular metabolism

Pereyra AS,Lin CT,Sanchez DM,Laskin J,Spangenburg EE,Neufer PD,Fisher-Wellman K,Ellis JM

Sun May 01 00:00:00 UTC 2022

The adhesion G-protein-coupled receptor Gpr116 is essential to maintain the skeletal muscle stem cell pool.

Cell reports

Sénéchal C,Fujita R,Jamet S,Maiga A,Dort J,Orfi Z,Dumont NA,Bouvier M,Crist C

PA1-37587 was used in Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot to propose a model by which GPR116 maintains the MuSC pool via nuclear functions of ß-arrestin1.

Tue Nov 15 00:00:00 UTC 2022

Optogenetic modeling of human neuromuscular circuits in Duchenne muscular dystrophy with CRISPR and pharmacological corrections.

Science advances

Paredes-Redondo A,Harley P,Maniati E,Ryan D,Louzada S,Meng J,Kowala A,Fu B,Yang F,Liu P,Marino S,Pourquié O,Muntoni F,Wang J,Lieberam I,Lin YY

PA5-32388 was used in Western Blot to provide a new human microphysiological model for investigating neuromuscular junction defects in duchenne muscular dystrophy.

Fri Sep 10 00:00:00 UTC 2021

Optogenetic modeling of human neuromuscular circuits in Duchenne muscular dystrophy with CRISPR and pharmacological corrections.

Science advances

Paredes-Redondo A,Harley P,Maniati E,Ryan D,Louzada S,Meng J,Kowala A,Fu B,Yang F,Liu P,Marino S,Pourquié O,Muntoni F,Wang J,Lieberam I,Lin YY

PA5-32388 was used in Western Blot to provide a new human microphysiological model for investigating neuromuscular junction defects in duchenne muscular dystrophy.

Fri Sep 10 00:00:00 UTC 2021

LAT1 Protein Content Increases Following 12 Weeks of Resistance Exercise Training in Human Skeletal Muscle.

Frontiers in nutrition

Roberson PA,Mobley CB,Romero MA,Haun CT,Osburn SC,Mumford PW,Vann CG,Greer RA,Ferrando AA,Roberts MD

PA5-32388 was used in Immunohistochemistry to determine if amino acid transporters and amino acid metabolic enzymes were related to skeletal muscle hypertrophy following resistance exercise training with different nutritional supplementation strategies.

Wed Feb 03 00:00:00 UTC 2021

LAT1 Protein Content Increases Following 12 Weeks of Resistance Exercise Training in Human Skeletal Muscle.

Frontiers in nutrition

Roberson PA,Mobley CB,Romero MA,Haun CT,Osburn SC,Mumford PW,Vann CG,Greer RA,Ferrando AA,Roberts MD

Wed Feb 03 00:00:00 UTC 2021

Synergist ablation-induced hypertrophy occurs more rapidly in the plantaris than soleus muscle in rats due to different molecular mechanisms.

American journal of physiology. Regulatory, integrative and comparative physiology

Roberts MD,Mobley CB,Vann CG,Haun CT,Schoenfeld BJ,Young KC,Kavazis AN

PA5-32388 was used in Immunohistochemistry to examine molecular mechanisms that are altered during rapid soleus (type I fiber-dominant) and plantaris (type II fiber-dominant) hypertrophy in rats.

Sat Feb 01 00:00:00 UTC 2020

A gene-edited mouse model of limb-girdle muscular dystrophy 2C for testing exon skipping.

Disease models & mechanisms

Demonbreun AR,Wyatt EJ,Fallon KS,Oosterbaan CC,Page PG,Hadhazy M,Quattrocelli M,Barefield DY,McNally EM

Published figure using Dystrophin polyclonal antibody (Product # PA5-32388) in Immunohistochemistry

Mon Nov 04 00:00:00 UTC 2019

A gene-edited mouse model of limb-girdle muscular dystrophy 2C for testing exon skipping.

Disease models & mechanisms

Demonbreun AR,Wyatt EJ,Fallon KS,Oosterbaan CC,Page PG,Hadhazy M,Quattrocelli M,Barefield DY,McNally EM

Published figure using Dystrophin polyclonal antibody (Product # PA5-32388) in Immunohistochemistry

Mon Nov 04 00:00:00 UTC 2019

CRISPR/Cas9 Technology in Restoring Dystrophin Expression in iPSC-Derived Muscle Progenitors.

Journal of visualized experiments : JoVE

Jin Y,Shen Y,Su X,Weintraub N,Tang Y

PA5-32388 was used in Immunocytochemistry to show the feasibility of using CRISPR/Cas9 deletion strategy to restore dystrophin expression in iPSC-derived MPC, which has significant potential for developing future therapies for the treatment of DMD.

Sat Sep 14 00:00:00 UTC 2019

CRISPR/Cas9 Technology in Restoring Dystrophin Expression in iPSC-Derived Muscle Progenitors.

Journal of visualized experiments : JoVE

Jin Y,Shen Y,Su X,Weintraub N,Tang Y

Sat Sep 14 00:00:00 UTC 2019

Effect of Ibuprofen on Skeletal Muscle of Dysferlin-Null Mice.

The Journal of pharmacology and experimental therapeutics

Collier AF,Gumerson J,Lehtimäki K,Puoliväli J,Jones JW,Kane MA,Manne S,O'Neill A,Windish HP,Ahtoniemi T,Williams BA,Albrecht DE,Bloch RJ

PA5-32388 was used in Immunohistochemistry-immunofluorescence to indicate that ibuprofen can have deleterious effects on dysferlin-null muscle and suggest that its use at pharmacological doses should be avoided by individuals with dysferlinopathies.

Thu Mar 01 00:00:00 UTC 2018

Myoblots: dystrophin quantification by in-cell western assay for a streamlined development of Duchenne muscular dystrophy (DMD) treatments.

Neuropathology and applied neurobiology

Ruiz-Del-Yerro E,Garcia-Jimenez I,Mamchaoui K,Arechavala-Gomeza V

PA5-32388 was used in Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunocytochemistry to quantify dystrophin and other muscle-associated proteins in muscle cell cultures to distinguish accurately between the different sets of patients based on their dystrophin expression and detect dystrophin restoration after treatment.

Wed Aug 01 00:00:00 UTC 2018

Effect of extended postmortem aging and steak location on myofibrillar protein degradation and Warner-Bratzler shear force of beef M. semitendinosus steaks.

Journal of animal science

Phelps KJ,Drouillard JS,Silva MB,Miranda LD,Ebarb SM,Van Bibber-Krueger CL,O'Quinn TG,Gonzalez JM

PA1-37587 was used in immunohistochemistry - frozen section to study myofibrillar protein degradation and Warner-Bratzler shear force of beef M. semitendinosus steaks and the effect of extended postmortem aging and steak location

Fri Jan 01 00:00:00 UTC 2016

Direct reprogramming of urine-derived cells with inducible MyoD for modeling human muscle disease.

Skeletal muscle

Kim EY,Page P,Dellefave-Castillo LM,McNally EM,Wyatt EJ

PA1-37587 was used in immunocytochemistry discuss different cell sources from which to generate muscle cells

Mon Oct 23 00:00:00 UTC 2017

Phosphorylation of eIF2α Is a Translational Control Mechanism Regulating Muscle Stem Cell Quiescence and Self-Renewal.

Cell stem cell

Zismanov V,Chichkov V,Colangelo V,Jamet S,Wang S,Syme A,Koromilas AE,Crist C

PA1-37587 was used in immunohistochemistry study the role of eIF2alpha in skeletal muscle stem cells

Thu Jan 07 00:00:00 UTC 2016

Realimentation of nutrient restricted pregnant beef cows supports compensatory fetal muscle growth.

Journal of animal science

Gonzalez JM,Camacho LE,Ebarb SM,Swanson KC,Vonnahme KA,Stelzleni AM,Johnson SE

PA1-37587 was used in immunohistochemistry - frozen section to study the effect of nutrient restriction at specific gestational timeframes on fetal skeletal muscle growth

Tue Oct 01 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: Duchenne muscular dystrophy (DMD); Dystrophin; dystrophin Dp40; dystrophin Dp71a; dystrophin Dp71ab; dystrophin Dp71b; GS1-19O24.1; Muscular dystrophy Duchenne and Becker types; OTTHUMP00000215592; X-linked muscular dystrophy

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Gene Aliases: BMD; CMD3B; DMD; Dp427; Dp71; DXS142; DXS164; DXS206; DXS230; DXS239; DXS268; DXS269; DXS270; DXS272; DXSmh7; DXSmh9; dys; mdx; MRX85; pke

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UniProt ID: (Human) P11532, (Mouse) P11531

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Entrez Gene ID: (Human) 1756, (Mouse) 13405

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Function(s)

dystroglycan binding actin binding structural constituent of cytoskeleton protein binding zinc ion binding structural constituent of muscle myosin binding vinculin binding nitric-oxide synthase binding metal ion binding

Process(es)

positive regulation of cell-matrix adhesion regulation of heart rate regulation of transcription, DNA-templated muscle organ development skeletal muscle tissue development establishment of blood-nerve barrier regulation of gene expression regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion positive regulation of neuron projection development regulation of skeletal muscle contraction by regulation of release of sequestered calcium ion regulation of skeletal muscle contraction myotube cell development muscle attachment olfactory nerve structural organization negative regulation of peptidyl-serine phosphorylation cellular protein localization response to muscle stretch regulation of membrane potential peptide biosynthetic process cellular protein complex assembly neurotransmitter receptor metabolic process positive regulation of neuron differentiation muscle cell cellular homeostasis muscle fiber development nucleus localization cardiac muscle contraction regulation of ryanodine-sensitive calcium-release channel activity establishment of glial blood-brain barrier negative regulation of ERK1 and ERK2 cascade cardiac muscle cell action potential regulation of cellular response to growth factor stimulus regulation of voltage-gated calcium channel activity negative regulation of peptidyl-cysteine S-nitrosylation positive regulation of sodium ion transmembrane transporter activity cytoskeleton organization muscle filament sliding

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Dystrophin Polyclonal Antibody

Dystrophin Antibody (PA5-32388) in IHC (P)

Product Details

PA5-32388

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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