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Invitrogen
E-Cadherin Monoclonal Antibody (4A2C7)
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FIGURE: 1 / 20
E-Cadherin Antibody (18-0223) in ICC/IF
Nature communications 2020 -
Fig. 7 CAF-S1 and CAF-S4 promote cancer cell invasion by TGFbeta, CXCL12 and NOTCH. a Up, Representative images of E-cadherin (red), F-actin (green) and DAPI (blue) in BC cells alone (-) or with CAF-S1 transfected with non-targeting (siCTL) or CXCL12-targeting (siCXCL12) siRNA. CAF-S4 images in Supplementary Fig. 5c . Scale bars, 20 mum; inset 10 mum. Down, BC cell density and E-cadherin staining alone (gra... View More
Product Details
18-0223
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
Class
Monoclonal
Type
Antibody
Clone
4A2C7
Immunogen
Recombinant protein corresponding to the cytoplasmic domain of human E-cadherin
Conjugate
Form
Liquid
Purification
purified
Storage buffer
PBS with 1% BSA
Contains
0.1% sodium azide
Storage conditions
2-8°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2925243
Product Specific Information
This monoclonal antibody reacts with human epithelial cadherin (E-cadherin, uvomorulin, Cell-CAM 120/80), a 120 kDa transmembrane glycoprotein. Reduction and/or loss of E-cadherin expression in carcinomas correlates positively with tumor cell invasion and metastasis. Altered expression of E-cadherin is common in the progression of cancers including breast carcinoma, lung carcinoma, gastric tumors, hepatocellular carcinoma, and prostatic tumors. It has recently been reported that E-cadherin is a useful marker for differentiating lobular lesions (negative) from ductal invasive lesions (positive) in breast cancer. Positive tissue: Non-neural epithelia. Reacts strongly with human E-cadherin and weakly with human P-cadherin.
Expected Staining Pattern: Mainly cellular membrane, some cytoplasmic
Target Information
E-Cadherin (epithelial cadherin) is a classical cadherin from the cadherin (alcium dependent adhesion protein) superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. E-cadherin plays a central role in the growth and development of cells by controlling tissue architecture, and maintenance of tissue integrity. In humans, E-cadherin is encoded by the CDH1 gene present on chromosome 16. Studies have demonstrated that reduction and/or loss of E-cadherin expression in carcinomas correlates positively with the potential of these tumors for invasion and metastasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: cadherin 1, E-cadherin (epithelial); cadherin 1, type 1; cadherin 1, type 1, E-cadherin (epithelial); cadherin e; Cadherin-1; cadherin-E; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324; cell-CAM 120/80; E-Cadherin; E-cadherin 1; Ecadherin; Epithelial cadherin; Uvomorulin
Gene Aliases: Arc-1; CD324; CDH1; CDHE; ECAD; LCAM; UVO
UniProt ID: (Human) P12830
Entrez Gene ID: (Human) 999
homophilic cell adhesion via plasma membrane adhesion molecules
synapse assembly
response to toxic substance
single organismal cell-cell adhesion
pituitary gland development
negative regulation of cell-cell adhesion
extracellular matrix disassembly
extracellular matrix organization
neuron projection development
adherens junction organization
response to drug
positive regulation of transcription factor import into nucleus
positive regulation of transcription, DNA-templated
cellular response to lithium ion
cellular response to indole-3-methanol
protein localization to plasma membrane
establishment of protein localization to plasma membrane
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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