Proteintech

E-cadherin Polyclonal Antibody

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View all (149) E-cadherin antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

20874-1-AP

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:4,000
View 1,301 publications 1,301 publications

Immunohistochemistry (IHC)

-
View 246 publications 246 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:500-1:2,000
-

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:20-1:200
-

Immunocytochemistry (ICC/IF)

1:20-1:200
View 281 publications 281 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells
View 3 publications 3 publications

Immunoprecipitation (IP)

0.5-4.0 µg
View 5 publications 5 publications

Miscellaneous PubMed (MISC)

-
View 14 publications 14 publications

Not applicable (N/A)

-
View 20 publications 20 publications
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Bovine, Human, Mouse, Pig, Rat, Zebrafish

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E-cadherin Fusion Protein Ag14973 (373-622 aa encoded by BC141838)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.34 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This polyclonal antibody recognizes 120 kDa intact E-cadherin and its 80 kDa trypsin-cleaved fragment.

Immunogen sequence: PIFNPTTYK GQVPENEANV VITTLKVTDA DAPNTPAWEA VYTILNDDGG QFVVTTNPVN NDGILKTAKG LDFEAKQQYI LHVAVTNVVP FEVSLTTSTA TVTVDVLDVN EAPIFVPPEK RVEVSEDFGV GQEITSYTAQ EPDTFMEQKI TYRIWRDTAN WLEINPDTGA ISTRAELDRE DFEHVKNSTY TALIIATDNG SPVATGTGTL LLILSDVNDN APIPEPRTIF FCERNPKPQV INIIDADLPP I (373-622 aa encoded by BC141838)

Target Information

E-Cadherin (epithelial cadherin) is a classical cadherin from the cadherin (alcium dependent adhesion protein) superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. E-cadherin plays a central role in the growth and development of cells by controlling tissue architecture, and maintenance of tissue integrity. In humans, E-cadherin is encoded by the CDH1 gene present on chromosome 16. Studies have demonstrated that reduction and/or loss of E-cadherin expression in carcinomas correlates positively with the potential of these tumors for invasion and metastasis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: ARC-1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1; cadherin 1, type 1, E-cadherin (epithelial); cadherin e; Cadherin-1; cadherin-E; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324; cell-CAM 120/80; E-Cadherin; E-cadherin 1; Ecadherin; Epithelial cadherin; Uvomorulin

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Gene Aliases: AA960649; Arc-1; CD324; CDH1; CDHE; E-cad; ECAD; L-CAM; LCAM; Um; UVO

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UniProt ID: (Human) P12830, (Mouse) P09803, (Rat) Q9R0T4

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Entrez Gene ID: (Human) 999, (Mouse) 12550, (Rat) 83502

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Function(s)
glycoprotein binding calcium ion binding protein binding beta-catenin binding ankyrin binding GTPase activating protein binding gamma-catenin binding cell adhesion molecule binding cadherin binding involved in cell-cell adhesion protein phosphatase binding protein domain specific binding metal ion binding
Process(es)
in utero embryonic development trophectodermal cell differentiation epithelial cell morphogenesis cell adhesion homophilic cell adhesion via plasma membrane adhesion molecules synapse assembly sensory perception of sound single organismal cell-cell adhesion calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules protein metabolic process negative regulation of cell-cell adhesion regulation of protein localization regulation of water loss via skin adherens junction organization positive regulation of transcription factor import into nucleus regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of transcription, DNA-templated regulation of epithelial cell proliferation negative regulation of epithelial cell proliferation protein homooligomerization intestinal epithelial cell development salivary gland cavitation regulation of branching involved in salivary gland morphogenesis bicellular tight junction assembly cellular response to amino acid stimulus cellular response to lithium ion cellular response to indole-3-methanol protein localization to plasma membrane establishment of protein localization to plasma membrane negative regulation of canonical Wnt signaling pathway cochlea development regulation of protein localization to cell surface regulation of neuron migration response to toxic substance response to organic substance pituitary gland development neuron projection development response to drug extracellular matrix disassembly extracellular matrix organization
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