Hamburger Menu Button
Thermo Fisher Scientific Logo
Sign in
Don't have an account ? Create Account
  • Products
    • Antibodies
    • Cell Culture Media
    • Chemicals
    • Chromatography Columns and Cartridges
    • Lab Equipment
    • Lab Plasticware and Supplies
    • Microplates
    • Oligos, Primers, Probes and Genes
    • TaqMan Real-Time PCR Assays
    • Greener Products
    • See all product categories
  • Applications
    • Bioprocessing
    • Cell Culture and Transfection
    • Cell and Gene Therapy
    • Chromatography
    • Molecular Testing
    • Digital Solutions
    • DNA and RNA Extraction and Analysis
    • Spectroscopy, Elemental and Isotope Analysis
    • See all applications and techniques
  • Services
    • 360° CDMO and CRO Solutions
    • CDMO Services
    • CRO Services
    • Custom Services
    • Enterprise Services
    • Financial and Leasing Services
    • Instrument Services
    • Lab Informatics
    • OEM and Commercial Supply
    • Training Services
    • Unity Lab Services
    • See all services
  • Help and Support
    • How to Order
    • Instrument Support
    • Learning Centers
    • Register for an Account
    • Technical Support Centers
    • See all help and support topics
  • Popular
    • TaqMan Real-Time PCR Assays
      TaqMan Real-Time PCR Assays
    • Antibodies
      Antibodies
    • Oligos, Primers & Probes
      Oligos, Primers & Probes
    • GeneArt Gene Synthesis
      GeneArt Gene Synthesis
    • Cell Culture Plastics
      Cell Culture Plastics
  • Contact Us
  • Quick Order
  • Order Status and Tracking
  • Documents and Certificates
Thermo Fisher Scientific Logo

Search Thermo Fisher Scientific

Search All
Search button Close
          • Order Status
          • Quick Order
          • Sign in
            Sign in
            Don't have an account ? Create Account
            • Account
            • Check Order Status
            • Aspire Member Program
            • Connect: Lab, Data, Apps
            • Custom Products & Projects
            • Services Central

          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • E-cadherin Antibodies

          Invitrogen

          E-cadherin Monoclonal Antibody (4A2C7)

          Advanced Verification
          187 References
          View all (149) E-cadherin antibodies

          Promotion

          View available promotion(s)

          Promo code: {{promo.promoCode}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite E-cadherin Monoclonal Antibody (4A2C7)

          • Antibody Testing Data (2)
          • Advanced Verification (1)
          E-cadherin Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          E-cadherin Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 3

          {{ $ctrl.currentElement.advancedVerification.fullName }}
          {{ $ctrl.currentElement.advancedVerification.text }}

          E-cadherin Antibody (33-4000) in WB

          Western blot was performed using Anti-E-cadherin Monoclonal Antibody (4A2C7) (Product # 33-4000) and a 110kDa band corresponding to E-cadherin was observed across in all tested cell lines and tissues, except MDA-MB-231, SW480 and BJ. Whole cell extracts (30 µg lysate) of MCF7 (Lane 1), MDA-MB-231 (Lane 2), SW480 (Lane 3), Caco-2 (Lane 4), BJ (Lane 5), Mouse Stomach (Lane 6), Rat Stomach (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          View Product

          {{$ctrl.videoDesc}}

          {{$ctrl.videoLongDesc}}

          E-cadherin Antibody in Western Blot (WB)
          E-cadherin Antibody in Western Blot (WB)
          E-cadherin Antibody

          Product Details

          33-4000

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1 µg/mL
          View 28 publications 28 publications

          Immunohistochemistry (IHC)

          2-10 µg/mL
          View 36 publications 36 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          -
          View 52 publications 52 publications

          Immunohistochemistry (Frozen) (IHC (F))

          -
          View 2 publications 2 publications

          Immunocytochemistry (ICC/IF)

          -
          View 16 publications 16 publications

          Immunoprecipitation (IP)

          2-5 µg
          -

          ChIP assay (ChIP)

          -
          View 1 publication 1 publication

          Miscellaneous PubMed (MISC)

          -
          View 52 publications 52 publications
          Product Specifications

          Species Reactivity

          Human

          Published species

          Avian, Dog, Hamster, Human, Mouse, Non-human primate, Pig

          Host/Isotype

          Mouse / IgG1, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          4A2C7

          Immunogen

          Recombinant MBP fusion protein corresponding to the cytoplasmic domain of human E-cadherin

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Purification

          Protein A

          Storage buffer

          PBS, pH 7.4

          Contains

          0.1% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2533118

          Product Specific Information

          This antibody is specific for E-cadherin but does show some minor cross-reactivity with P-cadherin.

          Formalin-fixed, paraffin embedded tissue sections required a Heat Induced Epitope Retrieval (HIER) step prior to staining with this antibody in an IHC application.

          Target Information

          E-Cadherin (epithelial cadherin) is a classical cadherin from the cadherin (alcium dependent adhesion protein) superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. E-cadherin plays a central role in the growth and development of cells by controlling tissue architecture, and maintenance of tissue integrity. In humans, E-cadherin is encoded by the CDH1 gene present on chromosome 16. Studies have demonstrated that reduction and/or loss of E-cadherin expression in carcinomas correlates positively with the potential of these tumors for invasion and metastasis.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: cadherin 1, E-cadherin (epithelial); cadherin 1, type 1; cadherin 1, type 1, E-cadherin (epithelial); cadherin e; Cadherin-1; cadherin-E; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324; cell-CAM 120/80; E-Cadherin; E-cadherin 1; Ecadherin; Epithelial cadherin; Uvomorulin

          View more View less

          Gene Aliases: Arc-1; CD324; CDH1; CDHE; ECAD; LCAM; UVO

          View more View less

          UniProt ID: (Human) P12830

          View more View less

          Entrez Gene ID: (Human) 999

          View more View less

          Function(s)
          glycoprotein binding calcium ion binding protein binding beta-catenin binding ankyrin binding GTPase activating protein binding gamma-catenin binding cell adhesion molecule binding cadherin binding involved in cell-cell adhesion
          Process(es)
          homophilic cell adhesion via plasma membrane adhesion molecules synapse assembly response to toxic substance single organismal cell-cell adhesion pituitary gland development negative regulation of cell-cell adhesion extracellular matrix disassembly extracellular matrix organization neuron projection development adherens junction organization response to drug positive regulation of transcription factor import into nucleus positive regulation of transcription, DNA-templated cellular response to lithium ion cellular response to indole-3-methanol protein localization to plasma membrane establishment of protein localization to plasma membrane
          It has to be done as per old AB suggested Products section.
          guarantee_icon

          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

          Learn more
          help_icon

          We're here to help

          Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

          Contact tech support
          Ordering Plus Icon Minus Icon
          • Order Status
          • Order Help
          • Quick Order
          • Supply Center
          • eProcurement
          Support Plus Icon Minus Icon
          • Help and Support
          • Contact Us
          • Technical Support Centers
          • Documents and Certificates
          • Report a Site Issue
          Resources Plus Icon Minus Icon
          • Learning Centers
          • Promotions
          • Events and Webinars
          • Social Media
          About Thermo Fisher Plus Icon Minus Icon
          • About Us
          • Careers
          • Investors
          • News
          • Social Responsibility
          • Trademarks
          • Consumer Health Data Privacy Policy
          Our Portfolio Plus Icon Minus Icon
          • Thermo Scientific
          • Applied Biosystems
          • Invitrogen
          • Gibco
          • Ion Torrent
          • Fisher Scientific
          • Unity Lab Services
          • Patheon
          • PPD
          • Terms & Conditions
          • Privacy Information Center
          • Price & Freight Policy
          © 2006-2025 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
          United States flag icon
          United States

          Your items have has been added!


          Host server : magellan-search-67c77f6dc7-n6nf2:80/100.66.135.186:80.
          git-commit: 545e792480003146e539d624bd2a21b51d198228
          git-url: https://github.com/thermofisher/magellan-search
          git-branch: release/2.31.0-2025.06.23-1.0