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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • E-cadherin Antibodies

          Invitrogen

          E-cadherin Monoclonal Antibody (SHE78-7)

          Advanced Verification
          22 Published Figures
          59 References
          View all (149) E-cadherin antibodies

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          Datasheet
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          Datasheet
          Protocols
          Questions & Answers

          Cite E-cadherin Monoclonal Antibody (SHE78-7)

          • Antibody Testing Data (1)
          • Published Figures (22)
          • Advanced Verification (3)
          E-cadherin Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          E-cadherin Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 26

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          E-cadherin Antibody (13-5700) in WB

          Western blot was performed using Anti-E-cadherin Monoclonal Antibody (SHE78-7)(Product # 13-5700) and a 110kDa band corresponding to E-cadherin was observed across in all tested cell lines and tissues, except MDA-MB-231, SW480 and BJ. Whole cell extracts (30 µg lysate) of MCF7 (Lane 1), T-47D (Lane 2), MDA-MB-231 (Lane 3), SW480 (Lane 4), Caco-2 (Lane 5), BJ (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001)... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          View Product

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          E-cadherin Antibody in Western Blot (WB)
          E-cadherin Antibody in Immunocytochemistry (ICC/IF)
          E-cadherin Antibody in Immunocytochemistry (ICC/IF)
          E-cadherin Antibody in Immunocytochemistry (ICC/IF)
          E-cadherin Antibody in Immunocytochemistry (ICC/IF)
          E-cadherin Antibody in Immunocytochemistry (ICC/IF)
          E-cadherin Antibody in Immunocytochemistry (ICC/IF)
          E-cadherin Antibody in Immunocytochemistry (ICC/IF)
          E-cadherin Antibody in Immunocytochemistry (ICC/IF)
          E-cadherin Antibody in Western Blot (WB)
          E-cadherin Antibody in Western Blot (WB)
          E-cadherin Antibody in Western Blot (WB)
          E-cadherin Antibody in Western Blot (WB)
          E-cadherin Antibody in Western Blot (WB)
          E-cadherin Antibody in Western Blot (WB)
          E-cadherin Antibody in Western Blot, Immunoprecipitation (WB, IP)
          E-cadherin Antibody in Immunoprecipitation (IP)
          E-cadherin Antibody in Neutralization (Neu)
          E-cadherin Antibody in Neutralization (Neu)
          E-cadherin Antibody in Neutralization (Neu)
          E-cadherin Antibody in Neutralization (Neu)
          E-cadherin Antibody in Neutralization (Neu)
          E-cadherin Antibody in Functional Assay (Functional)
          E-cadherin Antibody
          E-cadherin Antibody
          E-cadherin Antibody

          Product Details

          13-5700

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          2 µg/mL
          View 15 publications 15 publications

          Immunohistochemistry (IHC)

          -
          View 1 publication 1 publication

          Immunohistochemistry (Paraffin) (IHC (P))

          -
          View 1 publication 1 publication

          Immunohistochemistry (Frozen) (IHC (F))

          2 µg/mL
          -

          Immunocytochemistry (ICC/IF)

          -
          View 8 publications 8 publications

          Flow Cytometry (Flow)

          1 µg/mL
          View 2 publications 2 publications

          ELISA (ELISA)

          2 µg/mL
          -

          Immunoprecipitation (IP)

          -
          View 2 publications 2 publications

          Neutralization (Neu)

          -
          View 9 publications 9 publications

          Functional Assay (Functional)

          -
          View 1 publication 1 publication

          Inhibition Assays (IA)

          1 µg/mL
          -

          Miscellaneous PubMed (MISC)

          -
          View 20 publications 20 publications
          Product Specifications

          Species Reactivity

          Human

          Published species

          Bovine, Human, Mouse

          Host/Isotype

          Mouse / IgG2a

          Class

          Monoclonal

          Type

          Antibody

          Clone

          SHE78-7

          Immunogen

          Human placenta.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Lyophilized

          Concentration

          2.0 mg/mL

          Purification

          Ion-exchange chromatography

          Storage buffer

          PBS, pH 7.4, with 1% BSA

          Contains

          no preservative

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2533022

          Product Specific Information

          Source: Monoclonal antibody was obtained by fusing the P3U1 myeloma cell line with spleen cells of BALB/c mouse after immunization with soluble E-cadherin from human placenta.

          Specificity: This antibody specifically reacts with human E-cadherin. This antibody inhibits E-cadherin dependent cell-cell contact.

          Cross reactivity: This antibody does not react with rat E-cadherin.

          Storage: 4°C This product does not contain preservative. The stock solution(2.0 mg/mL) should be stored in aliquots at -20°C for 1 year, or should be stored at 4°C for 6 months after adding 0.1% sodium azide. Avoid repeated freeze-thaw cycles.

          Reconstitution: Dissolve the lyophilized antibody in 50 µL of distilled water (final concentration: 2.0 mg/mL). This solution can be used as a stock solution. If dilution is necessary for your application, dilute the stock solution with the following Dilution solution just prior to use. When the entire amount of antibody is to be used over a short time period, it may be dissolved directly in 500 µL or more of the Dilution solution. Be sure to store the antibody at a minimum concentration of 2.0 mg/mL. A lower antibody concentration may result in decreased stability. Reconstituted antibody solution should contain 0.1% sodium azide as a preservative when stored at 4°C.

          Dilution solution:
          20 mM TBS (pH 7.5) *1
          10 mM CaCl2
          1.0% BSA
          (0.1% NaN3) *2
          *1 TBS should be used for dilution, as PBS causes precipitation.
          *2 When stored at 4C, 0.1% sodium azide should be added as
          a preservative.

          Ca2+ should be contained in the buffers to stabilize cadherin antigen.

          Target Information

          E-Cadherin (epithelial cadherin) is a classical cadherin from the cadherin (alcium dependent adhesion protein) superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. E-cadherin plays a central role in the growth and development of cells by controlling tissue architecture, and maintenance of tissue integrity. In humans, E-cadherin is encoded by the CDH1 gene present on chromosome 16. Studies have demonstrated that reduction and/or loss of E-cadherin expression in carcinomas correlates positively with the potential of these tumors for invasion and metastasis.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: cadherin 1, E-cadherin (epithelial); cadherin 1, type 1; cadherin 1, type 1, E-cadherin (epithelial); cadherin e; Cadherin-1; cadherin-E; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324; cell-CAM 120/80; E-Cadherin; E-cadherin 1; Ecadherin; Epithelial cadherin; Uvomorulin

          View more View less

          Gene Aliases: Arc-1; CD324; CDH1; CDHE; ECAD; LCAM; UVO

          View more View less

          UniProt ID: (Human) P12830

          View more View less

          Entrez Gene ID: (Human) 999

          View more View less

          Function(s)
          glycoprotein binding calcium ion binding protein binding beta-catenin binding ankyrin binding GTPase activating protein binding gamma-catenin binding cell adhesion molecule binding cadherin binding involved in cell-cell adhesion
          Process(es)
          homophilic cell adhesion via plasma membrane adhesion molecules synapse assembly response to toxic substance single organismal cell-cell adhesion pituitary gland development negative regulation of cell-cell adhesion extracellular matrix disassembly extracellular matrix organization neuron projection development adherens junction organization response to drug positive regulation of transcription factor import into nucleus positive regulation of transcription, DNA-templated cellular response to lithium ion cellular response to indole-3-methanol protein localization to plasma membrane establishment of protein localization to plasma membrane
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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