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Rockland
E.coli HCP Polyclonal Antibody
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FIGURE: 1 / 3
E.coli HCP Antibody (100-401-J07) in WB
Western Blot of Rabbit anti-HCP antibody. Lane 1: Molecular Weight. Lane 2: Total HCP. Load: 10µg per lane. Primary antibody: Rabbit anti-HCP cocktail at 1:1000 for overnight at 4°C. Secondary antibody: Goat anti-rabbit secondary antibody at 1:10,000 for 30 min at RT. Block: MB-070 for 1 hour at RT.
Product Details
100-401-J07
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bacteria
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-Host Cell Proteins Antibody was prepared blending antibodies that have been separately developed by repeated immunizations with a mixture of high molecular weight (HMW) and a mixture of low molecular weight (LMW) proteins from E.Coli (BL21).
Conjugate
Form
Liquid
Concentration
77.5 mg/mL
Storage buffer
0.02M potassium phosphate/whole serum, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
E.coli HCP Antibody is directed against high molecular weight and low molecular weight E.coli Host Cell Proteins. The anti-sera reactivity is directed against E.coli Host Cell Proteins. Cross-reactivity with other species is likely but has not been determined. Reactivity against homologues from other sources is not known.
Target Information
Host Cell Proteins are process-related impurities derived from a host cell expression system that may be present in trace amounts in a final drug substance. HCP Antibodies are used to detect impurities in biologically-based therapeutics. Detecting impurities often unseen by 1-D electrophoresis, a 2D electrophoresis western blot analysis delivers more complete insight into the immunocoverage of protein components of the host cell lysate.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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