Invitrogen

E. coli serotype O/K Polyclonal Antibody

E. coli serotype O/K Antibody in ELISA (ELISA)

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E. coli serotype O/K Antibody (PA1-7213) in ELISA

Biosensors 2022 - Figure 3 ( A ) Testing the binding abilities of different polyclonal Abs with an in-house ELISA test with different amounts of intact E. coli cells as antigen. Black: Bio-Rad 4329-4906, white: Thermo Fisher PA1-7213, grey: Thermo Fisher PA1-73032. ( B ) The binding ability of a monoclonal Ab (Bio-Rad OBT0749) with an in-house ELISA test with different amounts of intact E. coli cells as antigen. For detectio... View More

E. coli serotype O/K Antibody in Functional Assay (Functional)

E. coli serotype O/K Antibody in SDS-PAGE (SDS-PAGE)

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Product Details

PA1-7213

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 2 publications 2 publications

ELISA (ELISA)

Assay-dependent

View 5 publications 5 publications

Functional Assay (Functional)

View 1 publication 1 publication

SDS-PAGE (SDS-PAGE)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bacteria

Published species

Bacteria

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Mixtures of all antigenic serotypes.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

4-5 mg/mL

Purification

IgG fraction

Storage buffer

PBS, pH 7.2

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_558822

Product Specific Information

PA1-7213 detects all "O" and "K" antigenic serotypes of Escherichia coli. This antibody is unabsorbed and will cross react with related enterobacteriaceae.

PA1-7213 is suggested for use in ELISA procedures.

The PA1-7213 immunogen is mixtures of all antigenic serotypes.

Recommended short term (6 months) storage is liquid at 2-8 ºC. For longer term storage, aliquot and freeze.

Target Information

Escherichia coli (E. coli) are Gram negative enteric bacteria that live in the human gut. Its presence in nature is an indication of human feacal pollution. E. coli also causes human disease such as urinary tract infections and neonatal meningitis. Infection with particular enterotoxigenic strains can lead to life threatening intestinal diseases. E. coli are distinguished immunologically by serotyping based on three types of antigen: the somatic or O antigen which corresponds to terminal sugars on the cell surface lipopolysaccharide, the capsular or K antigens and the flagellar or H antigens.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Laser Reduced Graphene Oxide Electrode for Pathogenic Escherichia coli Detection.

ACS applied materials & interfaces

Zhao L,Rosati G,Piper A,de Carvalho Castro E Silva C,Hu L,Yang Q,Della Pelle F,Alvarez-Diduk RR,Merkoçi A

PA1-7213 was used in ELISA to report a simple, fast, and maskless method for large-scale, low-cost reduced graphene oxide electrode fabrication; using direct writing (laser scribing and inkjet printing) coupled with a stamp-transferring method.

Tue Feb 14 00:00:00 UTC 2023

Laser Reduced Graphene Oxide Electrode for Pathogenic Escherichia coli Detection.

ACS applied materials & interfaces

Zhao L,Rosati G,Piper A,de Carvalho Castro E Silva C,Hu L,Yang Q,Della Pelle F,Alvarez-Diduk RR,Merkoçi A

Tue Feb 14 00:00:00 UTC 2023

Development and In-Depth Characterization of Bacteria Repellent and Bacteria Adhesive Antibody-Coated Surfaces Using Optical Waveguide Biosensing.

Biosensors

Farkas E,Tarr R,Gerecsei T,Saftics A,Kovács KD,Stercz B,Domokos J,Peter B,Kurunczi S,Szekacs I,Bonyár A,Bányai A,Fürjes P,Ruszkai-Szaniszló S,Varga M,Szabó B,Ostorházi E,Szabó D,Horvath R

PA1-7213 was used in ELISA to develop a method for screening a wide range of bacteria repellent surfaces and characterize the binding performance and affinity of various Ab-based coatings for online label-free monitoring of bacteria.

Thu Jan 20 00:00:00 UTC 2022

Development and In-Depth Characterization of Bacteria Repellent and Bacteria Adhesive Antibody-Coated Surfaces Using Optical Waveguide Biosensing.

Biosensors

Farkas E,Tarr R,Gerecsei T,Saftics A,Kovács KD,Stercz B,Domokos J,Peter B,Kurunczi S,Szekacs I,Bonyár A,Bányai A,Fürjes P,Ruszkai-Szaniszló S,Varga M,Szabó B,Ostorházi E,Szabó D,Horvath R

Thu Jan 20 00:00:00 UTC 2022

Design of combination therapy for engineered bacterial therapeutics in non-small cell lung cancer.

Scientific reports

Deb D,Wu Y,Coker C,Harimoto T,Huang R,Danino T

PA1-7213 was used in ELISA, Immunohistochemistry to design a screening and evaluation pipeline for characterization of bacterial therapies in lung cancer models.

Tue Dec 13 00:00:00 UTC 2022

Blockage of bacterial FimH prevents mucosal inflammation associated with Crohn's disease.

Microbiome

Chevalier G,Laveissière A,Desachy G,Barnich N,Sivignon A,Maresca M,Nicoletti C,Di Pasquale E,Martinez-Medina M,Simpson KW,Yajnik V,Sokol H,Plassais J,Strozzi F,Cervino A,Morra R,Bonny C

PA1-7213 was used in ELISA to propose that TAK-018, which is safe and well tolerated in humans, is a promising candidate for the treatment of CD and in particular in preventing its recurrence. Video abstract.

Mon Aug 23 00:00:00 UTC 2021

Nanoparticle Reservoirs for Paper-Only Immunosensors.

ACS sensors

Alba-Patiño A,Adrover-Jaume C,Rica R

PA1-7213 was used in ELISA to overcome this limitation with a new method for storing protein-decorated nanoparticles on paper substrates that also allows to release them on demand.

Fri Jan 24 00:00:00 UTC 2020

Ultrasensitive and rapid count of Escherichia coli using magnetic nanoparticle probe under dark-field microscope.

BMC microbiology

Xu H,Tang F,Dai J,Wang C,Zhou X

PA1-7213 was used in Western Blot to present an ultrasensitive and on-site method for counting E. coli using magnetic nanoparticle probe under a dark-field in 30 min.

Mon Sep 03 00:00:00 UTC 2018

Ultrasensitive and rapid count of Escherichia coli using magnetic nanoparticle probe under dark-field microscope.

BMC microbiology

Xu H,Tang F,Dai J,Wang C,Zhou X

PA1-7213 was used in Western Blot to present an ultrasensitive and on-site method for counting E. coli using magnetic nanoparticle probe under a dark-field in 30 min.

Mon Sep 03 00:00:00 UTC 2018

Highly sensitive detection of pathogen Escherichia coli O157:H7 by electrochemical impedance spectroscopy.

Biosensors & bioelectronics

Barreiros dos Santos M,Agusil JP,Prieto-Simón B,Sporer C,Teixeira V,Samitier J

PA1-7213 was used in immunohistochemistry to develop a highly sensitive label-free electrochemical impedence spectroscopy method for the detection of pathogenic Escherichia coli 0157:H7

Mon Jul 15 00:00:00 UTC 2013

Quantum dot-based array for sensitive detection of Escherichia coli.

Analytical and bioanalytical chemistry

Sanvicens N,Pascual N,Fernández-Argüelles MT,Adrián J,Costa-Fernández JM,Sánchez-Baeza F,Sanz-Medel A,Marco MP

PA1-7213 was used in ELISA to evaluate a novel method for E. coli detection

Tue Mar 01 00:00:00 UTC 2011

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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E. coli serotype O/K Polyclonal Antibody

E. coli serotype O/K Antibody (PA1-7213) in ELISA

Product Details

PA1-7213

Western Blot (WB)

Immunohistochemistry (IHC)

ELISA (ELISA)

Functional Assay (Functional)

SDS-PAGE (SDS-PAGE)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Performance Guarantee

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