Invitrogen

E. coli serotype O/K Polyclonal Antibody, FITC

E. coli serotype O/K Antibody in Immunocytochemistry (ICC/IF)

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E. coli serotype O/K Antibody (PA1-73029) in ICC/IF

Journal of leukocyte biology 2015 - Figure 6. Formation of LC3B aggregates in the cytoplasm of human neutrophils stimulated with drug-resistant E. coli . Formation of LC3B aggregates was examined under a confocal laser-scanning microscope. Neutrophils were incubated with (+ E. coli ) or without (none) bacteria (A, x200 magnification; B, x600 magnification) and IVIG in the presence of 1% IgG-free serum for 90 minutes. The cells were then exami... View More

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Product Details

PA1-73029

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:10-1:50

View 2 publications 2 publications

ELISA (ELISA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bacteria

Published species

Bacteria, Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

O and K antigenic serotypes (All types) of Escherichia coli.

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

4-5 mg/mL

Purification

IgG fraction

Storage buffer

PBS, pH 7.2, with 10mg/mL BSA

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_1017141

Product Specific Information

The PA1-73029 antibody reacts with E. coli.

PA1-73029 has been successfully used in Immunofluorescence applications.

The PA1-73029 antibody was raised against O and K antigenic serotypes (All types) of Escherichia coli.

Product is light sensitive, please store in the dark.

Target Information

Escherichia coli (E. coli) are Gram negative enteric bacteria that live in the human gut. Its presence in nature is an indication of human feacal pollution. E. coli also causes human disease such as urinary tract infections and neonatal meningitis. Infection with particular enterotoxigenic strains can lead to life threatening intestinal diseases. E. coli are distinguished immunologically by serotyping based on three types of antigen: the somatic or O antigen which corresponds to terminal sugars on the cell surface lipopolysaccharide, the capsular or K antigens and the flagellar or H antigens.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Development and In-Depth Characterization of Bacteria Repellent and Bacteria Adhesive Antibody-Coated Surfaces Using Optical Waveguide Biosensing.

Biosensors

Farkas E,Tarr R,Gerecsei T,Saftics A,Kovács KD,Stercz B,Domokos J,Peter B,Kurunczi S,Szekacs I,Bonyár A,Bányai A,Fürjes P,Ruszkai-Szaniszló S,Varga M,Szabó B,Ostorházi E,Szabó D,Horvath R

PA1-73029 was used in ELISA to develop a method for screening a wide range of bacteria repellent surfaces and characterize the binding performance and affinity of various Ab-based coatings for online label-free monitoring of bacteria.

Thu Jan 20 00:00:00 UTC 2022

Enhancement of neutrophil autophagy by an IVIG preparation against multidrug-resistant bacteria as well as drug-sensitive strains.

Journal of leukocyte biology

Itoh H,Matsuo H,Kitamura N,Yamamoto S,Higuchi T,Takematsu H,Kamikubo Y,Kondo T,Yamashita K,Sasada M,Takaori-Kondo A,Adachi S

PA1-73029 was used in immunocytochemistry to investigate how autophagy and IVIG promote the neutrophil-mediated killing of bacteria

Wed Jul 01 00:00:00 UTC 2015

Enhancement of neutrophil autophagy by an IVIG preparation against multidrug-resistant bacteria as well as drug-sensitive strains.

Journal of leukocyte biology

Itoh H,Matsuo H,Kitamura N,Yamamoto S,Higuchi T,Takematsu H,Kamikubo Y,Kondo T,Yamashita K,Sasada M,Takaori-Kondo A,Adachi S

PA1-73029 was used in immunocytochemistry to investigate how autophagy and IVIG promote the neutrophil-mediated killing of bacteria

Wed Jul 01 00:00:00 UTC 2015

Intravenous immunoglobulin enhances the killing activity and autophagy of neutrophils isolated from immunocompromised patients against multidrug-resistant bacteria.

Biochemical and biophysical research communications

Matsuo H,Itoh H,Kitamura N,Kamikubo Y,Higuchi T,Shiga S,Ichiyama S,Kondo T,Takaori-Kondo A,Adachi S

PA1-73029 was used in immunocytochemistry to identify how intravenous immunoglobulin increases autophagy of neutrophils and killing activity when taken from immunocompromised patients against multi-drug resistant bacteria

Fri Aug 14 00:00:00 UTC 2015

Highly sensitive detection of pathogen Escherichia coli O157:H7 by electrochemical impedance spectroscopy.

Biosensors & bioelectronics

Barreiros dos Santos M,Agusil JP,Prieto-Simón B,Sporer C,Teixeira V,Samitier J

PA1-73029 was used in immunohistochemistry to develop a highly sensitive label-free electrochemical impedence spectroscopy method for the detection of pathogenic Escherichia coli 0157:H7

Mon Jul 15 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: Escherichia coli

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