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Invitrogen
E-selectin Recombinant Rabbit Monoclonal Antibody (103), PE
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E-selectin Antibody (MA5-46667) in Flow
Flow cytometry analysis of E-selectin (CD62E) in HUVEC cells using E-selectin, PE Recombinant Monoclonal (Product # MA5-46667). HUVEC cells (Human umbilical vein endothelial cells) were treated with TNF-alpha (50 ng/mL, 18 hours) prior to staining.
Product Details
MA5-46667
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
103
Immunogen
Recombinant protein ecoding Human E-selectin (Met1-Pro 556).
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
100 µg/mL
Purification
Protein A
Storage buffer
PBS with 0.5% BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2937739
Product Specific Information
Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human E-selectin (CD62E) (Met1-Pro556) and conjugated with PE under optimum conditions, the unreacted PE was removed.
Storage: This antibody can be stored at 2C-8C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
Target Information
Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. In addition, leukocyte adhesion occurs in the capillaries and small venules at any site in the body after onset of inflammation. This response is immediate and involves a cascade of adhesion receptors. At the endothelial surface members of the selectin and immunoglobulin superfamilies participate in this cascade. Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. E-selectin (CD62E) is an endothelial cell specific selectin that is expressed only after activation with proinflammatory cytokines. In vitro experiments have shown that IL-1, TNFalpha and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NFkB dependent signaling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD62 antigen-like family member E; CD62E; e selectin; E-selectin; ELAM-1; endothelial adhesion molecule 1; Endothelial leukocyte adhesion molecule 1; LECAM2; leukocyte endothelial cell adhesion molecule 2; Leukocyte-endothelial cell adhesion molecule 2; RP1-117P20.2
Gene Aliases: CD62E; ELAM; ELAM1; ESEL; LECAM2; SELE
UniProt ID: (Human) P16581
Entrez Gene ID: (Human) 6401
positive regulation of receptor internalization
leukocyte migration involved in inflammatory response
inflammatory response
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
leukocyte cell-cell adhesion
activation of phospholipase C activity
calcium-mediated signaling
actin filament-based process
response to lipopolysaccharide
response to tumor necrosis factor
regulation of inflammatory response
leukocyte migration
leukocyte tethering or rolling
response to interleukin-1
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