Proteintech

E-selectin /CD62E Polyclonal Antibody

E-selectin /CD62E Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 8

E-selectin /CD62E Antibody (20894-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded Human tonsillitis tissue slide using (Product # 20894-1-AP) E-selectin/CD62E antibody at dilution of 1:4,000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

E-selectin /CD62E Antibody in Western Blot (WB)

E-selectin /CD62E Antibody in Flow Cytometry (Flow)

Product Image Gallery

Product Details

20894-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 27 publications 27 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:800

Immunocytochemistry (ICC/IF)

View 5 publications 5 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E-selectin /CD62E Fusion Protein Ag14944 (22-371 aa encoded by BC142711)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.15 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: WSYNTSTEA MTYDEASAYC QQRYTHLVAI QNKEEIEYLN SILSYSPSYY WIGIRKVNNV WVWVGTQKPL TEEAKNWAPG EPNNRQKDED CVEIYIKREK DVGMWNDERC SKKKLALCYT AACTNTSCSG HGECVETINN YTCKCDPGFS GLKCEQIVNC TALESPEHGS LVCSHPLGNF SYNSSCSISC DRGYLPSSME TMQCMSSGEW SAPIPACNVV ECDAVTNPAN GFVECFQNPG SFPWNTTCTF DCEEGFELMG AQSLQCTSSG NWDNEKPTCK AVTCRAVRQP QNGSVRCSHS PAGEFTFKSS CNFTCEEGFM LQGPAQVECT TQGQWTQQIP VCEAFQCTAL S (22-371 aa encoded by BC142711)

Target Information

Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. In addition, leukocyte adhesion occurs in the capillaries and small venules at any site in the body after onset of inflammation. This response is immediate and involves a cascade of adhesion receptors. At the endothelial surface members of the selectin and immunoglobulin superfamilies participate in this cascade. Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. E-selectin (CD62E) is an endothelial cell specific selectin that is expressed only after activation with proinflammatory cytokines. In vitro experiments have shown that IL-1, TNFalpha and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NFkB dependent signaling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CD62 antigen-like family member E; CD62E; e selectin; E-selectin; ELAM-1; endothelial adhesion molecule 1; Endothelial leukocyte adhesion molecule 1; LECAM2; leukocyte endothelial cell adhesion molecule 2; Leukocyte-endothelial cell adhesion molecule 2; RP1-117P20.2

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Gene Aliases: CD62E; ELAM; ELAM1; ESEL; LECAM2; SELE

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UniProt ID: (Human) P16581

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Entrez Gene ID: (Human) 6401

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Function(s)

transmembrane signaling receptor activity protein binding sialic acid binding phospholipase binding oligosaccharide binding

Process(es)

positive regulation of receptor internalization leukocyte migration involved in inflammatory response inflammatory response heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules leukocyte cell-cell adhesion activation of phospholipase C activity calcium-mediated signaling actin filament-based process response to lipopolysaccharide response to tumor necrosis factor regulation of inflammatory response leukocyte migration leukocyte tethering or rolling response to interleukin-1

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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E-selectin /CD62E Polyclonal Antibody

E-selectin /CD62E Antibody (20894-1-AP) in IHC (P)

Product Details

20894-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Miscellaneous PubMed (MISC)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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