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Invitrogen
E2F2 Polyclonal Antibody
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FIGURE: 1 / 1
E2F2 Antibody (PA5-79179) in WB
Western blot analysis of E2F2 in Lane 1: rat lung tissue lysate, Lane 2: rat heart tissue lysate, Lane 3: rat brain tissue lysate, Lane 4: rat kidney tissue lysate, Lane 5: HeLa whole cell lysate, Lane 6: COLO320 whole cell lysate, Lane 7: A549 whole cell lysate, Lane 8: MCF-7 whole cell lysate, Lane 9: SMMC whole cell lysate using 40-50 µg per well. Sample was incubated with E2F2 (Product # PA5-79179) at a dilution of 0.5 µg/mL.
Product Details
PA5-79179
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human E2F2 (422-427aa ISDLFDSYDLGDLLIN).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide, 0.05mg thimerosal
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2746295
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: Rat Lung Tissue, Rat Heart Tissue, Rat Brain Tissue, Rat Kidney Tissue, HELA whole cell, COLO320 whole cell, A549 whole cell, MCF-7 whole cell, SMMC whole cell.
Target Information
E2F2 is a member of the E2Ffamily of transcription factors. The E2F family plays a crucialrole in the control of cell cycle and action of tumor suppressorproteins and is also a target of the transforming proteins of smallDNA tumor viruses. The E2F proteins contain several evolutionallyconserved domains found in most members of the family. Thesedomains include a DNA binding domain, a dimerization domain whichdetermines interaction with the differentiation regulatedtranscription factor proteins (DP), a transactivation domainenriched in acidic amino acids, and a tumor suppressor proteinassociation domain which is embedded within the transactivationdomain. This protein and another 2 members, E2F1 and E2F3, have anadditional cyclin binding domain. This protein binds specificallyto retinoblastoma protein pRB in a cell-cycle dependent manner, andit exhibits overall 46% amino acid identity to E2F1.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: E2F transcription factor 2; E2F-2; Transcription factor E2F2
Gene Aliases: E2F-2; E2F2
UniProt ID: (Human) Q14209, (Mouse) P56931
Entrez Gene ID: (Human) 1870, (Mouse) 242705, (Rat) 684111
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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