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Invitrogen
E2F8 Polyclonal Antibody
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FIGURE: 1 / 3
E2F8 Antibody (PA5-142438) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of E2F8 in U2OS cells using E2F8 Polyclonal Antibody (Product # PA5-142438). Cells were fixed with paraformaldehyde, permeabilized with 0.15% Triton, and incubated with the primary antibody for 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear and cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2... View More
Product Details
PA5-142438
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Peptide with sequence C-KSIESDRRKINSAPS (aa 402-416).
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2933086
Product Specific Information
This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:32,000.
Target Information
E2F transcription factors, such as E2F8, are essential for orchestrating expression of genes required for cell cycle progression and proliferation (Christensen et al., 2005).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: E2F 8; E2F family member 8; E2F-8; FLJ23311; Transcription factor E2F8
Gene Aliases: E2F-8; E2F8
UniProt ID: (Human) A0AVK6
Entrez Gene ID: (Human) 79733
RNA polymerase II core promoter proximal region sequence-specific DNA binding
core promoter binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
transcription corepressor activity
protein binding
protein homodimerization activity
negative regulation of transcription from RNA polymerase II promoter
placenta development
sprouting angiogenesis
transcription, DNA-templated
cell proliferation
negative regulation of cytokinesis
positive regulation of DNA endoreduplication
cell cycle comprising mitosis without cytokinesis
positive regulation of transcription from RNA polymerase II promoter
trophoblast giant cell differentiation
chorionic trophoblast cell differentiation
hepatocyte differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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