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Invitrogen
E3L Polyclonal Antibody
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E3L Antibody (PA5-144504) in WB
Western blot analysis of E3L in recombinant protein using a dilution of 1:2,000. Samples were incubated with E3L polyclonal antibody (Product # PA5-144504). Secondary: Goat polyclonal to rabbit IgG at 1:50,000 dilution. Predicted band size: 28.5 kDa. Observed band size: 29 kDa.
Product Details
PA5-144504
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Virus
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Vaccinia virus Protein E3 (E3L) (1-190AA)
Conjugate
Form
Liquid
Concentration
2.5 mg/mL
Purification
Protein A/G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.03% Proclin 300
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Wet ice
RRID
AB_3090400
Target Information
RNA-binding protein that plays a role in the inhibition of multiple cellular antiviral responses activated by double-stranded RNA (dsRNA), such as inhibition of PKR activation, necroptosis, and IFN-mediated antiviral activities. Recognizes and binds Z-RNA structures via its Z-binding domain and dsRNA via its DRBM domain: RNA-binding activity is required to escape host ZBP1-dependent necroptosis. Mechanistically, the Z-binding domain binds Z-RNAs that are produced during vaccinia virus infection, thereby competing with Z-RNA detection by host ZBP1, suppressing ZBP1-dependent necroptosis. Acts as a key inhibitor of the interferon response by blocking the phosphorylation and subsequent activation of IRF3 and IRF7 kinases that are required for interferon-alpha gene expression. Inhibits NF-kappa-B activation and the ubiquitin-like protein ISG15, which is an early antiviral protein. The binding with host ISG15 subsequently blocks host ISGylation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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