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Invitrogen
E4BP4 (NFIL3) Monoclonal Antibody (S2M-E19), PE, eBioscience™
FIGURE: 1 / 3
E4BP4 (NFIL3) Antibody (12-5927-82) in Flow
Mouse splenocytes unstimulated (left) or stimulated overnight with Mouse IL-15/IL-15R Complex Recombinant Protein Carrier-Free (Product # 34-8152-82) (right) were stained with Anti-Mouse NK1-1 PE-Cy7 (Product # 25-5941-82) and 0.125 µg of Anti-Mouse E4BP4 (NFIL3) PE. Intracellular staining for E4BP4 was performed using the Foxp3 Staining Buffer Set (Product # 00-5523-00) and protocol. Cells in the lymphocyte gate were used for analysis.
Product Details
12-5927-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
S2M-E19
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_11149135
Product Specific Information
Description: This S2M-E19 monoclonal antibody recognizes mouse E4BP4 (NFIL3), a basic leucine zipper transcription factor first identified for its binding to the promoters of the adenovirus E4 protein and human IL-3. E4BP4 is involved in the transcriptional control of the mammalian circadian clock, motor neuron growth and survival, regulation of von Willbrand factor expression, and osteoblast function. In addition, it is also required for the development of NK and NKT cells as well as CD8a+ dendritic cells. Although relatively high levels of mRNA are reported in resting NK and NKT cells, studies at eBioscience have observed expression of E4BP4 protein in lymphocytes only after activation.
Applications Reported: This S2M-E19 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This S2M-E19 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse splenoyctes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Expression of interleukin-3 (IL3; MIM 147740) is restricted to activated T cells, natural killer (NK) cells, and mast cell lines. Transcription initiation depends on the activating capacity of specific protein factors, such as NFIL3, that bind to regulatory regions of the gene, usually upstream of the transcription start site (Zhang et al., 1995 [PubMed 7565758]).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: E4 promoter binding-protein 4; E4 promoter-binding protein 4; Embryo implantation-related NFIL3/E4BP4-like transcription factor; NF IL3A; Nuclear factor interleukin-3-regulated protein; OTTHUMP00000021633
Gene Aliases: AV225605; E4BP4; Nfil3
UniProt ID: (Mouse) O08750
Entrez Gene ID: (Mouse) 18030
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
sequence-specific DNA binding
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
immune response
circadian rhythm
positive regulation of gene expression
rhythmic process
cellular response to interleukin-4
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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