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Alomone Labs, LTD
EAAT2 (GLT-1) (extracellular) Polyclonal Antibody
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FIGURE: 1 / 4
EAAT2 (GLT-1) (extracellular) Antibody (AGC-022-200UL) in ICC/IF
Expression of EAAT2 in human U-87 MG cells - Cell surface detection of EAAT2 in live intact human U-87 MG glioblastoma cells. A. Extracellular staining of cells using Anti-EAAT2 (GLT-1) (extracellular) Antibody (#AGC-022), (1:100) followed by goat Anti-rabbit-Alexa-Fluor-594 secondary Antibody (red). B. Live image of the cells. C. Merge of A and B.
Product Details
AGC-022-200UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
(C)KQLGPGKKNDEVS, corresponding to amino acid residues151-163 of rat EAAT2, 2nd extracellular loop
Conjugate
Form
Lyophilized
Concentration
0.8 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 X g 5 min).
Target Information
Excitatory amino acid transporter 2 (EAAT2) is a member of a family of solute transporter proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors. Mutations in and decreased expression of this protein are associated with amyotrophic lateral sclerosis (ALS.) Alternatively spliced transcript variants of this gene have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EAAT2 variant mEAAT2/5UT5; Excitatory amino acid transporter 2; excitotoxic amino acid transporter 2; glial high affinity glutamate transporter; GLT-1; GLT1a; GLT1b; GLT1c; GLUT-R; glutamate transporter 1c; Glutamate/aspartate transporter II; high affinity glucose transporter; OTTHUMP00000165209; OTTHUMP00000165210; Sodium-dependent glutamate/aspartate transporter 2; solute carrier family 1 (glial high affinity glutamate transporter), member 2; Solute carrier family 1 member 2; solute carrier family 1, member 2
Gene Aliases: 1700091C19Rik; 2900019G14Rik; AI159670; EAAT2; Glt; GLT-1; GLT1; HBGT; MGLT1; SLC1A2
UniProt ID: (Human) P43004, (Mouse) P43006, (Rat) P31596
Entrez Gene ID: (Human) 6506, (Mouse) 20511, (Rat) 29482
transport
dicarboxylic acid transport
nervous system development
visual behavior
response to light stimulus
response to wounding
multicellular organism aging
L-glutamate transport
telencephalon development
adult behavior
cellular response to extracellular stimulus
multicellular organism growth
sodium ion transmembrane transport
response to drug
response to amino acid
positive regulation of glucose import
L-glutamate import
D-aspartate import
L-glutamate transmembrane transport
neurotransmitter transport
amino acid transmembrane transport
ion transport
chemical synaptic transmission
glutamate secretion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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