Invitrogen

EB1 Monoclonal Antibody (1A11/4)

FIGURE: 1 / 6

EB1 Antibody (41-2100) in WB

Western blot analysis was performed on membrane enriched extract (30 µg lysate) of HCT 116 (Lane 1), HeLa (Lane 2), U-87 MG (Lane 3), SW480 (Lane 4), HT-29 (Lane 5), COLO 205 (Lane 6) and MKN45 (Lane 7).The blot was probed with EB1 Mouse monoclonal Antibody (Product # 41-2100, 2 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 32 kDa band corresponding to EB1 was observed in cell lines tested. Known quantity of protein ... View More

EB1 Antibody in Immunocytochemistry (ICC/IF)

Product Image Gallery

EB1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with EB1 siRNA and loss of signal was observed in Western Blot using Anti-EB1 monoclonal Antibody (Product # 41-2100). {KD}

Product Details

41-2100

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

Assay-dependent

View 2 publications 2 publications

ELISA (ELISA)

Assay-dependent

Product Specifications

Species Reactivity

Dog, Human, Mouse, Rat

Published species

Human

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

1A11/4

Immunogen

Recombinant protein encompassing the N-terminal domain of the human EB1 protein

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533500

Target Information

EB1 family proteins are evolutionarily conserved proteins that bind microtubule plus-ends and centrosomes and regulate the dynamics and organization of microtubules. The MAPRE genes encode the EB1 family proteins. The human MAPRE genes MAPRE1, MAPRE2, and MAPRE3 code for EB1, RP1, and EBF3 proteins, respectively. EB1 family members appear to be expressed ubiquitously. The EB1 (MAPRE1) gene, at 20q11. 2, is fused to MLL in an adult patient with pro-B acute lymphoblastic leukemia. EB1 is a microtubule-associated protein that interacts with the colorectal adenomatous polyposis coli tumor-suppressor protein and plays important roles in regulating microtubule dynamics, cell polarity, and chromosome stability.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Growth cone advance requires EB1 as revealed by genomic replacement with a light-sensitive variant.

eLife

Dema A,Charafeddine R,Rahgozar S,van Haren J,Wittmann T

41-2100 was used in Western Blotting to demonstrate that acute and local optogenetic EB1 inactivation in developing cortical neurons induces microtubule depolymerization in the growth cone periphery and subsequent neurite retraction.

Mon Jan 30 00:00:00 UTC 2023

Human Cytomegalovirus Exploits TACC3 To Control Microtubule Dynamics and Late Stages of Infection.

Journal of virology

Furey C,Astar H,Walsh D

41-2100 was used in Immunocytochemistry to reveal the first instance of viral targeting of TACC3 to control microtubule dynamics and virus spread.

Wed Aug 25 00:00:00 UTC 2021

TACC3 Regulates Microtubule Plus-End Dynamics and Cargo Transport in Interphase Cells.

Cell reports

Furey C,Jovasevic V,Walsh D

Published figure using EB1 monoclonal antibody (Product # 41-2100) in Immunofluorescence

Tue Jan 07 00:00:00 UTC 2020

TACC3 Regulates Microtubule Plus-End Dynamics and Cargo Transport in Interphase Cells.

Cell reports

Furey C,Jovasevic V,Walsh D

Published figure using EB1 monoclonal antibody (Product # 41-2100) in Immunofluorescence

Tue Jan 07 00:00:00 UTC 2020

Bioinformatics

Protein Aliases: adenomatosis polyposis coli binding protein Eb1; adenomatous polyposis coli-binding protein EB1; APC-binding protein EB1; EB1; End-binding protein 1; MGC117374; MGC129946; Microtubule-associated protein RP/EB family member 1; microtubule-associated protein, RP/EB family, member 1

View more View less

Gene Aliases: 5530600P05Rik; AI462499; AI504412; AW260097; BIM1p; D2Ertd459e; EB1; MAPRE1

View more View less

UniProt ID: (Human) Q15691, (Rat) Q66HR2, (Mouse) Q61166

View more View less

Entrez Gene ID: (Human) 22919, (Dog) 100685707, (Rat) 114764, (Mouse) 13589

View more View less

Function(s)

protein binding protein C-terminus binding identical protein binding poly(A) RNA binding microtubule plus-end binding cadherin binding involved in cell-cell adhesion microtubule binding

Process(es)

G2/M transition of mitotic cell cycle sister chromatid cohesion mitotic nuclear division cell proliferation positive regulation of cell migration negative regulation of microtubule polymerization protein localization to microtubule cell division cell-cell adhesion positive regulation of microtubule plus-end binding negative regulation of microtubule binding regulation of microtubule polymerization cell cycle regulation of microtubule plus-end binding

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

EB1 Monoclonal Antibody (1A11/4)

EB1 Antibody (41-2100) in WB

Product Details

41-2100

Western Blot (WB)

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!