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EB1 Antibody (MA5-47663) in ICC/IF
Immunocytochemistry analysis of EB1 in paraformaldehyde-fixed and NP40-permeabilized A431 cells. The cells were dual labeled with mouse monoclonal anti-alpha-Tubulin (left) and rat EB1 monoclonal antibody (Product # MA5-47663) (middle). The antibodies were detected using either goat anti-mouse: DyLight® 488 or goat anti-Rat: DyLight® 594.
Product Details
MA5-47663
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rat
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
KT51
Immunogen
Clone KT51 was generated from a GST fusion protein containing amino acids from the C-terminal region of mouse EB1. This sequence is highly conserved in human and rat EB1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2942644
Product Specific Information
The antibody detects a 30 kDa* protein corresponding to the molecular mass of EB1 on SDS-PAGE immunoblots of human HeLa, mouse brain, and rat PC12 cells. The antibody also detects EB1 by immunocytochemistry in HeLa and A431 cells.
Target Information
EB1 family proteins are evolutionarily conserved proteins that bind microtubule plus-ends and centrosomes and regulate the dynamics and organization of microtubules. The MAPRE genes encode the EB1 family proteins. The human MAPRE genes MAPRE1, MAPRE2, and MAPRE3 code for EB1, RP1, and EBF3 proteins, respectively. EB1 family members appear to be expressed ubiquitously. The EB1 (MAPRE1) gene, at 20q11. 2, is fused to MLL in an adult patient with pro-B acute lymphoblastic leukemia. EB1 is a microtubule-associated protein that interacts with the colorectal adenomatous polyposis coli tumor-suppressor protein and plays important roles in regulating microtubule dynamics, cell polarity, and chromosome stability.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: adenomatosis polyposis coli binding protein Eb1; adenomatous polyposis coli-binding protein EB1; APC-binding protein EB1; EB1; End-binding protein 1; MGC117374; MGC129946; Microtubule-associated protein RP/EB family member 1; microtubule-associated protein, RP/EB family, member 1
Gene Aliases: 5530600P05Rik; AI462499; AI504412; AW260097; BIM1p; D2Ertd459e; EB1; MAPRE1
UniProt ID: (Human) Q15691, (Rat) Q66HR2, (Mouse) Q61166
Entrez Gene ID: (Human) 22919, (Rat) 114764, (Mouse) 13589
G2/M transition of mitotic cell cycle
sister chromatid cohesion
mitotic nuclear division
cell proliferation
positive regulation of cell migration
negative regulation of microtubule polymerization
protein localization to microtubule
cell division
cell-cell adhesion
positive regulation of microtubule plus-end binding
negative regulation of microtubule binding
regulation of microtubule polymerization
cell cycle
regulation of microtubule plus-end binding
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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