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          • Primary Antibodies ›
          • EBP50 Antibodies

          Invitrogen

          EBP50 Polyclonal Antibody

          Advanced Verification
          27 Published Figures
          42 References
          View all (16) EBP50 antibodies

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          Datasheet
          Protocols
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          Datasheet
          Protocols
          Questions & Answers

          Cite EBP50 Polyclonal Antibody

          • Antibody Testing Data (5)
          • Published Figures (27)
          • Advanced Verification (3)
          EBP50 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          EBP50 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 35

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          EBP50 Antibody (PA1-090) in ICC/IF

          Immunofluorescent analysis of EBP50 was performed using 70% confluent log phase T-47D cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with EBP50 Rabbit Polyclonal Antibody (Product # PA1-090) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A2... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          EBP50 Antibody in Immunocytochemistry (ICC/IF)
          EBP50 Antibody in Immunohistochemistry (IHC)
          EBP50 Antibody in Western Blot (WB)
          EBP50 Antibody in Western Blot (WB)
          EBP50 Antibody in Western Blot (WB)
          EBP50 Antibody in Immunocytochemistry (ICC/IF)
          EBP50 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          EBP50 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          EBP50 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          EBP50 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          EBP50 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          EBP50 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          EBP50 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          EBP50 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          EBP50 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          EBP50 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          EBP50 Antibody in Immunohistochemistry (IHC)
          EBP50 Antibody in Immunohistochemistry (IHC)
          EBP50 Antibody in Immunohistochemistry (IHC)
          EBP50 Antibody in Immunohistochemistry (IHC)
          EBP50 Antibody in Immunohistochemistry (IHC)
          EBP50 Antibody in Immunohistochemistry (IHC)
          EBP50 Antibody in Immunohistochemistry (IHC)
          EBP50 Antibody in Western Blot (WB)
          EBP50 Antibody in Western Blot (WB)
          EBP50 Antibody in Western Blot (WB)
          EBP50 Antibody in Western Blot (WB)
          EBP50 Antibody in Western Blot (WB)
          EBP50 Antibody in Western Blot (WB)
          EBP50 Antibody in Western Blot (WB)
          EBP50 Antibody in Western Blot (WB)
          EBP50 Antibody in Western Blot (WB)
          EBP50 Antibody
          EBP50 Antibody
          EBP50 Antibody

          Product Details

          PA1-090

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.2-1 µg/mL
          View 12 publications 12 publications

          Immunohistochemistry (IHC)

          -
          View 18 publications 18 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          10 µg/mL
          View 7 publications 7 publications

          Immunocytochemistry (ICC/IF)

          2 µg/mL
          View 4 publications 4 publications

          Immunoprecipitation (IP)

          Assay-dependent
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human, Mouse

          Published species

          Dog, Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Bacterially expressed, His-tagged human EBP50.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS with 1mg/mL BSA

          Contains

          0.05% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2191493

          Product Specific Information

          PA1-090 detects ezrin-radixin-moesin (ERM) binding phosphoprotein of 50 kDa (EBP50) from mouse tissues, human T84 and Calu3 cells as well as recombinant human protein.

          PA1-090 has been successfully used in Western blot, Immunohistochemistry, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects an ~50 kDa protein representing EBP50 from T84 cell extract. Immunohistochemical staining of EBP50 in mouse airway results in staining of the epithelium.

          The PA1-090 immunogen is recombinant human His-tagged EBP50 obtained from bacteria. All His-tag specific antibody has been removed. This product was epitope affinity purified using a synthetic peptide to residues C R(286) S A S S D T S E E L N S Q D S P(302) of human EBP50.

          Target Information

          NHERF1 (Na+/H+ exchanger regulatory factor 1), also known as EBP50 (ezrin, radixin, moesin-binding phosphoprotein 50) is an adaptor protein, which associates with beta-catenin and is required for its localization at the cell-cell junctions, interacts with various G protein-coupled receptors and regulates their traffic, as well as sodium-hydrogen exchange and sodium-dependent phosphate transport. NHERF1/EBP50 inhibits cell motility and is required to suppress anchorage-independent growth. It contains C-terminal ERM (ezrin, radixin, moesin)-binding region and two N-terminal PDZ (postsynaptic-density-95/disc-large/ZO1 homology) domains and is able to form head-to-tail intramolecular conformation to regulate its interactions.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: EBP50; ezrin-radixin-moesin binding phosphoprotein-50; Ezrin-radixin-moesin-binding phosphoprotein 50; Na(+)/H(+) exchange regulatory cofactor NHE-RF1; Na+/H+ exchange regulatory co-factor; NHERF-1; Regulatory cofactor of Na(+)/H(+) exchanger; So; Sodium-hydrogen exchanger regulatory factor 1; solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 1; solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatory factor 1; Solute carrier family 9 isoform A3 regulatory factor 1; solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 1

          View more View less

          Gene Aliases: EBP-50; EBP50; NHE-RF; NHERF; NHERF-1; NHERF1; NPHLOP2; SLC9A3R1

          View more View less

          UniProt ID: (Human) O14745, (Mouse) P70441

          View more View less

          Entrez Gene ID: (Human) 9368, (Mouse) 26941

          View more View less

          Function(s)
          receptor binding protein binding beta-catenin binding chloride channel regulator activity phosphatase binding PDZ domain binding beta-2 adrenergic receptor binding protein complex binding protein complex scaffold protein self-association dopamine receptor binding growth factor receptor binding protein domain specific binding
          Process(es)
          renal sodium ion transport protein complex assembly adenylate cyclase-activating dopamine receptor signaling pathway sensory perception of sound negative regulation of cell proliferation regulation of cell shape regulation of cell size negative regulation of platelet-derived growth factor receptor signaling pathway negative regulation of phosphatidylinositol 3-kinase signaling Wnt signaling pathway cAMP-mediated signaling gland morphogenesis microvillus assembly actin cytoskeleton organization negative regulation of cell migration cellular phosphate ion homeostasis regulation of sodium:proton antiporter activity negative regulation of sodium:proton antiporter activity bile acid secretion glutathione transport negative regulation of catenin import into nucleus establishment of nucleus localization regulation of excretion establishment of epithelial cell apical/basal polarity regulation of protein kinase activity negative regulation of mitotic cell cycle establishment of Golgi localization negative regulation of protein kinase B signaling auditory receptor cell stereocilium organization phospholipase C-activating dopamine receptor signaling pathway renal absorption negative regulation of ERK1 and ERK2 cascade establishment of protein localization to plasma membrane renal phosphate ion absorption positive regulation of intrinsic apoptotic signaling pathway negative regulation of sodium ion transport cellular protein localization negative regulation of cell motility
          It has to be done as per old AB suggested Products section.
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