Invitrogen

EBP50 Recombinant Rabbit Monoclonal Antibody (JJ0946)

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Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Product Details

MA5-32493

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-
Product Specifications

Species Reactivity

Human, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

JJ0946

Immunogen

Recombinant protein within Human EBP50 aa 1-150
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2809770

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

NHERF1 (Na+/H+ exchanger regulatory factor 1), also known as EBP50 (ezrin, radixin, moesin-binding phosphoprotein 50) is an adaptor protein, which associates with beta-catenin and is required for its localization at the cell-cell junctions, interacts with various G protein-coupled receptors and regulates their traffic, as well as sodium-hydrogen exchange and sodium-dependent phosphate transport. NHERF1/EBP50 inhibits cell motility and is required to suppress anchorage-independent growth. It contains C-terminal ERM (ezrin, radixin, moesin)-binding region and two N-terminal PDZ (postsynaptic-density-95/disc-large/ZO1 homology) domains and is able to form head-to-tail intramolecular conformation to regulate its interactions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: EBP50; ERM-binding phosphoprotein; ezrin-radixin-moesin binding phosphoprotein-50; Ezrin-radixin-moesin-binding phosphoprotein 50; Na(+)/H(+) exchange regulatory cofactor NHE-RF1; Na+/H+ exchange regulatory co-factor; NHERF-1; Regulatory cofactor of Na(+)/H(+) exchanger; So; Sodium-hydrogen exchanger regulatory factor 1; solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 1; solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatory factor 1; solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1; Solute carrier family 9 isoform A3 regulatory factor 1; solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 1

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Gene Aliases: EBP50; NHERF; NHERF-1; NHERF1; NPHLOP2; SLC9A3R1

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UniProt ID: (Human) O14745, (Rat) Q9JJ19

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Entrez Gene ID: (Human) 9368, (Rat) 59114

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Function(s)
receptor binding protein binding beta-catenin binding chloride channel regulator activity phosphatase binding PDZ domain binding beta-2 adrenergic receptor binding protein complex binding protein complex scaffold protein self-association dopamine receptor binding growth factor receptor binding protein domain specific binding
Process(es)
renal sodium ion transport adenylate cyclase-activating dopamine receptor signaling pathway sensory perception of sound negative regulation of cell proliferation negative regulation of platelet-derived growth factor receptor signaling pathway negative regulation of sodium ion transport negative regulation of phosphatidylinositol 3-kinase signaling Wnt signaling pathway cAMP-mediated signaling microvillus assembly actin cytoskeleton organization negative regulation of cell migration cellular phosphate ion homeostasis negative regulation of sodium:proton antiporter activity bile acid secretion cellular protein localization glutathione transport regulation of excretion regulation of protein kinase activity negative regulation of protein kinase B signaling auditory receptor cell stereocilium organization phospholipase C-activating dopamine receptor signaling pathway renal absorption negative regulation of ERK1 and ERK2 cascade renal phosphate ion absorption negative regulation of cell motility positive regulation of intrinsic apoptotic signaling pathway protein complex assembly regulation of cell shape regulation of cell size gland morphogenesis regulation of sodium:proton antiporter activity negative regulation of catenin import into nucleus establishment of nucleus localization establishment of epithelial cell apical/basal polarity negative regulation of mitotic cell cycle establishment of Golgi localization establishment of protein localization to plasma membrane
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