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EDAR Antibody (MA5-51876) in ICC/IF
Immunocytochemistry analysis of EDAR in paraformaldehyde fixed A549 cells , permeabilized with 0.15% Triton using EDAR Recombinant Mouse Monoclocal Antibody (Product # MA5-51876) at 10 µg/mL for 1h followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing weak membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom: primary antibody, DAPI, merged channels and an isotype control. The isotype control was stained with an unknown specificity antibody (Product # MA5-47823) followed by Alexa Fluor488 ... View More
Product Details
MA5-51876
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Chicken,
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
EDAR12
Immunogen
EDAR12 was prepared by immunixzing female OVE1B mice (with the Edar gene deleted) subcutaneously with mouse EDAR-Fc and positive hybridoma clones were screened for binding mEDAR by ELISA.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.02% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3250568
Product Specific Information
EDAR12 recognises and binds to CRD1 of the extracellular domain of mouse EDAR. EDAR3 cross-reacts with EDAR derived from human, dog, rat and chicken when EDAR is fused to the glycosylphosphatidylinositol anchor of TRAILR3. EDAR is the receptor for the TNF family ligand EDA1, which is a type II transmembrane protein possessing a collagen-like domain and a C-terminal TNF-homology domain. EDAR is important for the correct development of skin appendages including hair, teeth and eccrine sweat glands. LoF mutations in the Eda gene is known to cause XLHED (X-linked hypohidrotic ectodermal dysplasia), and results in abnormal development.
Target Information
This gene encodes a member of the tumor necrosis factor receptor family. The encoded transmembrane protein is a receptor for the soluble ligand ectodysplasin A, and can activate the nuclear factor-kappaB, JNK, and caspase-independent cell death pathways. It is required for the development of hair, teeth, and other ectodermal derivatives. Mutations in this gene result in autosomal dominant and recessive forms of hypohidrotic ectodermal dysplasia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Anhidrotic ectodysplasin receptor 1; Downless; Downless homolog; downless, mouse, homolog of; Ectodermal dysplasia receptor; ectodysplasin 1, anhidrotic receptor; ectodysplasin A1 receptor; Ectodysplasin-A receptor; EDA-A1 receptor; Tumor necrosis factor receptor superfamily member EDAR
Gene Aliases: DL; ECTD10A; ECTD10B; ED1R; ED3; ED5; EDA-A1R; EDA1R; EDA3; EDAR; HRM1; RGD1561714
UniProt ID: (Human) Q9UNE0, (Mouse) Q9R187
Entrez Gene ID: (Human) 10913, (Dog) 481305, (Chicken) 428015, (Rat) 365581, (Mouse) 13608
hair follicle development
apoptotic process
epidermis development
positive regulation of gene expression
cell differentiation
tumor necrosis factor-mediated signaling pathway
positive regulation of NF-kappaB import into nucleus
odontogenesis of dentin-containing tooth
positive regulation of I-kappaB kinase/NF-kappaB signaling
pigmentation
positive regulation of JNK cascade
salivary gland cavitation
signal transduction
biological_process
multicellular organism development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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