Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
EDB-FN Chimeric Recombinant Rabbit Monoclonal Antibody (A2)
FIGURE: 1 / 1
EDB-FN Chimeric Antibody (MA5-48023) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of EDB-FN in HeLa cells using EDB-FN Chimeric Monoclonal Antibody (Product # MA5-48023). Paraformaldehyde-fixed cells were stained with EDB-FN Chimeric Monoclonal Antibody at 10 µg/mL for 1h followed by Alexa Fluor488 secondary antibody (1 µg/mL), showing endoplasmic reticular staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom ... View More
Product Details
MA5-48023
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG, kappa
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
A2
Immunogen
2D-PAGE gel isolated human Extra-Domain containing fibronectin from lysate of human melanoma COLO-38 cells.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.02% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3074466
Product Specific Information
Specificity: This antibody bind to human Extra-Domain containing fibronectin.
Target Information
EDB-FN is a variant of the fibronectin protein that contains an extra domain, known as the extra domain B. This extra domain is not present in the normal fibronectin protein and has been found to be expressed in certain tissues during embryonic development and in tumors. The extra domain has been implicated in cell adhesion, migration, and invasion, and has been shown to play a role in the progression and metastasis of certain cancers. Due to its unique expression pattern and functional properties, EDB-FN has been investigated as a potential target for cancer therapy and imaging.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: CIG; Cold-insoluble globulin; extra domain B; extra domain B of fibronectin; Fibronectin; FN; migration-stimulating factor
Gene Aliases: CIG; ED-B; FINC; FN; FN1; FNZ; GFND; GFND2; LETS; MSF
UniProt ID: (Human) Q585T2
Entrez Gene ID: (Human) 2335
angiogenesis
cell activation
regulation of protein phosphorylation
platelet degranulation
response to ischemia
acute-phase response
cell-substrate junction assembly
cell adhesion
calcium-independent cell-matrix adhesion
positive regulation of cell proliferation
glial cell migration
regulation of cell shape
response to wounding
response to ozone
positive regulation of gene expression
positive regulation of peptidase activity
peptide cross-linking
extracellular matrix disassembly
extracellular matrix organization
integrin activation
substrate adhesion-dependent cell spreading
cellular response to vascular endothelial growth factor stimulus
endodermal cell differentiation
cellular response to platelet-derived growth factor stimulus
wound healing
negative regulation of apoptotic process
positive regulation of axon extension
positive regulation of fibroblast proliferation
leukocyte migration
positive regulation of chemotaxis
response to glucocorticoid
regulation of ERK1 and ERK2 cascade
cellular response to lipopolysaccharide
cellular response to mercury ion
cellular response to glucose stimulus
cellular response to interleukin-1
cellular response to prostaglandin E stimulus
cellular response to transforming growth factor beta stimulus
cellular response to BMP stimulus
positive regulation of substrate-dependent cell migration, cell attachment to substrate
negative regulation of transforming growth factor-beta secretion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support