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          • Primary Antibodies ›
          • Endothelin 1 Antibodies

          Abnova

          EDN1 Monoclonal Antibody (3D6)

          View all (14) Endothelin 1 antibodies

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          Datasheet
          Protocols
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          Datasheet
          Protocols
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          Cite EDN1 Monoclonal Antibody (3D6)

          • Antibody Testing Data (3)
          EDN1 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          EDN1 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 3

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          EDN1 Antibody (H00001906-M01) in WB

          Western Blot analysis of EDN1 expression in transfected 293T cell line by EDN1 monoclonal antibody (M01), clone 3D6.

          Lane 1: EDN1 transfected lysate(24 KDa).
          Lane 2: Non-transfected lysate.
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          EDN1 Antibody in Western Blot (WB)
          EDN1 Antibody in Immunoprecipitation (IP)
          EDN1 Antibody in ELISA (ELISA)

          Product Details

          H00001906-M01

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1-5 µg/mL
          -

          ELISA (ELISA)

          0.3 ng/mL
          -

          Immunoprecipitation (IP)

          1-5 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Mouse / IgG2a, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          3D6

          Immunogen

          EDN1 (AAH09720, 113 a.a. approximately 212 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          See Label

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.4

          Contains

          no preservative

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          Product Specific Information

          Sequence of this protein is as follows: SQKDKKCWNF CQAGKELRAE DIMEKDWNNH KKGKDCSKLG KKCIYQQLVR GRKIRRSSEE HLRQTRSETM RNSVKSSFHD PKLKGNPSRE RYVTHNRAHW

          Target Information

          The protein encoded by this gene is proteolytically processed to release a secreted peptide termed endothelin 1. This peptide is a potent vasoconstrictor and is produced by vascular endothelial cells. Endothelin 1 also can affect the central nervous system. Two transcript variants encoding different isoforms have been found for this gene.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: Big endothelin 1; Big ET; Big ET 1; Big ET1; BigET; Endothelin-1; Et; PPET1; Preproendothelin-1

          View more View less

          Gene Aliases: ARCND3; EDN1; ET1; HDLCQ7; PPET1; QME

          View more View less

          UniProt ID: (Human) P05305

          View more View less

          Entrez Gene ID: (Human) 1906

          View more View less

          Function(s)
          cytokine activity hormone activity protein binding endothelin A receptor binding endothelin B receptor binding
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter prostaglandin biosynthetic process patterning of blood vessels in utero embryonic development histamine secretion regulation of systemic arterial blood pressure by endothelin regulation of pH cell surface receptor signaling pathway G-protein coupled receptor signaling pathway positive regulation of cytosolic calcium ion concentration protein kinase C-activating G-protein coupled receptor signaling pathway cell-cell signaling heart development respiratory gaseous exchange body fluid secretion positive regulation of cell proliferation dorsal/ventral pattern formation response to ozone multicellular organism aging positive regulation of heart rate positive regulation of endothelial cell migration positive regulation of cardiac muscle hypertrophy positive regulation of receptor biosynthetic process neural crest cell development phosphatidylinositol 3-kinase signaling response to activity artery smooth muscle contraction vein smooth muscle contraction glucose transport cell growth regulation of vasoconstriction sensory perception of pain calcium-mediated signaling peptide hormone secretion nitric oxide transport negative regulation of blood coagulation positive regulation of cell migration neutrophil chemotaxis negative regulation of cAMP biosynthetic process phospholipase D-activating G-protein coupled receptor signaling pathway negative regulation of cellular protein metabolic process positive regulation of prostaglandin secretion response to lipopolysaccharide response to testosterone negative regulation of smooth muscle cell apoptotic process response to prostaglandin F response to nicotine cellular response to drug positive regulation of urine volume positive regulation of renal sodium excretion response to muscle stretch epithelial fluid transport vasoconstriction protein kinase C deactivation middle ear morphogenesis positive regulation of odontogenesis superoxide anion generation rhythmic excitation response to amino acid positive regulation of MAP kinase activity positive regulation of JUN kinase activity response to leptin leukocyte activation positive regulation of nitric oxide biosynthetic process positive regulation of cell size positive regulation of mitotic nuclear division positive regulation of transcription from RNA polymerase II promoter positive regulation of smooth muscle contraction positive regulation of hormone secretion negative regulation of hormone secretion inositol phosphate-mediated signaling positive regulation of smooth muscle cell proliferation positive regulation of sequence-specific DNA binding transcription factor activity cartilage development positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway negative regulation of nitric-oxide synthase biosynthetic process membrane depolarization regulation of sensory perception of pain maternal process involved in parturition positive regulation of sarcomere organization positive regulation of prostaglandin-endoperoxide synthase activity positive regulation of cell growth involved in cardiac muscle cell development positive regulation of chemokine-mediated signaling pathway cellular response to calcium ion cellular response to interferon-gamma cellular response to interleukin-1 cellular response to tumor necrosis factor cellular response to peptide hormone stimulus cellular response to glucocorticoid stimulus cellular response to mineralocorticoid stimulus cellular response to fatty acid cellular response to hypoxia response to dexamethasone cellular response to transforming growth factor beta stimulus response to salt
          It has to be done as per old AB suggested Products section.
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