Invitrogen

EEA1 Monoclonal Antibody (F.43.1)

EEA1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 8

EEA1 Antibody (MA5-14794) in ICC/IF

Immunofluorescent analysis of EEA1 in HeLa cells using an EEA1 monoclonal antibody (Product # MA5-14794) (green). Actin filaments are labeled with a fluorescent red phalloidin. DNA is labeled using a fluorescent blue dye.

Product Image Gallery

EEA1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. A-431 cells were transfected with EEA1 siRNA and decrease in signal intensity was observed in western blot application using Anti-EEA1 Rabbit Monoclonal Antibody (Product # MA5-14794). {KD}

Product Details

MA5-14794

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

1:50-1:200

View 8 publications 8 publications

Immunoprecipitation (IP)

1:100

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Dog, Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

F.43.1

Immunogen

Synthetic peptide corresponding to residues surrounding Ser70 of human EEA1 protein

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

44 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10985824

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

Early endosomal antigen 1 (EEA1) is a 162 kDa membrane bound protein component specific to the early endosome and is essential for fusion between early endocytic vesicles. Early endosomes are cytoplasmic compartments which fuse with endocytic vesicles for redistribution of extracellular compounds to alternate destinations. Zinc-finger-like domains, reminiscent of those found in nucleic acid binding proteins, are located in the amino and carboxyl-terminal domains of EEA1. The carboxyl-terminal zinc-finger-like-domain is conserved in several other non-nuclear proteins, some of which are also involved in intracellular protein trafficking. In addition, this domain is an authentic zinc-binding domain and is critical to the intracellular localization of EEA1. Membrane association of EEA1 has been shown dependent on phosphatidyl 3-kinase activity, inhibitors of which cause EEA1 to dissociate from early endosomes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Lysosomal exocytosis releases pathogenic α-synuclein species from neurons in synucleinopathy models.

Nature communications

Xie YX,Naseri NN,Fels J,Kharel P,Na Y,Lane D,Burré J,Sharma M

MA5-14794 was used in Western Blotting to propose lysosomal exocytosis as a central mechanism for the release of aggregated and degradation-resistant proteins from neurons.

Mon Aug 22 00:00:00 UTC 2022

Interaction of surfactant coated PLGA nanoparticles with in vitro human brain-like endothelial cells.

International journal of pharmaceutics

Moya ELJ,Lombardo SM,Vandenhaute E,Schneider M,Mysiorek C,Türeli AE,Kanda T,Shimizu F,Sano Y,Maubon N,Gosselet F,Günday-Türeli N,Dehouck MP

MA5-14794 was used in Immunocytochemistry-immunofluorescence to investigate two surfactant-coated poly(lactide-co-glycolide) (PLGA) nanoparticles, as formulations for delivery to the brain through the BBB, presenting different uptake profiles.

Fri Jun 10 00:00:00 UTC 2022

Identification of Required Host Factors for SARS-CoV-2 Infection in Human Cells.

Cell

Daniloski Z,Jordan TX,Wessels HH,Hoagland DA,Kasela S,Legut M,Maniatis S,Mimitou EP,Lu L,Geller E,Danziger O,Rosenberg BR,Phatnani H,Smibert P,Lappalainen T,tenOever BR,Sanjana NE

Thu Jan 07 00:00:00 UTC 2021

Identification of Required Host Factors for SARS-CoV-2 Infection in Human Cells.

Cell

Daniloski Z,Jordan TX,Wessels HH,Hoagland DA,Kasela S,Legut M,Maniatis S,Mimitou EP,Lu L,Geller E,Danziger O,Rosenberg BR,Phatnani H,Smibert P,Lappalainen T,tenOever BR,Sanjana NE

Thu Jan 07 00:00:00 UTC 2021

The Epidermal Growth Factor Receptor Is a Relevant Host Factor in the Early Stages of The Zika Virus Life Cycle In Vitro.

Journal of virology

Sabino C,Bender D,Herrlein ML,Hildt E

MA5-14794 was used in Immunocytochemistry-immunoflourescence to demonstrate the relevance of EGFR in the early stages of ZIKV infection and identify EGFR as a target for antiviral strategies.

Mon Sep 27 00:00:00 UTC 2021

Stress Response of Mouse Embryonic Fibroblasts Exposed to Polystyrene Nanoplastics.

International journal of molecular sciences

Han SW,Choi J,Ryu KY

MA5-14794 was used in Western Blotting to indicate that accumulated PS nanoplastics are removed or exported out of the cells.

Sat Feb 20 00:00:00 UTC 2021

Stress Response of Mouse Embryonic Fibroblasts Exposed to Polystyrene Nanoplastics.

International journal of molecular sciences

Han SW,Choi J,Ryu KY

MA5-14794 was used in Western Blotting to indicate that accumulated PS nanoplastics are removed or exported out of the cells.

Sat Feb 20 00:00:00 UTC 2021

Macrophages use a bet-hedging strategy for antimicrobial activity in phagolysosomal acidification.

The Journal of clinical investigation

Dragotakes Q,Stouffer KM,Fu MS,Sella Y,Youn C,Yoon OI,De Leon-Rodriguez CM,Freij JB,Bergman A,Casadevall A

MA5-14794 was used in Immunocytochemistry to analyze the dynamical distribution of phagolysosomal pH in murine and human macrophages that had ingested live or dead Cryptococcus neoformans cells, or inert beads.

Wed Jul 01 00:00:00 UTC 2020

Differential signaling pathways are initiated in macrophages during infection depending on the intracellular fate of Chlamydia spp.

Immunology and cell biology

Nagarajan UM,Tripathy M,Kollipara A,Allen J,Goodwin A,Whittimore J,Wyrick PB,Rank RG

Published figure using EEA1 monoclonal antibody (Product # MA5-14794) in Immunocytochemistry

Thu Mar 01 00:00:00 UTC 2018

Patient-specific mutations impair BESTROPHIN1's essential role in mediating Ca²⁺-dependent Cl^- currents in human RPE.

eLife

Li Y,Zhang Y,Xu Y,Kittredge A,Ward N,Chen S,Tsang SH,Yang T

Published figure using EEA1 monoclonal antibody (Product # MA5-14794) in Immunofluorescence

Tue Oct 24 00:00:00 UTC 2017

Autophagy activation by novel inducers prevents BECN2-mediated drug tolerance to cannabinoids.

Autophagy

Kuramoto K,Wang N,Fan Y,Zhang W,Schoenen FJ,Frankowski KJ,Marugan J,Zhou Y,Huang S,He C

MA5-14794 was used in immunocytochemistry to analyze prevention of BECN2-mediated drug tolerance to cannabioids by autophagy activation by novel inducers

Thu Sep 01 00:00:00 UTC 2016

Variants of Rab GTPase-Effector Binding Protein-2 Cause Variation in the Collateral Circulation and Severity of Stroke.

Stroke

Lucitti JL,Sealock R,Buckley BK,Zhang H,Xiao L,Dudley AC,Faber JE

MA514794 was used in immunohistochemistry and western blot to observe that a single amino acid change within Rabep2 accounting for the difference in collateral extent and infarct volume between B6 and BC mice maps to Dce1

Thu Dec 01 00:00:00 UTC 2016

Altered dynamics of a lipid raft associated protein in a kidney model of Fabry disease.

Molecular genetics and metabolism

Labilloy A,Youker RT,Bruns JR,Kukic I,Kiselyov K,Halfter W,Finegold D,do Monte SJ,Weisz OA

MA5-14794 was used in immunocytochemistry to use a renal epithelial cell model of Fabry disease to study the effects of globotriaosylceramide accumulation on lipid raft protein dynamics

Sat Feb 01 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: Early endosome antigen 1; early endosome antigen 1, 162kD; early endosome-associated protein; Endosome-associated protein p162; Zinc finger FYVE domain-containing protein 2

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Gene Aliases: A430109M19Rik; B230358H09Rik; EEA1; MST105; MSTP105; ZFYVE2

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UniProt ID: (Human) Q15075, (Mouse) Q8BL66

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Entrez Gene ID: (Human) 8411, (Rat) 314764, (Mouse) 216238

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Function(s)

protein binding calmodulin binding 1-phosphatidylinositol binding zinc ion binding GTP-dependent protein binding protein homodimerization activity metal ion binding

Process(es)

endocytosis vesicle fusion synaptic vesicle to endosome fusion early endosome to late endosome transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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EEA1 Monoclonal Antibody (F.43.1)

EEA1 Antibody (MA5-14794) in ICC/IF

Product Details

MA5-14794

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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