Proteintech

EED Polyclonal Antibody

EED Antibody in Immunocytochemistry (ICC/IF)

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EED Antibody (16818-1-AP) in ICC/IF

Immunofluorescent analysis of ( 10% Formalin) fixed HepG2 cells using 16818-1-AP (EED antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

EED Antibody in Immunohistochemistry (Paraffin) (IHC (P))

EED Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

16818-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 14 publications 14 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

EED Fusion Protein Ag10358 (1-466 aa encoded by BC068995)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.13 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MSEREVSTA PAGTDMPAAK KQKLSSDENS NPDLSGDEND DAVSIESGTN TERPDTPTNT PNAPGRKSWG KGKWKSKKCK YSFKCVNSLK EDHNQPLFGV QFNWHSKEGD PLVFATVGSN RVTLYECHSQ GEIRLLQSYV DADADENFYT CAWTYDSNTS HPLLAVAGSR GIIRIINPIT MQCIKHYVGH GNAINELKFH PRDPNLLLSV SKDHALRLWN IQTDTLVAIF GGVEGHRDEV LSADYDLLGE KIMSCGMDHS LKLWRINSKR MMNAIKESYD YNPNKTNRPF ISQKIHFPDF STRDIHRNYV DCVRWLGDLI LSKSGRAILH SHQQCMRDPV SPNLRQHLSC ENAIVCWKPG KMEDDIDKIK PSESNVTILG RFDYSQCDIW YMRFSMDFWQ KMLALGNQVG KLYVWDLEVE DPHKAKCTTL THHKCGAAIR QTSFSRDSSI LIAVCDDASI WRWDRLR (1-466 aa encoded by BC068995)

Target Information

The EED protein (WAIT-1 or WD protein associated with integrin cytoplasmic tails-1), also called embryonic ectoderm development, is a member of the superfamily of WD-40 repeat proteins and widely conserved polycomb group (PcG) family of proteins. The polycomb group (PcG) is a large and evolutionarily conserved set of genes whose products act in multimeric complexes to modify histones, which are then thought to cause stable and heritable states of transcriptional repression. EED shuttles between the nucleus and the plasma membrane and can interact with the cytoplasmic tail of integrin Beta-7 subunit. EED exerted an antiviral activity at the late stage of HIV-1 replication.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

METTL14 regulates chromatin bivalent domains in mouse embryonic stem cells.

Cell reports

Mu M,Li X,Dong L,Wang J,Cai Q,Hu Y,Wang D,Zhao P,Zhang L,Zhang D,Cheng S,Tan L,Wu F,Shi YG,Xu W,Shi Y,Shen H

Tue Jun 27 00:00:00 UTC 2023

Nucleolus assembly impairment leads to two-cell transcriptional repression via NPM1-mediated PRC2 recruitment.

Nature structural & molecular biology

Li D,Cao R,Li Q,Yang Y,Tang A,Zhang J,Liu Q

Sat Jul 01 00:00:00 UTC 2023

Phosphorylation and stabilization of EZH2 by DCAF1/VprBP trigger aberrant gene silencing in colon cancer.

Nature communications

Ghate NB,Kim S,Shin Y,Kim J,Doche M,Valena S,Situ A,Kim S,Rhie SK,Lenz HJ,Ulmer TS,Mumenthaler SM,An W

Mon Apr 17 00:00:00 UTC 2023

A plant-derived glucocorticoid receptor modulator with potency to attenuate the side effects of glucocorticoid therapy.

British journal of pharmacology

Wang Y,Gao J,Yu Y,Zhou L,Wang M,Xue W,Liu B,Wu X,Wu X,Gao H,Shen Y,Xu Q

Sun Jan 01 00:00:00 UTC 2023

A plant-derived glucocorticoid receptor modulator with potency to attenuate the side effects of glucocorticoid therapy.

British journal of pharmacology

Wang Y,Gao J,Yu Y,Zhou L,Wang M,Xue W,Liu B,Wu X,Wu X,Gao H,Shen Y,Xu Q

Sun Jan 01 00:00:00 UTC 2023

A plant-derived glucocorticoid receptor modulator with potency to attenuate the side effects of glucocorticoid therapy.

British journal of pharmacology

Wang Y,Gao J,Yu Y,Zhou L,Wang M,Xue W,Liu B,Wu X,Wu X,Gao H,Shen Y,Xu Q

Sun Jan 01 00:00:00 UTC 2023

A plant-derived glucocorticoid receptor modulator with potency to attenuate the side effects of glucocorticoid therapy.

British journal of pharmacology

Wang Y,Gao J,Yu Y,Zhou L,Wang M,Xue W,Liu B,Wu X,Wu X,Gao H,Shen Y,Xu Q

Sun Jan 01 00:00:00 UTC 2023

Transcriptional and H3K27ac related genome profiles in oral squamous cell carcinoma cells treated with metformin.

Journal of Cancer

Liu S,Shi C,Hou X,Tian X,Li C,Ma X,Wang X,Gao P

Wed Apr 13 00:00:00 UTC 2022

Discovery of precision targeting EZH2 degraders for triple-negative breast cancer.

European journal of medicinal chemistry

Wang C,Chen X,Liu X,Lu D,Li S,Qu L,Yin F,Luo H,Zhang Y,Luo Z,Cui N,Kong L,Wang X

Fri Aug 05 00:00:00 UTC 2022

Mechanical force-sensitive lncRNA SNHG8 inhibits osteogenic differentiation by regulating EZH2 in hPDLSCs.

Cellular signalling

Zhang Z,He Q,Yang S,Zhao X,Li X,Wei F

Sun May 01 00:00:00 UTC 2022

PRMT1-mediated EZH2 methylation promotes breast cancer cell proliferation and tumorigenesis.

Cell death & disease

Li Z,Wang D,Chen X,Wang W,Wang P,Hou P,Li M,Chu S,Qiao S,Zheng J,Bai J

Sat Nov 13 00:00:00 UTC 2021

PRMT1-mediated EZH2 methylation promotes breast cancer cell proliferation and tumorigenesis.

Cell death & disease

Li Z,Wang D,Chen X,Wang W,Wang P,Hou P,Li M,Chu S,Qiao S,Zheng J,Bai J

Sat Nov 13 00:00:00 UTC 2021

Interplay of miR-137 and EZH2 contributes to the genome-wide redistribution of H3K27me3 underlying the Pb-induced memory impairment.

Cell death & disease

Gu X,Xu Y,Xue WZ,Wu Y,Ye Z,Xiao G,Wang HL

Wed Sep 11 00:00:00 UTC 2019

Bach1 regulates self-renewal and impedes mesendodermal differentiation of human embryonic stem cells.

Science advances

Wei X,Guo J,Li Q,Jia Q,Jing Q,Li Y,Zhou B,Chen J,Gao S,Zhang X,Jia M,Niu C,Yang W,Zhi X,Wang X,Yu D,Bai L,Wang L,Na J,Zou Y,Zhang J,Zhang S,Meng D

Fri Mar 01 00:00:00 UTC 2019

Identification and Functional Characterization of a New Splicing Variant of EZH2 in the Central Nervous System.

International journal of biological sciences

Li D,Wang HL,Huang X,Gu X,Xue W,Xu Y

Mon Jan 06 00:00:00 UTC 2020

Identification and Functional Characterization of a New Splicing Variant of EZH2 in the Central Nervous System.

International journal of biological sciences

Li D,Wang HL,Huang X,Gu X,Xue W,Xu Y

Mon Jan 06 00:00:00 UTC 2020

A covalently bound inhibitor triggers EZH2 degradation through CHIP-mediated ubiquitination.

The EMBO journal

Wang X,Cao W,Zhang J,Yan M,Xu Q,Wu X,Wan L,Zhang Z,Zhang C,Qin X,Xiao M,Ye D,Liu Y,Han Z,Wang S,Mao L,Wei W,Chen W

Tue May 02 00:00:00 UTC 2017

A covalently bound inhibitor triggers EZH2 degradation through CHIP-mediated ubiquitination.

The EMBO journal

Wang X,Cao W,Zhang J,Yan M,Xu Q,Wu X,Wan L,Zhang Z,Zhang C,Qin X,Xiao M,Ye D,Liu Y,Han Z,Wang S,Mao L,Wei W,Chen W

Tue May 02 00:00:00 UTC 2017

Multiple regulatory aspects of histone methyltransferase EZH2 in Pb-induced neurotoxicity.

Oncotarget

Xue WZ,Gu X,Wu Y,Li D,Xu Y,Wang HL

Tue Oct 17 00:00:00 UTC 2017

The EZH1-SUZ12 complex positively regulates the transcription of NF-κB target genes through interaction with UXT.

Journal of cell science

Su SK,Li CY,Lei PJ,Wang X,Zhao QY,Cai Y,Wang Z,Li L,Wu M

Wed Jun 15 00:00:00 UTC 2016

The EZH1-SUZ12 complex positively regulates the transcription of NF-κB target genes through interaction with UXT.

Journal of cell science

Su SK,Li CY,Lei PJ,Wang X,Zhao QY,Cai Y,Wang Z,Li L,Wu M

Wed Jun 15 00:00:00 UTC 2016

Polycomb repressive complex 2 facilitates the nuclear export of the influenza viral genome through the interaction with M1.

Scientific reports

Asaka MN,Kawaguchi A,Sakai Y,Mori K,Nagata K

Tue Sep 20 00:00:00 UTC 2016

Polycomb repressive complex 2 facilitates the nuclear export of the influenza viral genome through the interaction with M1.

Scientific reports

Asaka MN,Kawaguchi A,Sakai Y,Mori K,Nagata K

Tue Sep 20 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: Embryonic ectoderm development protein; hEED; Polycomb protein EED; WAIT-1; WD protein associating with integrin cytoplasmic tails 1

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Gene Aliases: EED; HEED; WAIT1

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UniProt ID: (Human) O75530

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Entrez Gene ID: (Human) 8726

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Function(s)

chromatin binding protein binding histone methyltransferase activity identical protein binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter regulation of gene expression by genetic imprinting transcription, DNA-templated histone methylation spinal cord development negative regulation of gene expression, epigenetic negative regulation of transcription, DNA-templated positive regulation of histone H3-K27 methylation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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EED Polyclonal Antibody

EED Antibody (16818-1-AP) in ICC/IF

Product Details

16818-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (MISC)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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