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EEF1A1 Antibody (711669) in WB
Western blot analysis was performed on whole cell (30 µg lysate) extracts of PC-3 (Lane 1), THP-1 (Lane 2), K-562 (Lane 3), HUVEC (Lane 4), MCF-7 (Lane 5) and tissue extract of mouse muscle (Lane 6). The blots were probed with Anti-EEF1A1 Recombinant Rabbit Superclonal™ Antibody (Product # 711669, 1-2 µg/mL). A 50 kDa band corresponding to EEF1A1 was observed across the cell lines and tissue tested. The blots were detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product ... View More
Product Details
711669
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Immunogen
Peptide corresponding to human EEF1A1 [ 1) 156-172, 2) 322-341]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2692262
Product Specific Information
This antibody is predicted to react with Mouse,rat,monkey, Bovine
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. With PARP1 and TXK, forms a complex that acts as a T helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cervical cancer suppressor 3; CTCL tumor antigen; eEF1A-1; EF-1-alpha-1; EF-Tu; EF1a-like protein; EF1A1; elongation factor 1 A-1; elongation factor 1 alpha subunit; Elongation factor 1-alpha 1; Elongation factor Tu; Eukaryotic elongation factor 1 A-1; eukaryotic translation elongation factor 1 alpha 1-like 14; glucocorticoid receptor AF-1 specific elongation factor; GRAF 1EF; leukocyte receptor cluster (LRC) member 7; Leukocyte receptor cluster member 7; prostate tumor-inducing protein 1; translation elongation factor 1 alpha 1-like 14
Gene Aliases: CCS-3; CCS3; EE1A1; EEF-1; EEF1A; eEF1A-1; EEF1A1; EF-Tu; EF1A; GRAF-1EF; HNGC:16303; LENG7; PTI1
UniProt ID: (Human) P68104, (Mouse) P10126
Entrez Gene ID: (Human) 1915, (Mouse) 13627
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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