Proteintech

EEF1B2 Polyclonal Antibody

EEF1B2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 13

EEF1B2 Antibody (10483-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human pancreas cancer using 10483-1-AP (EEF1B2 antibody) at dilution of 1:100 (under 25x lens).

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Product Details

10483-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 10 publications 10 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

EEF1B2 Fusion Protein Ag0742 (1-225 aa encoded by BC004931)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.21 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody is a rabbit polyclonal antibody raised against full length EEF1B2 of human origin.

Immunogen sequence: MGFGDLKSP AGLQVLNDYL ADKSYIEGYV PSQADVAVFE AVSSPPPADL CHALRWYNHI KSYEKEKASL PGVKKALGKY GPADVEDTTG SGATDSKDDD DIDLFGSDDE EESEEAKRLR EERLAQYESK KAKKPALVAK SSILLDVKPW DDETDMAKLE ECVRSIQADG LVWGSSKLVP VGYGIKKLQI QCVVEDDKVG TDMLEEQITA FEDYVQSMDV AAFNKI (1-225 aa encoded by B C004931)

Target Information

EF-1 (elongation factor-1) is a multi-protein complex that is responsible forthe delivery of aminoacyl-tRNAs to the ribosome. The EF-1 protein complexis composed of four subunits: EF-1 α, EF-1 β, EF-1 δ and EF-1 γ. EF-1 β is a225 amino acid member of the EF-1 β/EF-1 δ protein family and contains oneGST C-terminal domain. Both EF-1 β and EF-1 δ stimulate the exchange ofGDP bound to EF-1 α to GTP. Phosphorylation of EF-1 β affects the GDP/GTP exchange rate. Alternative splicing events of the gene that encodes EF-1 β result in three transcript variants that differ only in the 5' UTR.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Autism- and epilepsy-associated EEF1A2 mutations lead to translational dysfunction and altered actin bundling.

bioRxiv : the preprint server for biology

Mohamed MS,Klann E

Wed Jun 07 00:00:00 UTC 2023

Identification and Validation of Novel Potential Pathogenesis and Biomarkers to Predict the Neurological Outcome after Cardiac Arrest.

Brain sciences

Zhang Q,Zhang C,Liu C,Zhan H,Li B,Lu Y,Wei H,Cheng J,Li S,Wang C,Hu C,Liao X

Fri Jul 15 00:00:00 UTC 2022

Dual genome-wide CRISPR knockout and CRISPR activation screens identify mechanisms that regulate the resistance to multiple ATR inhibitors.

PLoS genetics

Schleicher EM,Dhoonmoon A,Jackson LM,Clements KE,Stump CL,Nicolae CM,Moldovan GL

Sun Nov 01 00:00:00 UTC 2020

Heat Shock-Induced Dephosphorylation of Eukaryotic Elongation Factor 1BδL by Protein Phosphatase 1.

Frontiers in molecular biosciences

Kaitsuka T,Tomizawa K,Matsushita M

Tue Feb 02 00:00:00 UTC 2021

Deficiency of TPPP2, a factor linked to oligoasthenozoospermia, causes subfertility in male mice.

Journal of cellular and molecular medicine

Zhu F,Yan P,Zhang J,Cui Y,Zheng M,Cheng Y,Guo Y,Yang X,Guo X,Zhu H

Mon Apr 01 00:00:00 UTC 2019

Polyamine stimulation of eEF1A synthesis based on the unusual position of a complementary sequence to 18S rRNA in eEF1A mRNA.

Amino acids

Terui Y,Sakamoto A,Yoshida T,Kasahara T,Tomitori H,Higashi K,Igarashi K,Kashiwagi K

Sun Feb 01 00:00:00 UTC 2015

Polyamine stimulation of eEF1A synthesis based on the unusual position of a complementary sequence to 18S rRNA in eEF1A mRNA.

Amino acids

Terui Y,Sakamoto A,Yoshida T,Kasahara T,Tomitori H,Higashi K,Igarashi K,Kashiwagi K

Sun Feb 01 00:00:00 UTC 2015

Prenatal alcohol exposure alters phosphorylation and glycosylation of proteins in rat offspring liver.

Proteomics

Fofana B,Yao XH,Rampitsch C,Cloutier S,Wilkins JA,Nyomba BL

Mon Feb 01 00:00:00 UTC 2010

Prenatal alcohol exposure alters phosphorylation and glycosylation of proteins in rat offspring liver.

Proteomics

Fofana B,Yao XH,Rampitsch C,Cloutier S,Wilkins JA,Nyomba BL

Mon Feb 01 00:00:00 UTC 2010

Integrated proteomic analysis of human cancer cells and plasma from tumor bearing mice for ovarian cancer biomarker discovery.

PloS one

Pitteri SJ,JeBailey L,Faça VM,Thorpe JD,Silva MA,Ireton RC,Horton MB,Wang H,Pruitt LC,Zhang Q,Cheng KH,Urban N,Hanash SM,Dinulescu DM

Thu Nov 19 00:00:00 UTC 2009

Integrated proteomic analysis of human cancer cells and plasma from tumor bearing mice for ovarian cancer biomarker discovery.

PloS one

Pitteri SJ,JeBailey L,Faça VM,Thorpe JD,Silva MA,Ireton RC,Horton MB,Wang H,Pruitt LC,Zhang Q,Cheng KH,Urban N,Hanash SM,Dinulescu DM

Thu Nov 19 00:00:00 UTC 2009

Integrated proteomic analysis of human cancer cells and plasma from tumor bearing mice for ovarian cancer biomarker discovery.

PloS one

Pitteri SJ,JeBailey L,Faça VM,Thorpe JD,Silva MA,Ireton RC,Horton MB,Wang H,Pruitt LC,Zhang Q,Cheng KH,Urban N,Hanash SM,Dinulescu DM

Thu Nov 19 00:00:00 UTC 2009

Translational repression by RNA-binding protein TIAR.