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EFHD2 Antibody (PA5-78575) in ICC/IF
EFHD2 Polyclonal Antibody detects EFHD2 protein by immunofluorescent analysis. Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: EFHD2 protein stained by EFHD2 Polyclonal Antibody (Product # PA5-78575) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta-3 Tubulin Polyclonal Antibody [GT1338]diluted at 1:500. Blue: Fluoroshield with DAPI .
Product Details
PA5-78575
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human EFHD2. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2735756
Product Specific Information
Positive Control: H1299, HeLaS3, mouse brain, rat brain
Predicted Reactivity: Rhesus Monkey (100%), Bovine (100%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
EFHD2, also known as Swiprosin-1 or SWS1, is an EF-hand and coiled-coil-containing adaptor protein that plays a role in lymphocyte physiology. EFHD2 exhibits the highest expression in CD8+ T cells and immature B cells. It provides a membrane scaffold that is required for the Syk-, SLP-65-, and PLC-gamma2-dependent B-cell receptor (BCR)-induced calcium flux. EFHD2 may also regulate BCR-induced immature and primary B-cell apoptosis. It controls spontaneous apoptosis through the regulation of BCL2L1 abundance. Also, EFHD2 plays a role as negative regulator of the canonical NF-kappa-B-activating branch.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EF hand domain containing 2; EF hand domain family, member D2; EF-hand domain family, member D2; EF-hand domain-containing protein D2; MGC4342; RP3-467K16.3; swiprosin 1; Swiprosin-1; testicular tissue protein Li 62
Gene Aliases: 2600015J22Rik; AA408606; D4Wsu27e; EFHD2; SWS1
UniProt ID: (Human) Q96C19, (Rat) Q4FZY0
Entrez Gene ID: (Human) 79180, (Mouse) 27984, (Rat) 298609
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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