Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Product Details
PA5-79187
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived mouse EGF recombinant protein (Position: N977-R1029).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2746303
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: mouse kidney tissue.
Target Information
EGF (Epidermal growth factor) exerts its actions by binding to the EGF Receptor, a 170 kDa protein kinase. Activation of EGFR initiates diverse cellular pathways in response to toxic environmental stimuli, or to EGF binding to the receptor, the EGFR forms homo- or heterodimers with other family members. Each dimeric receptor complex initiates a distinct signaling pathway by recruiting different Src homology 2 (SH2) containing effector proteins. EGF is far and wide expressed in kidney, cerebrum, prostrate and salivary glands. EGF acts as a potent mitogenic factor and the phosphorylated receptor recruits adapter proteins like GRB2 that activates complex downstream signaling cascades. EGF activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STAT modules. Research studies suggest the protein may also play important role in activating the NF-kappa-B signaling cascade. Defects in the EGGF gene are the cause of hypomagnesemia type 4 and dysregulation has been associated with the growth and progression of certain cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: EGF; H-EGF; Pro-epidermal growth factor; Pro-epidermal growth factor precursor (EGF)
Gene Aliases: AI790464; Egf
UniProt ID: (Mouse) P01132
Entrez Gene ID: (Mouse) 13645
activation of MAPKK activity
angiogenesis
epidermal growth factor receptor signaling pathway
STAT protein import into nucleus
positive regulation of cell proliferation
positive regulation of peptidyl-threonine phosphorylation
peptidyl-tyrosine phosphorylation
positive regulation of cerebellar granule cell precursor proliferation
positive regulation of catenin import into nucleus
positive regulation of phosphorylation
positive regulation of DNA binding
positive regulation of MAP kinase activity
positive regulation of epidermal growth factor-activated receptor activity
positive regulation of mitotic nuclear division
positive regulation of transcription, DNA-templated
branching morphogenesis of an epithelial tube
regulation of protein secretion
regulation of peptidyl-tyrosine phosphorylation
negative regulation of secretion
regulation of protein transport
mammary gland alveolus development
ERK1 and ERK2 cascade
regulation of calcium ion import
negative regulation of cholesterol efflux
positive regulation of hyaluronan biosynthetic process
regulation of protein localization to cell surface
positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support