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EGFL5 Antibody (PA5-106685) in ICC/IF
Immunocytochemistry analysis of EGFL5 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with EGFL5 polyclonal antibody (Product # PA5-106685) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-106685
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human MEGF9(Accession Q9H1U4), corresponding to amino acid residues C383-W433.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2854353
Product Specific Information
Antibody detects endogenous levels of total MEGF9.
Target Information
MEGF9 is a protein coding gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EGF-like protein 5; EGF-like-domain, multiple 5; Epidermal growth factor-like protein 5; Multiple EGF-like domains protein 9; multiple EGF-like-domains 9; Multiple epidermal growth factor-like domains protein 9
Gene Aliases: 4933405H16Rik; 9630025C07; EGFL5; KIAA0818; MEGF9; mKIAA0818; UNQ671/PRO1305
UniProt ID: (Human) Q9H1U4, (Mouse) Q8BH27
Entrez Gene ID: (Human) 1955, (Rat) 313270, (Mouse) 230316
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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