Invitrogen

EGFR Antibody Cocktail

EGFR Antibody in Immunocytochemistry (ICC/IF)

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EGFR Antibody (MA5-13343) in ICC/IF

Immunofluorescence analysis of EGFR was done on 90% confluent log phase A431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled EGFR (111.6+199.12+ F4+11E8) Mouse Monoclonal Antibody (Product # MA5-13343) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A... View More

EGFR Antibody in Immunoprecipitation (IP)

Product Image Gallery

EGFR Antibody — EGF Receptor (EGFR) Protein Schematic. EGF Receptor (EGFR) Protein Schematic-Epidermal Growth Factor Receptor, (EGFR, a 185 kDa glycoprotein), also known as erbB, is a transmembrane tyrosine kinase that regulates a variety of biological responses ranging from mitogenesis to stress signaling. The EGFR consists of a large extracellular domain, a single transmembrane domain and a cytoplasmic domain that exhibits kinase activity. Upon binding of EGF to the extracellular domain, the receptor undergoes dimerization and becomes phosphorylated on several tyrosine residues within the cytoplasmic domain. These events result in EGFR activation and increased tyrosine kinase activity toward a variety of intracellular substrates.

Product Details

MA5-13343

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 8 publications 8 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

2 µg/mL

View 2 publications 2 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

Immunoprecipitation (IP)

2 µg/mL

View 3 publications 3 publications

Product Specifications

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1/IgG2a

Class

Cocktail

Type

Antibody

Clone

111.6, 199.12, F4, 11E8

Immunogen

Extracellular and cytoplasmic domains of recombinant human EGFR protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Storage conditions

4°C

Shipping conditions

Wet ice

AB_10984241

Product Specific Information

MA5-13343 targets Epidermal Growth Factor Receptor in IP and WB applications and shows reactivity with Human samples.

The MA5-13343 immunogen is extracellular and cytoplasmic domains of recombinant human EGFR protein.

This cocktail is also referred to as clone R19/48.

Target Information

EGFR, epidermal growth factor receptor, is a receptor tyrosine kinases that signals in response to various growth factors. Overexpression has been linked to numerous types of cancer and EGFR is the target of both biological and small molecular therapeutics. EGFR is encoded by the EGFR gene located on chromosome 7 in humans. EGFR belongs to the HER/ERbB family of proteins that includes three other receptor tyrosine kinases, ERbB2, ERbB3, ERbB4. EGFR is a transmembrane receptor and binding of its cognate ligands such as EGF (Epidermal Growth Factor) and TGF alpha (Transforming Growth Factor alpha) to the extracellular domain leads to EGFR dimerization followed by autophosphorylation of the tyrosine residues in the cytoplasmic domain. Overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Interleukin-13 receptor alpha 2 cooperates with EGFRvIII signaling to promote glioblastoma multiforme.

Nature communications

Newman JP,Wang GY,Arima K,Guan SP,Waters MR,Cavenee WK,Pan E,Aliwarga E,Chong ST,Kok CYL,Endaya BB,Habib AA,Horibe T,Ng WH,Ho IAW,Hui KM,Kordula T,Lam PYP

MA5-13343 was used in Immunohistochemistry to characterise how IL-13Rα acts as a molecular switch between invasion and ptoliferation, cooperating with EGFRvIII signalling to promote glioblastoma multiforme.

Mon Dec 04 00:00:00 UTC 2017

Cancer cells exploit eIF4E2-directed synthesis of hypoxia response proteins to drive tumor progression.

Cancer research

Uniacke J,Perera JK,Lachance G,Francisco CB,Lee S

MA5-13343 was used in western blot to study the role of eIF4E2 in the ability of tumors to survive and proliferate under conditions of hypoxia

Sat Mar 01 00:00:00 UTC 2014

Facile chemical functionalization of proteins through intein-linked yeast display.

Bioconjugate chemistry

Marshall CJ,Agarwal N,Kalia J,Grosskopf VA,McGrath NA,Abbott NL,Raines RT,Shusta EV

MA5-13343 was used in flow cytometry to develop an intein-based yeast display system to enable the simple chemical functionalization of proteins

Wed Sep 18 00:00:00 UTC 2013

Regulation of EGFR trafficking and cell signaling by Sprouty2 and MIG6 in lung cancer cells.

Journal of cell science

Walsh AM,Lazzara MJ

MA5-13343 was used in immunoprecipitation to study the role of Sprouty2 and MIG6 in modulating lung cancer cell EGFR trafficking and signaling

Tue Oct 01 00:00:00 UTC 2013

Metastasis suppressor tetraspanin CD82/KAI1 regulates ubiquitylation of epidermal growth factor receptor.

The Journal of biological chemistry

Odintsova E,van Niel G,Conjeaud H,Raposo G,Iwamoto R,Mekada E,Berditchevski F

MA5-13343 was used in immunoprecipitation to study the modulation of EGFR ubiquitinylation by the CD82 metastasis suppressor

Fri Sep 06 00:00:00 UTC 2013

An oxygen-regulated switch in the protein synthesis machinery.

Nature

Uniacke J,Holterman CE,Lachance G,Franovic A,Jacob MD,Fabian MR,Payette J,Holcik M,Pause A,Lee S

MA5-13343 was used in western blot to study an oxygen-regulated translation initiation complex

Sun May 06 00:00:00 UTC 2012

Ubiquitin-dependent regulation of COPII coat size and function.

Nature

Jin L,Pahuja KB,Wickliffe KE,Gorur A,Baumgärtel C,Schekman R,Rape M

MA5-13343 was used in immunocytochemistry to study the modulation of COPII coat size and function by ubiquitin ligase CUL3-KLHL12

Wed Feb 22 00:00:00 UTC 2012

Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.

Molecular & cellular proteomics : MCP

Park JE,Tan HS,Datta A,Lai RC,Zhang H,Meng W,Lim SK,Sze SK

MA5-13343 was used in western blot to investigate the mechanism of angiogenesis and metastasis promoted by hypoxic tumor cells

Tue Jun 01 00:00:00 UTC 2010

Structure-function analysis of nucleolin and ErbB receptors interactions.

PloS one

Farin K,Di Segni A,Mor A,Pinkas-Kramarski R

MA5-13343 was used in western blot to investigate the interaction between nucleolin and ErbB receptors

Fri Jul 03 00:00:00 UTC 2009

MMTV-EGF receptor transgene promotes preneoplastic conversion of multiple steroid hormone-responsive tissues.

Journal of cellular biochemistry

Marozkina NV,Stiefel SM,Frierson HF,Parsons SJ

MA5-13343 was used in western blot to investigate the function of epidermal growth factor receptor in the initiation of reproductive tissue tumor development

Tue Apr 15 00:00:00 UTC 2008

Epidermal growth factor ligand/receptor loop and downstream signaling activation pattern in completely resected nonsmall cell lung cancer.

Cancer

Volante M,Saviozzi S,Rapa I,Ceppi P,Cappia S,Calogero R,Novello S,Borasio P,Papotti M,Scagliotti GV

MA5-13343 was used in immunohistochemistry to study EGF receptor pathway activation in completely resected non-small cell lung cancer

Sat Sep 15 00:00:00 UTC 2007

Surface charge and hydrophobicity determine ErbB2 binding to the Hsp90 chaperone complex.

Nature structural & molecular biology

Xu W,Yuan X,Xiang Z,Mimnaugh E,Marcu M,Neckers L

MA5-13343 was used in western blot to study the role of surface charge and hydrophobicity in ErbB2 binding to the Hsp90 chaperone complex

Tue Feb 01 00:00:00 UTC 2005

Sulindac metabolites inhibit epidermal growth factor receptor activation and expression.

Journal of carcinogenesis

Pangburn HA,Kraus H,Ahnen DJ,Rice PL

MA5-13343 was used in western blot to test if sulindac metabolites block activation or expression of the epidermal growth factor receptor

Fri Sep 02 00:00:00 UTC 2005

Racial disparity of epidermal growth factor receptor expression in prostate cancer.

Journal of clinical oncology : official journal of the American Society of Clinical Oncology

Shuch B,Mikhail M,Satagopan J,Lee P,Yee H,Chang C,Cordon-Cardo C,Taneja SS,Osman I

MA5-13343 was used in immunohistochemistry to study racial differences in EGFR expression in prostate cancer

Wed Dec 01 00:00:00 UTC 2004

In vitro chemoresistance and biomarker profiles are unique for histologic subtypes of epithelial ovarian cancer.

Gynecologic oncology

Cloven NG,Kyshtoobayeva A,Burger RA,Yu IR,Fruehauf JP

MA5-13343 was used in immunohistochemistry to study whether there is a relationship between histologic subtype of epithelial ovarian cancer and chemoresistance

Thu Jan 01 00:00:00 UTC 2004

Characterization of a transplantable hormone-responsive human prostatic cancer xenograft TEN12 and its androgen-resistant sublines.

The Prostate

Harper ME,Goddard L,Smith C,Nicholson RI

MA5-13343 was used in immunocytochemistry to investigate the effectiveness of Xenograft TEN12 and its sublines on the progression of prostatic cancer

Thu Jan 01 00:00:00 UTC 2004

Autocrine extracellular signal-regulated kinase (ERK) activation in normal human keratinocytes: metalloproteinase-mediated release of amphiregulin triggers signaling from ErbB1 to ERK.

Molecular biology of the cell

Kansra S,Stoll SW,Johnson JL,Elder JT

MA5-13343 was used in immunoprecipitation to investigate the mechanism of ERK autocrine activation in normal human keratinocytes

Wed Sep 01 00:00:00 UTC 2004

Neuregulin expression, function, and signaling in human ovarian cancer cells.

Clinical cancer research : an official journal of the American Association for Cancer Research

Gilmour LM,Macleod KG,McCaig A,Sewell JM,Gullick WJ,Smyth JF,Langdon SP

MA5-13343 was used in western blot to investigate the characteristics of neuregulin in human ovarian cancer cells

Sun Dec 01 00:00:00 UTC 2002

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