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Invitrogen
EGLN1/EGLN2 Polyclonal Antibody
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FIGURE: 1 / 1
EGLN1/EGLN2 Antibody (PA5-109677) in WB
Western blot analysis of EGLN1/EGLN2 in extracts of various cell lines. Samples were incubated with EGLN1/EGLN2 Polyclonal antibody (Product # PA5-109677) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-109677
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 250-350 of human EGLN1/EGLN2 (NP_0713341/NP_4442741)
Conjugate
Form
Liquid
Concentration
0.81 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2855088
Product Specific Information
Immunogen sequence: DIRGDKITWI EGKEPGCETI GLLMSSMDDL IRHCNGKLGS YKINGRTKAM VACYPGNGTG YVRHVDNPNG DGRCVTCIYY LNKDWDAKVS GGILRIFPEG K
Target Information
Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF1B. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN1 is the most important isozyme under normoxia and, through regulating the stability of HIF1, involved in various hypoxia-influenced processes such as angiogenesis in retinal and cardiac functionality. Target proteins are preferentially recognized via a LXXLAP motif.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Egl nine homolog 1; Egl nine homolog 2; egl nine-like protein 1; egl-9 family hypoxia-inducible factor 1; egl-9 family hypoxia-inducible factor 2; EIT-6; Estrogen-induced tag 6; HIF prolyl hydroxylase 2; HIF-PH1; HIF-PH2; HIF-prolyl hydroxylase 1; HIF-prolyl hydroxylase 2; HPH-3; Hypoxia-inducible factor prolyl hydroxylase 1; Hypoxia-inducible factor prolyl hydroxylase 2; PHD1; PHD2; Prolyl hydroxylase domain-containing protein 1; Prolyl hydroxylase domain-containing protein 2; Prolyl hydroxylase EGLN2; SM-20; zinc finger MYND domain-containing protein 6
Gene Aliases: C1orf12; ECYT3; EGLN1; EGLN2; EIT6; HALAH; HIF-PH1; HIF-PH2; HIFPH1; HIFPH2; HPH-1; HPH-2; HPH-3; HPH2; PHD1; PHD2; PNAS-118; PNAS-137; SM20; ZMYND6
UniProt ID: (Human) Q9GZT9, (Human) Q96KS0
Entrez Gene ID: (Human) 54583, (Human) 112398
iron ion binding
protein binding
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
enzyme binding
L-ascorbic acid binding
peptidyl-proline dioxygenase activity
peptidyl-proline 4-dioxygenase activity
ferrous iron binding
oxygen sensor activity
regulation of cell growth
response to hypoxia
peptidyl-proline hydroxylation to 4-hydroxy-L-proline
intracellular estrogen receptor signaling pathway
regulation of neuron apoptotic process
cell redox homeostasis
positive regulation of protein catabolic process
oxidation-reduction process
regulation of transcription from RNA polymerase II promoter in response to hypoxia
negative regulation of cAMP catabolic process
oxygen homeostasis
negative regulation of sequence-specific DNA binding transcription factor activity
regulation of angiogenesis
negative regulation of cyclic-nucleotide phosphodiesterase activity
cardiac muscle tissue morphogenesis
heart trabecula formation
ventricular septum morphogenesis
labyrinthine layer development
response to nitric oxide
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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