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Invitrogen
EGR1 Monoclonal Antibody (HEGR1DS), PE, eBioscience™
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FIGURE: 1 / 1
EGR1 Antibody (12-9851-42) in Flow
Normal human peripheral blood cells were stimulated with Anti-Human CD3 Functional Grade Purified (Product # 16-0037-81) for 2 hours. Cells were then intracellularly stained with Anti-Human CD4 APC (Product # 17-0047-42) and Rat IgG2a K Isotype Control PE (Product # 12-4321-80) (left) or Anti-Human Egr1 PE (right), using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Cells in the lymphocyte gate were used for analysis.
Product Details
12-9851-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
HEGR1DS
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2572740
Product Specific Information
Description: The monoclonal antibody HEGR1DS recognizes human Egr1 (Early Growth Response 1), a C2H2-type zinc-finger transcription factor broadly expressed in various types of tissues. Egr1 is induced within minutes after cell activation and it regulates expression of numerous factors involved in cell division, growth, and differentiation. It has been shown to suppress tumorigenesis by inducing apoptosis of cancer cells but it can also promote their proliferation. Egr1 is often used as an early cell activation marker of T and B lymphocytes as well as myeloid cells.
Applications Reported: This HEGR1DS antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This HEGR1DS antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to BestProtocols®: Protocol B: One step protocol for (nuclear) intracellular proteins located under the Resources Tab online. The use of the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol is not recommended. This antibody can be used at 5 µL (0.03 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The protein encoded by this gene belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis. Studies suggest this is a cancer suppresor gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AT225; Early growth response protein 1; EGR-1; Nerve growth factor-induced protein A; NGFI-A; Transcription factor ETR103; Transcription factor Zif268; Zinc finger protein 225; Zinc finger protein Krox-24
Gene Aliases: AT225; EGR1; G0S30; KROX-24; KROX24; NGFI-A; TIS8; ZIF-268; ZNF225
UniProt ID: (Human) P18146
Entrez Gene ID: (Human) 1958
transcription regulatory region sequence-specific DNA binding
RNA polymerase II regulatory region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
histone acetyltransferase binding
sequence-specific DNA binding
metal ion binding
negative regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
response to glucose
T cell differentiation
BMP signaling pathway
response to insulin
regulation of protein sumoylation
skeletal muscle cell differentiation
regulation of apoptotic process
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
type I interferon signaling pathway
regulation of transcription from RNA polymerase II promoter in response to hypoxia
interleukin-1-mediated signaling pathway
cellular response to gamma radiation
cellular response to heparin
cellular response to mycophenolic acid
glomerular mesangial cell proliferation
positive regulation of glomerular metanephric mesangial cell proliferation
negative regulation of canonical Wnt signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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