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Invitrogen
EHD1 Recombinant Rabbit Monoclonal Antibody (5S4P9)
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EHD1 Antibody (MA5-42814) in IHC (P)
Immunohistochemistry analysis of EHD1 in paraffin-embedded human appendix. Samples were incubated with EHD1 Monoclonal antibody (Product # MA5-42814) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Product Details
MA5-42814
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
5S4P9
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human EHD1 (Q9H4M9).
Conjugate
Form
Liquid
Concentration
1.28 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2911955
Product Specific Information
Positive test controls include: HeLa, THP-1, U-251MG, Mouse liver, Mouse brain, Mouse spleen, Rat lung, Rat testis, Rat kidney. The target is usually found in the following locations: Cell membrane, Cell projection, Cytoplasmic side, Early endosome membrane, Peripheral membrane protein, Recycling endosome membrane, cilium membrane.
Immunogen sequence: MFSWVSKDAR RKKEPELFQT VAEGLRQLYA QKLLPLEEHY RFHEFHSPAL EDADFDNKPM VLLVGQYSTG KTTFIRHLIE QDFPGMRIGP EPTTDSFIAV
Target Information
EHD1 acts in early endocytic membrane fusion and membrane trafficking of recycling endosomes. EHD1 also plays a role in myoblast fusion and is recruited to endosomal membranes upon nerve growth factor stimulation, indirectly regulates neurite outgrowth. Further, EHD1 belongs to a highly conserved gene family encoding EPS15 homology (EH) domain-containing proteins. The protein-binding EH domain was first noted in EPS15, a substrate for the epidermal growth factor receptor. The EH domain has been shown to be an important motif in proteins involved in protein-protein interactions and in intracellular sorting. The EHD1 protein is thought to play a role in the endocytosis of IGF1 receptors. Alternatively spliced transcript variants have been found for the EHD1 gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: EH domain-containing protein 1; EH-domain containing 1; hPAST1; mPAST1; PAST homolog 1; Testilin
Gene Aliases: AA409636; CDABP0131; EHD1; H-PAST; HPAST1; PAST; PAST1; RGD1306960; RME-1
UniProt ID: (Human) Q9H4M9, (Mouse) Q9WVK4, (Rat) Q641Z6
Entrez Gene ID: (Human) 10938, (Mouse) 13660, (Rat) 293692
intracellular protein transport
endocytosis
positive regulation of cholesterol storage
endosomal transport
neuron projection development
endocytic recycling
low-density lipoprotein particle clearance
cholesterol homeostasis
protein homooligomerization
positive regulation of myoblast fusion
cellular response to nerve growth factor stimulus
positive regulation of endocytic recycling
blood coagulation
cilium assembly
protein localization to cilium
cell-cell adhesion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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