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Invitrogen
EHD3 Polyclonal Antibody
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FIGURE: 1 / 13
EHD3 Antibody (PA5-143924) in ICC/IF
Immunocytochemistry analysis of EHD3 in MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in EHD3 Polyclonal antibody (Product # PA5-143924) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate fo... View More
Product Details
PA5-143924
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence of human EHD3 (NELPAHLLPPSKRKVAE).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3075138
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human placenta tissue, human A549 whole cell, human Hela whole cell, human CCRF-CEM whole cell, human U-87MG whole cell, human SY-SY5Y whole cell, monkey heart tissue, rat heart tissue, rat brain tissue, rat kidney tissue, rat liver tissue, mouse heart tissue, mouse brain tissue, mouse kidney tissue, mouse liver tissue. IHC: mouse brain tissue, human ovarian serous adenocarcinoma tissue, human ovarian serous adenocarcinoma tissue, human renal clear cell carcinoma tissue, human breast cancer tissue, human lung cancer tissue, human rectal cancer tissue, human gastric adenocarcinoma tissue, rat brain tissue. ICC/IF: MCF-7 cell. Flow: MCF-7 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
The EHD3 gene encodes the EH-domain containing protein 3, part of the EHD protein family involved in the regulation of endocytic recycling and membrane trafficking. EHD3 plays a crucial role in the endosomal transport system, impacting how receptors and other membrane proteins are recycled back to the cell surface after internalization. This protein aids in maintaining cellular homeostasis by ensuring the efficient sorting and transport of cargo through the endosomal system. EHD3 has been implicated in various cellular functions, including insulin signaling, receptor recycling, and neuronal development, and significantly influences synaptic transmission and plasticity. Alterations in EHD3 expression or function can disrupt endosomal trafficking processes and are linked to conditions such as neurodegenerative diseases and cancer. Research on EHD3 is focused on uncovering its role in membrane dynamics and identifying potential therapeutic strategies for disorders involving dysfunctional endocytic pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EH domain-containing protein 3; EH-domain containing 3; EH-domain containing protein 2; PAST homolog 3
Gene Aliases: EHD2; EHD3; PAST3
UniProt ID: (Human) Q9NZN3, (Rat) Q8R491, (Mouse) Q9QXY6
Entrez Gene ID: (Human) 30845, (Rat) 192249, (Mouse) 57440
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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