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EIF2AK1 Polyclonal Antibody

EIF2AK1 Antibody in Immunocytochemistry (ICC/IF)

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EIF2AK1 Antibody (20499-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells, using EIF2AK1 antibody 20499-1-AP at 1:25 dilution and FITC- labeled goat anti-Rabbit IGG (green).

EIF2AK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

20499-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

Immunoprecipitation (IP)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

EIF2AK1 Fusion Protein Ag14236 (281-630 aa encoded by BC006524)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.36 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: FAEPTPEKE KRFGESDTEN QNNKSVKYTT NLVIRESGEL ESTLELQENG LAGLSASSIV EQQLPLRRNS HLEESFTSTE ESSEENVNFL GQTEAQYHLM LHIQMQLCEL SLWDWIVERN KRGREYVDES ACPYVMANVA TKIFQELVEG VFYIHNMGIV HRDLKPRNIF LHGPDQQVKI GDFGLACTDI LQKNTDWTNR NGKRTPTHTS RVGTCLYASP EQLEGSEYDA KSDMYSLGVV LLELFQPFGT EMERAEVLTG LRTGQLPESL RKRCPVQAKY IQHLTRRNSS QRPSAIQLLQ SELFQNSGNV NLTLQMKIIE QEKEIAELKK QLNLLSQDKG VRDDGKDGGV G (281-630 aa encoded by BC006524)

Target Information

EIF2AK1 (HRI) is one of 4 kinases that specifically phosphorylate Ser51 of translation initiation factor eIF2-alpha in response to various environmental stresses, leading to a decrease in protein sythesis. In the case of EIF2AK1, signaling is initiated by low levels of heme. The HRI gene is localized to 7p22 where its 3' end slightly overlaps the 3' end of the gene JTV1. The two genes are transcribed from opposite strands and kinase activity is induced by low levels of heme and inhibited by the presence of heme.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A mitochondrial iron-responsive pathway regulated by DELE1.

Molecular cell

Sekine Y,Houston R,Eckl EM,Fessler E,Narendra DP,Jae LT,Sekine S

Thu Jun 15 00:00:00 UTC 2023

DELE1 tracks perturbed protein import and processing in human mitochondria.

Nature communications

Fessler E,Krumwiede L,Jae LT

Wed Apr 06 00:00:00 UTC 2022

DELE1 tracks perturbed protein import and processing in human mitochondria.

Nature communications

Fessler E,Krumwiede L,Jae LT

Wed Apr 06 00:00:00 UTC 2022

Viperin triggers ribosome collision-dependent translation inhibition to restrict viral replication.

Molecular cell

Hsu JC,Laurent-Rolle M,Pawlak JB,Xia H,Kunte A,Hee JS,Lim J,Harris LD,Wood JM,Evans GB,Shi PY,Grove TL,Almo SC,Cresswell P

Thu May 05 00:00:00 UTC 2022

Cytoplasmic proteotoxicity regulates HRI-dependent phosphorylation of eIF2α via the Hsp70-Bag3 module.

iScience

Patel S,Kumar S,Baldan S,Hesin A,Yaglom J,Sherman MY

Fri May 20 00:00:00 UTC 2022

Sublethal cytochrome c release generates drug-tolerant persister cells.

Cell

Kalkavan H,Chen MJ,Crawford JC,Quarato G,Fitzgerald P,Tait SWG,Goding CR,Green DR

Thu Sep 01 00:00:00 UTC 2022

Sublethal cytochrome c release generates drug-tolerant persister cells.

Cell

Kalkavan H,Chen MJ,Crawford JC,Quarato G,Fitzgerald P,Tait SWG,Goding CR,Green DR

Thu Sep 01 00:00:00 UTC 2022

Human NLRP1 is a sensor for double-stranded RNA.

Science (New York, N.Y.)

Bauernfried S,Scherr MJ,Pichlmair A,Duderstadt KE,Hornung V

Fri Jan 29 00:00:00 UTC 2021

A pathway coordinated by DELE1 relays mitochondrial stress to the cytosol.

Nature

Fessler E,Eckl EM,Schmitt S,Mancilla IA,Meyer-Bender MF,Hanf M,Philippou-Massier J,Krebs S,Zischka H,Jae LT

Sun Mar 01 00:00:00 UTC 2020

A pathway coordinated by DELE1 relays mitochondrial stress to the cytosol.

Nature

Fessler E,Eckl EM,Schmitt S,Mancilla IA,Meyer-Bender MF,Hanf M,Philippou-Massier J,Krebs S,Zischka H,Jae LT

Sun Mar 01 00:00:00 UTC 2020

Integration of Hippo signalling and the unfolded protein response to restrain liver overgrowth and tumorigenesis.

Nature communications

Wu H,Wei L,Fan F,Ji S,Zhang S,Geng J,Hong L,Fan X,Chen Q,Tian J,Jiang M,Sun X,Jin C,Yin ZY,Liu Q,Zhang J,Qin F,Lin KH,Yu JS,Deng X,Wang HR,Zhao B,Johnson RL,Chen L,Zhou D

Thu Feb 19 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: Eukaryotic translation initiation factor 2-alpha kinase 1; HCR; he; heme regulated initiation factor 2 alpha kinase; heme sensitive initiation factor 2a kinase; Heme-controlled repressor; Heme-regulated eukaryotic initiation factor eIF-2-alpha kinase; Heme-regulated inhibitor; heme-regulated initiation factor 2-alpha kinase; heme-regulated repressor; Hemin-sensitive initiation factor 2-alpha kinase; hHRI

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Gene Aliases: EIF2AK1; HCR; HRI; KIAA1369; PRO1362

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UniProt ID: (Human) Q9BQI3

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Entrez Gene ID: (Human) 27102

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Function(s)

eukaryotic translation initiation factor 2alpha kinase activity ATP binding heme binding protein homodimerization activity

Process(es)

acute inflammatory response phagocytosis response to stress negative regulation of cell proliferation response to external stimulus regulation of translational initiation by eIF2 alpha phosphorylation regulation of eIF2 alpha phosphorylation by heme macrophage differentiation negative regulation of translational initiation by iron protoporphyrinogen IX metabolic process protein autophosphorylation negative regulation of hemoglobin biosynthetic process iron ion homeostasis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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EIF2AK1 Polyclonal Antibody

EIF2AK1 Antibody (20499-1-AP) in ICC/IF

Product Details

20499-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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