Proteintech

EIF2AK2/PKR Polyclonal Antibody

EIF2AK2/PKR Antibody in Immunocytochemistry (ICC/IF)

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EIF2AK2/PKR Antibody (18244-1-AP) in ICC/IF

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EIF2AK2, PKR antibody (Product # 18244-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

EIF2AK2/PKR Antibody in Immunohistochemistry (Paraffin) (IHC (P))

EIF2AK2/PKR Antibody in Western Blot (WB)

EIF2AK2/PKR Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

18244-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 24 publications 24 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:10-1:100

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

RNA Immunoprecipitation (RIP)

View 1 publication 1 publication

Not applicable (N/A)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

EIF2AK2, PKR Fusion Protein Ag13010 (1-373 aa encoded by BC093676)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.21 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody is specific to EIF2AK2.

Immunogen sequence: MAGDLSAGF FMEELNTYRQ KQGVVLKYQE LPNSGPPHDR RFTFQVIIDG REFPEGEGRS KKEAKNAAAK LAVEILNKEK KAVSPLLLTT TNSSEGLSMG NYIGLINRIA QKKRLTVNYE QCASGVHGPE GFHYKCKMGQ KEYSIGTGST KQEAKQLAAK LAYLQILSEE TSVKSDYLSS GSFATTCESQ SNSLVTSTLA SESSSEGDFS ADTSEINSNS DSLNSSSLLM NGLRNNQRKA KRSLAPRFDL PDMKETKYTV DKRFGMDFKE IELIGSGGFG QVFKAKHRID GKTYVIKRVK YNNEKAEREV KALAKLDHVN IVHYNGCWDG FDYDPETSDD SLESSDYDPE NSKNSSRSKT KCLFIQMEFC DKGT (1-373 aa encoded by BC093676)

Target Information

EIF2AK2 (PKR) is one of 4 kinases that specifically phosphorylate Ser51 of translation initiation factor eIF2-alpha in response to various environmental stresses. EIF2AK2 plays a key role in antiviral defense mediated through its direct activation by double-stranded RNA produced by viral infection. PKR plays a key role in IFN induced-innate antiviral response, virus-induced apoptosis, cell growth & differentiation. PKR induces apoptosis by up-regulating Fas expression and mediates FADD/Caspase-8 death signaling pathway. Upon viral infection, it functions as a dual protein, sequentially activating both cell survival & cell death pathways using kinase independent and dependent strategies. PRKR regulates multiple pathways which include NF-kappaB activation, p53, p38, & PDGF signaling pathway. PKR has been implicated in tumor suppression & malignancy. To evade the antiviral effects of PKR, viruses have evolved multiple mechanisms, such as the inhibition of PKR by the non-structural protein (NS1) of the influenza virus. More recently, PKR has been implicated in several neurodegenerative diseases including Alzheimer, Huntington, and amyotrophic lateral sclerosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Wed Feb 01 00:00:00 UTC 2023

Cryptosporidium uses CSpV1 to activate host type I interferon and attenuate antiparasitic defenses.

Nature communications

Deng S,He W,Gong AY,Li M,Wang Y,Xia Z,Zhang XT,Huang Pacheco AS,Naqib A,Jenkins M,Swanson PC,Drescher KM,Strauss-Soukup JK,Belshan M,Chen XM

Thu Mar 16 00:00:00 UTC 2023

Dynamic mapping of proteome trafficking within and between living cells by TransitID.

bioRxiv : the preprint server for biology

Xu WQ,Cheah JS,Xu C,Messing J,Freibaum BD,Boeynaems S,Taylor JP,Udeshi ND,Carr SA,Ting AY

Wed Feb 08 00:00:00 UTC 2023

Microglial NLRP3 inflammasome activation mediates diabetes-induced depression-like behavior via triggering neuroinflammation.

Progress in neuro-psychopharmacology & biological psychiatry

Su WJ,Li JM,Zhang T,Cao ZY,Hu T,Zhong SY,Xu ZY,Gong H,Jiang CL

Wed Aug 30 00:00:00 UTC 2023

The Amino Acid at Position 95 in the Matrix Protein of Rabies Virus Is Involved in Antiviral Stress Granule Formation in Infected Cells.

Journal of virology

Kojima I,Onomoto K,Zuo W,Ozawa M,Okuya K,Naitou K,Izumi F,Okajima M,Fujiwara T,Ito N,Yoneyama M,Yamada K,Nishizono A,Sugiyama M,Fujita T,Masatani T

Wed Sep 28 00:00:00 UTC 2022

Salvianolic acid A regulates pyroptosis of endothelial cells via directly targeting PKM2 and ameliorates diabetic atherosclerosis.

Frontiers in pharmacology

Zhu J,Chen H,Le Y,Guo J,Liu Z,Dou X,Lu D

Sat Nov 26 00:00:00 UTC 2022

Cardiovirus leader proteins retarget RSK kinases toward alternative substrates to perturb nucleocytoplasmic traffic.

PLoS pathogens

Lizcano-Perret B,Lardinois C,Wavreil F,Hauchamps P,Herinckx G,Sorgeloos F,Vertommen D,Gatto L,Michiels T

Thu Dec 01 00:00:00 UTC 2022

Temporal control of the integrated stress response by a stochastic molecular switch.

Science advances

Klein P,Kallenberger SM,Roth H,Roth K,Ly-Hartig TBN,Magg V,Aleš J,Talemi SR,Qiang Y,Wolf S,Oleksiuk O,Kurilov R,Di Ventura B,Bartenschlager R,Eils R,Rohr K,Hamprecht FA,Höfer T,Fackler OT,Stoecklin G,Ruggieri A

Fri Mar 25 00:00:00 UTC 2022

USP19 (ubiquitin specific peptidase 19) promotes TBK1 (TANK-binding kinase 1) degradation via chaperone-mediated autophagy.

Autophagy

Zhao X,Di Q,Yu J,Quan J,Xiao Y,Zhu H,Li H,Ling J,Chen W

Fri Apr 01 00:00:00 UTC 2022

LincRNA-EPS impairs host antiviral immunity by antagonizing viral RNA-PKR interaction.

EMBO reports

Zhu J,Chen S,Sun LQ,Liu S,Bai X,Li D,Zhang F,Qiao Z,Li L,Yao H,Xia Y,Xu P,Jiang X,Chen Z,Yan Y,Ma F

Wed May 04 00:00:00 UTC 2022

What goes around comes around: Artificial circular RNAs bypass cellular antiviral responses.

Molecular therapy. Nucleic acids

Breuer J,Barth P,Noe Y,Shalamova L,Goesmann A,Weber F,Rossbach O

Tue Jun 14 00:00:00 UTC 2022

Attenuation of Activated eIF2α Signaling by ISRIB Treatment After Spinal Cord Injury Improves Locomotor Function.

Journal of molecular neuroscience : MN

Chang L,Liu X,Chen J,Liu H,Wang G,Wang G,Liao X,Shen X

Tue Mar 01 00:00:00 UTC 2022

Attenuation of Activated eIF2α Signaling by ISRIB Treatment After Spinal Cord Injury Improves Locomotor Function.

Journal of molecular neuroscience : MN

Chang L,Liu X,Chen J,Liu H,Wang G,Wang G,Liao X,Shen X

Tue Mar 01 00:00:00 UTC 2022

Viral evasion of PKR restriction by reprogramming cellular stress granules.

Proceedings of the National Academy of Sciences of the United States of America

Gao P,Liu Y,Wang H,Chai Y,Weng W,Zhang Y,Zhou L,Ge X,Guo X,Han J,Yang H

Tue Jul 19 00:00:00 UTC 2022

Bioinformatics analysis combined with experiments to verify potential autophagy genes in wound healing.

Annals of translational medicine

Wu YF,Fang DL,Wei J

Wed Jun 01 00:00:00 UTC 2022

Maternal immune activation in mice disrupts proteostasis in the fetal brain.

Nature neuroscience

Kalish BT,Kim E,Finander B,Duffy EE,Kim H,Gilman CK,Yim YS,Tong L,Kaufman RJ,Griffith EC,Choi GB,Greenberg ME,Huh JR

Mon Feb 01 00:00:00 UTC 2021

PKR activity modulation by phosphomimetic mutations of serine residues located three aminoacids upstream of double-stranded RNA binding motifs.

Scientific reports

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Wed Apr 28 00:00:00 UTC 2021

PKR activity modulation by phosphomimetic mutations of serine residues located three aminoacids upstream of double-stranded RNA binding motifs.

Scientific reports

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Wed Apr 28 00:00:00 UTC 2021

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Wed Apr 01 00:00:00 UTC 2020

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Journal of virology

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Tue Oct 01 00:00:00 UTC 2019

The Leader Protein of Theiler's Virus Prevents the Activation of PKR.

Journal of virology

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Tue Oct 01 00:00:00 UTC 2019

Oxytocin Suppresses Inflammatory Responses Induced by Lipopolysaccharide through Inhibition of the eIF-2-ATF4 Pathway in Mouse Microglia.

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PKR knockout in the 5xFAD model of Alzheimer's disease reveals beneficial effects on spatial memory and brain lesions.

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Journal of virology

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Wed Aug 01 00:00:00 UTC 2018

Defining the Role of Stress Granules in Innate Immune Suppression by the Herpes Simplex Virus 1 Endoribonuclease VHS.

Journal of virology

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Thu Feb 19 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: double stranded RNA activated protein kinase; dsRNA-activated kinase; EC 2.7.11.1; eIF-2 alpha; eIF-2A protein kinase 2; eIF2a Kinase; eukaryotic translation initiation factor 2 alpha kinase 2; Eukaryotic translation initiation factor 2-alpha kinase 2; IFN- type I-induced and dsRNA-activated kinase; IFN-induced and double-stranded RNA-activated kinase; Interferon-induced, double-stranded RNA-activated protein kinase; interferon-inducible elF2alpha kinase; Interferon-inducible RNA-dependent protein kinase; MGC126524; OTTHUMP00000201320; P1/eIF-2A protein kinase; p68 kinase; PKR; protein kinase R; Protein kinase RNA-activated; protein kinase, interferon inducible double stranded RNA dependent; Protein kinase, interferon-inducible double stranded RNA dependent; protein phosphatase 1, regulatory subunit 83; Serine/threonine-protein kinase TIK; T-cell viral integration site; Tyrosine-protein kinase EIF2AK2

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Gene Aliases: 2310047A08Rik; 4732414G15Rik; AI467567; AI747578; EIF2AK1; EIF2AK2; PKR; PPP1R83; PRKR; Tik

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UniProt ID: (Human) P19525, (Mouse) Q03963, (Rat) Q63184

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Entrez Gene ID: (Human) 5610, (Mouse) 19106, (Rat) 54287

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Function(s)

double-stranded RNA binding protein kinase activity protein serine/threonine kinase activity eukaryotic translation initiation factor 2alpha kinase activity non-membrane spanning protein tyrosine kinase activity protein binding ATP binding protein phosphatase type 2A regulator activity poly(A) RNA binding nucleotide binding RNA binding protein tyrosine kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups

Process(es)

activation of MAPKK activity positive regulation of cytokine production immune system process transcription, DNA-templated regulation of transcription, DNA-templated translation protein phosphorylation response to virus response to toxic substance phosphorylation negative regulation of translation endoplasmic reticulum unfolded protein response response to lipopolysaccharide positive regulation of chemokine production positive regulation of stress-activated MAPK cascade response to vitamin E negative regulation of osteoblast proliferation response to interferon-alpha positive regulation of apoptotic process negative regulation of apoptotic process negative regulation of viral genome replication innate immune response protein autophosphorylation positive regulation of NF-kappaB transcription factor activity defense response to virus regulation of NLRP3 inflammasome complex assembly positive regulation of NIK/NF-kappaB signaling regulation of hematopoietic progenitor cell differentiation regulation of hematopoietic stem cell proliferation regulation of hematopoietic stem cell differentiation response to mechanical stimulus response to organic substance peptidyl-tyrosine phosphorylation response to exogenous dsRNA negative regulation of cell proliferation regulation of translational initiation by eIF2 alpha phosphorylation evasion or tolerance by virus of host immune response regulation of protein phosphatase type 2A activity cellular response to amino acid starvation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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EIF2AK2/PKR Polyclonal Antibody

EIF2AK2/PKR Antibody (18244-1-AP) in ICC/IF

Product Details

18244-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

RNA Immunoprecipitation (RIP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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