Proteintech

EIF2B1 Polyclonal Antibody

EIF2B1 Antibody in Immunocytochemistry (ICC/IF)

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EIF2B1 Antibody (18010-1-AP) in ICC/IF

Immunofluorescent analysis of Hela cells, using EIF2B1 antibody 18010-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

EIF2B1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

EIF2B1 Antibody in Immunoprecipitation (IP)

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Product Details

18010-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

EIF2B1 Fusion Protein Ag12509 (1-305 aa encoded by BC103763)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.13 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MDDKELIEY FKSQMKEDPD MASAVAAIRT LLEFLKRDKG ETIQGLRANL TSAIETLCGV DSSVAVSSGG ELFLRFISLA SLEYSDYSKC KKIMIERGEL FLRRISLSRN KIADLCHTFI KDGATILTHA YSRVVLRVLE AAVAAKKRFS VYVTESQPDL SGKKMAKALC HLNVPVTVVL DAAVGYIMEK ADLVIVGAEG VVENGGIINK IGTNQMAVCA KAQNKPFYVV AESFKFVRLF PLNQQDVPDK FKYKADTLKV AQTGQDLKEE HPWVDYTAPS LITLLFTDLG VLTPSAVSDE LIKLYL (1-305 aa encoded by B C103763)

Target Information

This gene encodes one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in this gene and the genes encoding other EIF2B subunits have been associated with leukoencephalopathy with vanishing white matter.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

eIF2B conformation and assembly state regulate the integrated stress response.

eLife

Schoof M,Boone M,Wang L,Lawrence R,Frost A,Walter P

Wed Mar 10 00:00:00 UTC 2021

Sugar phosphate activation of the stress sensor eIF2B.

Nature communications

Hao Q,Heo JM,Nocek BP,Hicks KG,Stoll VS,Remarcik C,Hackett S,LeBon L,Jain R,Eaton D,Rutter J,Wong YL,Sidrauski C

Tue Jun 08 00:00:00 UTC 2021

eIF2B as a Target for Viral Evasion of PKR-Mediated Translation Inhibition.

mBio

Wuerth JD,Habjan M,Kainulainen M,Berisha B,Bertheloot D,Superti-Furga G,Pichlmair A,Weber F

Tue Jul 14 00:00:00 UTC 2020

Inhibition of the integrated stress response by viral proteins that block p-eIF2-eIF2B association.

Nature microbiology

Rabouw HH,Visser LJ,Passchier TC,Langereis MA,Liu F,Giansanti P,van Vliet ALW,Dekker JG,van der Grein SG,Saucedo JG,Anand AA,Trellet ME,Bonvin AMJJ,Walter P,Heck AJR,de Groot RJ,van Kuppeveld FJM

Sun Nov 01 00:00:00 UTC 2020

Inhibition of the integrated stress response by viral proteins that block p-eIF2-eIF2B association.

Nature microbiology

Rabouw HH,Visser LJ,Passchier TC,Langereis MA,Liu F,Giansanti P,van Vliet ALW,Dekker JG,van der Grein SG,Saucedo JG,Anand AA,Trellet ME,Bonvin AMJJ,Walter P,Heck AJR,de Groot RJ,van Kuppeveld FJM

Sun Nov 01 00:00:00 UTC 2020

Cellular eIF2B subunit localization: implications for the integrated stress response and its control by small molecule drugs.

Molecular biology of the cell

Hodgson RE,Varanda BA,Ashe MP,Allen KE,Campbell SG

Mon Apr 01 00:00:00 UTC 2019

The small molecule ISRIB rescues the stability and activity of Vanishing White Matter Disease eIF2B mutant complexes.

eLife

Wong YL,LeBon L,Edalji R,Lim HB,Sun C,Sidrauski C

Wed Feb 28 00:00:00 UTC 2018

Stoichiometry of the eIF2B complex is maintained by mutual stabilization of subunits.

The Biochemical journal

Wortham NC,Stewart JD,Harris S,Coldwell MJ,Proud CG

Tue Mar 01 00:00:00 UTC 2016

Stoichiometry of the eIF2B complex is maintained by mutual stabilization of subunits.

The Biochemical journal

Wortham NC,Stewart JD,Harris S,Coldwell MJ,Proud CG

Tue Mar 01 00:00:00 UTC 2016

Pharmacological dimerization and activation of the exchange factor eIF2B antagonizes the integrated stress response.

eLife

Sidrauski C,Tsai JC,Kampmann M,Hearn BR,Vedantham P,Jaishankar P,Sokabe M,Mendez AS,Newton BW,Tang EL,Verschueren E,Johnson JR,Krogan NJ,Fraser CS,Weissman JS,Renslo AR,Walter P

Wed Apr 15 00:00:00 UTC 2015

Pharmacological dimerization and activation of the exchange factor eIF2B antagonizes the integrated stress response.

eLife

Sidrauski C,Tsai JC,Kampmann M,Hearn BR,Vedantham P,Jaishankar P,Sokabe M,Mendez AS,Newton BW,Tang EL,Verschueren E,Johnson JR,Krogan NJ,Fraser CS,Weissman JS,Renslo AR,Walter P

Wed Apr 15 00:00:00 UTC 2015

Analysis of the subunit organization of the eIF2B complex reveals new insights into its structure and regulation.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Wortham NC,Martinez M,Gordiyenko Y,Robinson CV,Proud CG

Thu May 01 00:00:00 UTC 2014

Analysis of the subunit organization of the eIF2B complex reveals new insights into its structure and regulation.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Wortham NC,Martinez M,Gordiyenko Y,Robinson CV,Proud CG

Thu May 01 00:00:00 UTC 2014

Analysis of the subunit organization of the eIF2B complex reveals new insights into its structure and regulation.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Wortham NC,Martinez M,Gordiyenko Y,Robinson CV,Proud CG

Thu May 01 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: eIF-2B GDP-GTP exchange factor subunit alpha; eIF2B GDP-GTP exchange factor subunit alpha; eukaryotic translation initiation factor 2B, subunit 1 (alpha, 26kDa); eukaryotic translation initiation factor 2B, subunit 1 alpha, 26kDa; translation initiation factor eIF-2B subunit alpha; Translation initiation factor eIF2B subunit alpha

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Gene Aliases: 26kDa; D5Ertd406e; EIF2B; EIF2B1; EIF2BA

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UniProt ID: (Human) Q14232, (Mouse) Q99LC8

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Entrez Gene ID: (Human) 1967, (Mouse) 209354

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Function(s)

translation initiation factor activity guanyl-nucleotide exchange factor activity protein binding S-methyl-5-thioribose-1-phosphate isomerase activity GTP binding GDP binding enzyme regulator activity

Process(es)

translational initiation regulation of translational initiation response to heat response to glucose oligodendrocyte development L-methionine biosynthetic process from methylthioadenosine response to peptide hormone positive regulation of GTPase activity cellular response to stimulus translation regulation of translation negative regulation of translational initiation in response to stress cellular metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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EIF2B1 Polyclonal Antibody

EIF2B1 Antibody (18010-1-AP) in ICC/IF

Product Details

18010-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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