Proteintech

EIF2B2 Polyclonal Antibody

EIF2B2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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EIF2B2 Antibody (11034-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded Mouse brain tissue slide using (Product # 11034-1-AP) EIF2B2 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

EIF2B2 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

11034-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Immunoprecipitation (IP)

4 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

EIF2B2 Fusion Protein Ag1502 (1-351 aa encoded by BC011750)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.24 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MPGSAAKGS ELSERIESFV ETLKRGGGPR SSEEMARETL GLLRQIITDH RWSNAGELME LIRREGRRMT AAQPSETTVG NMVRRVLKII REEYGRLHGR SDESDQQESL HKLLTSGGLN EDFSFHYAQL QSNIIEAINE LLVELEGTME NIAAQALEHI HSNEVIMTIG FSRTVEAFLK EAARKRKFHV IVAECAPFCQ GHEMAVNLSK AGIETTVMTD AAIFAVMSRV NKVIIGTKTI LANGALRAVT GTHTLALAAK HHSTPLIVCA PMFKLSPQFP NEEDSFHKFV APEEVLPFTE GDILEKVSVH CPVFDYVPPE LITLFISNIG GNAPSYIYRL MSELYHPDDH VL (1-351 aa encoded by BC011750)

Target Information

eIF2b beta is the beta subunit of eukaryotic initiation factor-2B (EIF2B). EIF2B is involved in protein synthesis and exchanges GDP and GTP for its activation and deactivation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

DIAPH3 condensates formed by liquid-liquid phase separation act as a regulatory hub for stress-induced actin cytoskeleton remodeling.

Cell reports

Zhang K,Huang M,Li A,Wen J,Yan L,Li Y,Guo L,Senthil KS,Zhou Y,Chen G,Liu Y,Zhang X,Yao X,Qin D,Su H

Tue Jan 31 00:00:00 UTC 2023

A point mutation in the nucleotide exchange factor eIF2B constitutively activates the integrated stress response by allosteric modulation.

eLife

Boone M,Wang L,Lawrence RE,Frost A,Walter P,Schoof M

Wed Apr 13 00:00:00 UTC 2022

eIF2B conformation and assembly state regulate the integrated stress response.

eLife

Schoof M,Boone M,Wang L,Lawrence R,Frost A,Walter P

Wed Mar 10 00:00:00 UTC 2021

Cellular eIF2B subunit localization: implications for the integrated stress response and its control by small molecule drugs.

Molecular biology of the cell

Hodgson RE,Varanda BA,Ashe MP,Allen KE,Campbell SG

Mon Apr 01 00:00:00 UTC 2019

Proteomic analysis of seminal extracellular vesicle proteins involved in asthenozoospermia by iTRAQ.

Molecular reproduction and development

Lin Y,Liang A,He Y,Li Z,Li Z,Wang G,Sun F

Sun Sep 01 00:00:00 UTC 2019

EIF2B2 mutations in vanishing white matter disease hypersuppress translation and delay recovery during the integrated stress response.

RNA (New York, N.Y.)

Moon SL,Parker R

Fri Jun 01 00:00:00 UTC 2018

EIF2B2 mutations in vanishing white matter disease hypersuppress translation and delay recovery during the integrated stress response.

RNA (New York, N.Y.)

Moon SL,Parker R

Fri Jun 01 00:00:00 UTC 2018

The small molecule ISRIB rescues the stability and activity of Vanishing White Matter Disease eIF2B mutant complexes.

eLife

Wong YL,LeBon L,Edalji R,Lim HB,Sun C,Sidrauski C

Wed Feb 28 00:00:00 UTC 2018

Stoichiometry of the eIF2B complex is maintained by mutual stabilization of subunits.

The Biochemical journal

Wortham NC,Stewart JD,Harris S,Coldwell MJ,Proud CG

Tue Mar 01 00:00:00 UTC 2016

Stoichiometry of the eIF2B complex is maintained by mutual stabilization of subunits.

The Biochemical journal

Wortham NC,Stewart JD,Harris S,Coldwell MJ,Proud CG

Tue Mar 01 00:00:00 UTC 2016

Pharmacological dimerization and activation of the exchange factor eIF2B antagonizes the integrated stress response.

eLife

Sidrauski C,Tsai JC,Kampmann M,Hearn BR,Vedantham P,Jaishankar P,Sokabe M,Mendez AS,Newton BW,Tang EL,Verschueren E,Johnson JR,Krogan NJ,Fraser CS,Weissman JS,Renslo AR,Walter P

Wed Apr 15 00:00:00 UTC 2015

Pharmacological dimerization and activation of the exchange factor eIF2B antagonizes the integrated stress response.

eLife

Sidrauski C,Tsai JC,Kampmann M,Hearn BR,Vedantham P,Jaishankar P,Sokabe M,Mendez AS,Newton BW,Tang EL,Verschueren E,Johnson JR,Krogan NJ,Fraser CS,Weissman JS,Renslo AR,Walter P

Wed Apr 15 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: eIF-2B GDP-GTP exchange factor subunit beta; eIF2B GDP-GTP exchange factor subunit beta; eukaryotic translation initiation factor 2B, subunit 2 (beta, 39kD); eukaryotic translation initiation factor 2B, subunit 2 beta, 39kDa; S20I15; S20III15; translation initiation factor eIF-2B subunit beta; Translation initiation factor eIF2B subunit beta

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Gene Aliases: AA409345; C85417; EIF-2Bbeta; EIF2B; EIF2B2; EIF2BB

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UniProt ID: (Human) P49770, (Mouse) Q99LD9

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Entrez Gene ID: (Human) 8892, (Mouse) 217715, (Rat) 84005

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Function(s)

translation initiation factor activity guanyl-nucleotide exchange factor activity protein binding ATP binding GTP binding S-methyl-5-thioribose-1-phosphate isomerase activity

Process(es)

ovarian follicle development translation translational initiation regulation of translational initiation central nervous system development response to heat response to glucose oligodendrocyte development myelination response to peptide hormone positive regulation of GTPase activity cellular metabolic process cellular response to stimulus L-methionine biosynthetic process from methylthioadenosine

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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EIF2B2 Polyclonal Antibody

EIF2B2 Antibody (11034-1-AP) in IHC (P)

Product Details

11034-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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