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Invitrogen
EIF2B4 Polyclonal Antibody
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EIF2B4 Antibody (PA5-30292) in ICC/IF
Immunofluorescence analysis of Translation initiation factor eIF-2B subunit delta was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with eIF2b delta Polyclonal Antibody (Product # PA5-30292) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombi... View More
Product Details
PA5-30292
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human EIF2B delta. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.61 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2547766
Product Specific Information
Recommended positive controls: 293T, A431, Raji.
Predicted reactivity: Chimpanzee (97%), Bovine (86%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Eukaryotic initiation factor 2B (EIF2B), which is necessary for protein synthesis, is a GTP exchange factor composed of five different subunits. The protein encoded by this gene is the fourth, or delta, subunit. Defects in this gene are a cause of leukoencephalopathy with vanishing white matter (VWM) and ovarioleukodystrophy. Multiple transcript variants encoding different isoforms have been found for this gene.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp586J0119; eIF-2B GDP-GTP exchange factor subunit delta; eIF2B GDP-GTP exchange factor subunit delta; eukaryotic translation initiation factor 2B subunit 4 delta; eukaryotic translation initiation factor 2B, subunit 4 delta, 67kDa; translation initiation factor eIF-2b delta subunit; translation initiation factor eIF-2B subunit delta; Translation initiation factor eIF2B subunit delta
Gene Aliases: EIF-2B; EIF2B; EIF2B4; EIF2BD; EIF2Bdelta; Jgr1a
UniProt ID: (Human) Q9UI10, (Rat) Q63186, (Mouse) Q61749
Entrez Gene ID: (Human) 8890, (Rat) 117019, (Mouse) 13667
ovarian follicle development
translational initiation
regulation of translation
response to heat
response to glucose
oligodendrocyte development
L-methionine biosynthetic process from methylthioadenosine
negative regulation of translational initiation in response to stress
myelination
response to peptide hormone
positive regulation of GTPase activity
cellular metabolic process
translation
negative regulation of translational initiation
cellular response to stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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