Invitrogen

EIF4A1/EIF4A2/EIF4A3 Polyclonal Antibody

View all (2) EIF4A1/EIF4A2/EIF4A3 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-116950

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

Immunocytochemistry (ICC/IF)

1:50-1:200
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human eIF4A (NP_0014071)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.638 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2901580

Product Specific Information

Positive Samples: 293T, HeLa, HepG2, MCF7, C2C12, Mouse brain, Rat brain

Immunogen sequence: MSASQDSRSR DNGPDGMEPE GVIESNWNEI VDSFDDMNLS ESLLRGIYAY GFEKPSAIQQ RAILPCIKGY DVIAQAQSGT GKTATFAISI LQQIELDLKA

Target Information

The eIF4A proteins are involved in many aspects of RNA metabolism due to their RNA-binding capacity and ATPase activity. eIF4A1 and eIF4A2 are cytoplasmic, whereas eIF4A3 is nuclear-localized. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ATP-dependent RNA helicase DDX48; ATP-dependent RNA helicase eIF4A-1; ATP-dependent RNA helicase eIF4A-2; ATP-dependent RNA helicase eIF4A-3; DEAD (Asp-Glu-Ala-Asp) box polypeptide 48; DEAD box protein 48; eIF-4A-I; eIF-4A-II; eIF-4A-III; eIF4A-I; eIF4A-II; eIF4A-III; Eukaryotic initiation factor 4A-I; Eukaryotic initiation factor 4A-II; Eukaryotic initiation factor 4A-III; Eukaryotic initiation factor 4A-like NUK-34; eukaryotic initiation factor 4AI; Eukaryotic translation initiation factor 4A isoform 3; eukaryotic translation initiation factor 4A, isoform 1; eukaryotic translation initiation factor 4A, isoform 2; eukaryotic translation initiation factor 4A, isoform 3; hNMP 265; initiation factor eIF-4A long form; NMP 265; Nuclear matrix protein 265; protein synthesis initiation factor 4AII

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Gene Aliases: 2400003O03Rik; 4833432N07Rik; BM-010; DDX2A; DDX2B; DDX48; EIF-4A; eIF-4A-I; eIF-4A-II; Eif4; EIF4A; eIF4A-I; eIF4A-II; eIF4A-III; EIF4A1; EIF4A2; EIF4A3; eIF4AIII; EIF4F; KIAA0111; mKIAA0111; MUK34; NMP265; NUK34; RCPS

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UniProt ID: (Human) P60842, (Human) Q14240, (Human) P38919, (Mouse) P60843, (Mouse) P10630, (Rat) Q5RKI1, (Mouse) Q91VC3, (Rat) Q3B8Q2

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Entrez Gene ID: (Human) 1973, (Human) 1974, (Human) 9775, (Mouse) 13681, (Rat) 287436, (Mouse) 13682, (Rat) 303831, (Mouse) 192170, (Rat) 688288

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Function(s)
RNA cap binding double-stranded RNA binding mRNA binding translation initiation factor activity ATP-dependent RNA helicase activity helicase activity protein binding ATP binding translation factor activity, RNA binding poly(A) RNA binding ATPase activity poly(A) binding selenocysteine insertion sequence binding RNA stem-loop binding ribonucleoprotein complex binding nucleotide binding nucleic acid binding RNA binding hydrolase activity molecular_function
Process(es)
nuclear-transcribed mRNA catabolic process, nonsense-mediated decay nuclear-transcribed mRNA poly(A) tail shortening mRNA splicing, via spliceosome rRNA processing termination of RNA polymerase II transcription RNA export from nucleus mRNA export from nucleus associative learning RNA splicing RNA secondary structure unwinding response to organic cyclic compound selenocysteine metabolic process negative regulation of translation mRNA 3'-end processing exploration behavior positive regulation of translation embryonic cranial skeleton morphogenesis cellular response to selenite ion negative regulation of excitatory postsynaptic potential negative regulation of selenocysteine incorporation negative regulation of selenocysteine insertion sequence binding cellular response to brain-derived neurotrophic factor stimulus translational initiation regulation of translational initiation viral process negative regulation of RNA-directed RNA polymerase activity regulation of gene expression organ regeneration translation positive regulation of transcription from RNA polymerase II promoter positive regulation of mRNA splicing, via spliceosome regulation of alternative mRNA splicing, via spliceosome mRNA processing mRNA transport
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