Proteintech

EIF4A3 Polyclonal Antibody

EIF4A3 Antibody in Immunocytochemistry (ICC/IF)

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EIF4A3 Antibody (17504-1-AP) in ICC/IF

Immunofluorescent analysis of MCF-7 cells, using EIF4A3 antibody 17504-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

EIF4A3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

EIF4A3 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

17504-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 21 publications 21 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 7 publications 7 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

RNA Immunoprecipitation (RIP)

View 7 publications 7 publications

Miscellaneous PubMed (MISC)

View 3 publications 3 publications

Not applicable (N/A)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

EIF4A3 Fusion Protein Ag11701 (1-411 aa encoded by BC003662)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.35 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MATTATMAT SGSARKRLLK EEDMTKVEFE TSEEVDVTPT FDTMGLREDL LRGIYAYGFE KPSAIQQRAI KQIIKGRDVI AQSQSGTGKT ATFSISVLQC LDIQVRETQA LILAPTRELA VQIQKGLLAL GDYMNVQCHA CIGGTNVGED IRKLDYGQHV VAGTPGRVFD MIRRRSLRTR AIKMLVLDEA DEMLNKGFKE QIYDVYRYLP PATQVVLISA TLPHEILEMT NKFMTDPIRI LVKRDELTLE GIKQFFVAVE REEWKFDTLC DLYDTLTITQ AVIFCNTKRK VDWLTEKMRE ANFTVSSMHG DMPQKERESI MKEFRSGASR VLISTDVWAR GLDVPQVSLI INYDLPNNRE LYIHRIGRSG RYGRKGVAIN FVKNDDIRIL RDIEQYYSTQ IDEMPMNVAD LI (1-411 aa encoded by BC003662)

Target Information

eIF4A3 is a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. eIF4A3 is a nuclear matrix protein. Its amino acid sequence is highly similar to the amino acid sequences of the translation initiation factors eIF4AI and eIF4AII, two other members of the DEAD box protein family.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

EIF4A3-Induced Upregulation of hsa_circ_0040039 is a Biomarker and Aggravates IL-1β-Stimulated Intervertebral Disc Degeneration.

Applied biochemistry and biotechnology

Li L,Cao Y,Deng H,Jia J,Fan X

Sat Jul 01 00:00:00 UTC 2023

Exosome-transmitted circCABIN1 promotes temozolomide resistance in glioblastoma via sustaining ErbB downstream signaling.

Journal of nanobiotechnology

Liu X,Guo Q,Gao G,Cao Z,Guan Z,Jia B,Wang W,Zhang K,Zhang W,Wang S,Li W,Hao Q,Zhang Y,Li M,Zhang W,Gu J

Wed Feb 08 00:00:00 UTC 2023

Epitranscriptic regulation of HRAS by N⁶-methyladenosine drives tumor progression.

Proceedings of the National Academy of Sciences of the United States of America

Pan Y,Gu Y,Liu T,Zhang Q,Yang F,Duan L,Cheng S,Zhu X,Xi Y,Chang X,Ye Q,Gao S

Tue Apr 04 00:00:00 UTC 2023

EIF4A3-induced Circ_0001187 facilitates AML suppression through promoting ubiquitin-proteasomal degradation of METTL3 and decreasing m6A modification level mediated by miR-499a-5p/RNF113A pathway.

Biomarker research

Yang X,Han F,Hu X,Li G,Wu H,Can C,Wei Y,Liu J,Wang R,Jia W,Ji C,Ma D

Tue Jun 06 00:00:00 UTC 2023

CircKIF4A combines EIF4A3 to stabilize SDC1 expression to activate c-src/FAK and promotes TNBC progression.

Cellular signalling

Luo P,Gong Y,Weng J,Wen F,Luo J,Hu C,Xiao J,Shu J

Tue Aug 01 00:00:00 UTC 2023

The proteome and transcriptome of stress granules and P bodies during human T lymphocyte activation.

Cell reports

Curdy N,Lanvin O,Cerapio JP,Pont F,Tosolini M,Sarot E,Valle C,Saint-Laurent N,Lhuillier E,Laurent C,Fournié JJ,Franchini DM

Tue Mar 28 00:00:00 UTC 2023

Splicing factor SF3B3, a NS5-binding protein, restricts ZIKV infection by targeting GCH1.

Virologica Sinica

Chen T,Yang H,Liu P,Hamiti M,Zhang X,Xu Y,Quan W,Zhang Y,Yu W,Jiao L,Du T,Xi J,Yin B,Zhou W,Lu S,Peng X

Sat Apr 01 00:00:00 UTC 2023

Splicing factor SF3B3, a NS5-binding protein, restricts ZIKV infection by targeting GCH1.

Virologica Sinica

Chen T,Yang H,Liu P,Hamiti M,Zhang X,Xu Y,Quan W,Zhang Y,Yu W,Jiao L,Du T,Xi J,Yin B,Zhou W,Lu S,Peng X

Sat Apr 01 00:00:00 UTC 2023

EIF4A3-mediated circPRKCI expression promotes triple-negative breast cancer progression by regulating WBP2 and PI3K/AKT signaling pathway.

Cell death discovery

Wang X,Song H,Fang L,Wu T

Wed Mar 02 00:00:00 UTC 2022

eIF4A3 Promotes RNA Viruses' Replication by Inhibiting Innate Immune Responses.

Journal of virology

Gao Q,Jiang M,Zhao Y,Li G,Yang C,Ren C,Jin M,Chen H,Zhou H

Wed Nov 23 00:00:00 UTC 2022

CircMYH9 increases KPNA2 mRNA stability to promote hepatocellular carcinoma progression in an EIF4A3-dependent manner.

American journal of cancer research

Xia J,Wu C,Tang Y,Tang J,Zhu D,Zhang F,Xu Z,Sun D,Tan Z,Zhuo H

Fri Oct 14 00:00:00 UTC 2022

An RNAi screen of RNA helicases identifies eIF4A3 as a regulator of embryonic stem cell identity.

Nucleic acids research

Li D,Yang J,Malik V,Huang Y,Huang X,Zhou H,Wang J

Mon Nov 28 00:00:00 UTC 2022

An RNAi screen of RNA helicases identifies eIF4A3 as a regulator of embryonic stem cell identity.

Nucleic acids research

Li D,Yang J,Malik V,Huang Y,Huang X,Zhou H,Wang J

Mon Nov 28 00:00:00 UTC 2022

An RNAi screen of RNA helicases identifies eIF4A3 as a regulator of embryonic stem cell identity.

Nucleic acids research

Li D,Yang J,Malik V,Huang Y,Huang X,Zhou H,Wang J

Mon Nov 28 00:00:00 UTC 2022

Local translation in nuclear condensate amyloid bodies.

Proceedings of the National Academy of Sciences of the United States of America

Theodoridis PR,Bokros M,Marijan D,Balukoff NC,Wang D,Kirk CC,Budine TD,Goldsmith HD,Wang M,Audas TE,Lee S

Tue Feb 16 00:00:00 UTC 2021

hsa_circ_0068631 promotes breast cancer progression through c-Myc by binding to EIF4A3.

Molecular therapy. Nucleic acids

Wang X,Chen M,Fang L

Fri Dec 03 00:00:00 UTC 2021

hsa_circ_0068631 promotes breast cancer progression through c-Myc by binding to EIF4A3.

Molecular therapy. Nucleic acids

Wang X,Chen M,Fang L

Fri Dec 03 00:00:00 UTC 2021

LRRC4 mediates the formation of circular RNA CD44 to inhibitGBM cell proliferation.

Molecular therapy. Nucleic acids

Feng J,Ren X,Fu H,Li D,Chen X,Zu X,Liu Q,Wu M

Fri Dec 03 00:00:00 UTC 2021

LRRC4 mediates the formation of circular RNA CD44 to inhibitGBM cell proliferation.

Molecular therapy. Nucleic acids

Feng J,Ren X,Fu H,Li D,Chen X,Zu X,Liu Q,Wu M

Fri Dec 03 00:00:00 UTC 2021

LRRC4 mediates the formation of circular RNA CD44 to inhibitGBM cell proliferation.

Molecular therapy. Nucleic acids

Feng J,Ren X,Fu H,Li D,Chen X,Zu X,Liu Q,Wu M

Fri Dec 03 00:00:00 UTC 2021

NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.

Cell metabolism

Park MK,Zhang L,Min KW,Cho JH,Yeh CC,Moon H,Hormaechea-Agulla D,Mun H,Ko S,Lee JW,Jathar S,Smith AS,Yao Y,Giang NT,Vu HH,Yan VC,Bridges MC,Kourtidis A,Muller F,Chang JH,Song SJ,Nakagawa S,Hirose T,Yoon JH,Song MS

Tue Dec 07 00:00:00 UTC 2021

CircSPI1 acts as an oncogene in acute myeloid leukemia through antagonizing SPI1 and interacting with microRNAs.

Cell death & disease

Wang X,Jin P,Zhang Y,Wang K

Fri Mar 19 00:00:00 UTC 2021

eIF4A3 regulates the TFEB-mediated transcriptional response via GSK3B to control autophagy.

Cell death and differentiation

Sakellariou D,Tiberti M,Kleiber TH,Blazquez L,López AR,Abildgaard MH,Lubas M,Bartek J,Papaleo E,Frankel LB

Wed Dec 01 00:00:00 UTC 2021

eIF4A3 regulates the TFEB-mediated transcriptional response via GSK3B to control autophagy.

Cell death and differentiation

Sakellariou D,Tiberti M,Kleiber TH,Blazquez L,López AR,Abildgaard MH,Lubas M,Bartek J,Papaleo E,Frankel LB

Wed Dec 01 00:00:00 UTC 2021

Comprehensive analysis of biological networks and the eukaryotic initiation factor 4A-3 gene as pivotal in hepatocellular carcinoma.

Journal of cellular biochemistry

Lin Y,Liang R,Mao Y,Ye J,Mai R,Gao X,Liu Z,Wainwright T,Li Q,Luo M,Ge L,Li Y,Zou D

Thu Oct 01 00:00:00 UTC 2020

LINC00680 and TTN-AS1 Stabilized by EIF4A3 Promoted Malignant Biological Behaviors of Glioblastoma Cells.

Molecular therapy. Nucleic acids

Tang W,Wang D,Shao L,Liu X,Zheng J,Xue Y,Ruan X,Yang C,Liu L,Ma J,Li Z,Liu Y

Fri Mar 06 00:00:00 UTC 2020

Long noncoding RNA ZFAS1 promoting small nucleolar RNA-mediated 2'-O-methylation via NOP58 recruitment in colorectal cancer.

Molecular cancer

Wu H,Qin W,Lu S,Wang X,Zhang J,Sun T,Hu X,Li Y,Chen Q,Wang Y,Zhao H,Piao H,Zhang R,Wei M

Fri May 22 00:00:00 UTC 2020

Human nuclear RNAi-defective 2 (NRDE2) is an essential RNA splicing factor.

RNA (New York, N.Y.)

Jiao AL,Perales R,Umbreit NT,Haswell JR,Piper ME,Adams BD,Pellman D,Kennedy S,Slack FJ

Fri Mar 01 00:00:00 UTC 2019

Human nuclear RNAi-defective 2 (NRDE2) is an essential RNA splicing factor.

RNA (New York, N.Y.)

Jiao AL,Perales R,Umbreit NT,Haswell JR,Piper ME,Adams BD,Pellman D,Kennedy S,Slack FJ

Fri Mar 01 00:00:00 UTC 2019

LncRNA GABPB1-AS1 and GABPB1 regulate oxidative stress during erastin-induced ferroptosis in HepG2 hepatocellular carcinoma cells.

Scientific reports

Qi W,Li Z,Xia L,Dai J,Zhang Q,Wu C,Xu S

Thu Nov 07 00:00:00 UTC 2019

LncRNA CASC9 interacts with CPSF3 to regulate TGF-β signaling in colorectal cancer.

Journal of experimental & clinical cancer research : CR

Luo K,Geng J,Zhang Q,Xu Y,Zhou X,Huang Z,Shi KQ,Pan C,Wu J

Tue Jun 11 00:00:00 UTC 2019

YTHDF3 facilitates translation and decay of N⁶-methyladenosine-modified RNA.

Cell research

Shi H,Wang X,Lu Z,Zhao BS,Ma H,Hsu PJ,Liu C,He C

Wed Mar 01 00:00:00 UTC 2017

YTHDF3 facilitates translation and decay of N⁶-methyladenosine-modified RNA.

Cell research

Shi H,Wang X,Lu Z,Zhao BS,Ma H,Hsu PJ,Liu C,He C

Wed Mar 01 00:00:00 UTC 2017

mRNAs containing NMD-competent premature termination codons are stabilized and translated under UPF1 depletion.

Scientific reports

Kim WK,Yun S,Kwon Y,You KT,Shin N,Kim J,Kim H

Mon Nov 20 00:00:00 UTC 2017

mRNAs containing NMD-competent premature termination codons are stabilized and translated under UPF1 depletion.

Scientific reports

Kim WK,Yun S,Kwon Y,You KT,Shin N,Kim J,Kim H

Mon Nov 20 00:00:00 UTC 2017

Y14 governs p53 expression and modulates DNA damage sensitivity.

Scientific reports

Lu CC,Lee CC,Tseng CT,Tarn WY

Fri Mar 31 00:00:00 UTC 2017

Y14 governs p53 expression and modulates DNA damage sensitivity.

Scientific reports

Lu CC,Lee CC,Tseng CT,Tarn WY

Fri Mar 31 00:00:00 UTC 2017

The RNA-binding profile of Acinus, a peripheral component of the exon junction complex, reveals its role in splicing regulation.

RNA (New York, N.Y.)

Rodor J,Pan Q,Blencowe BJ,Eyras E,Cáceres JF

Thu Sep 01 00:00:00 UTC 2016

The RNA-binding profile of Acinus, a peripheral component of the exon junction complex, reveals its role in splicing regulation.

RNA (New York, N.Y.)

Rodor J,Pan Q,Blencowe BJ,Eyras E,Cáceres JF

Thu Sep 01 00:00:00 UTC 2016

A Point Mutation in the Exon Junction Complex Factor Y14 Disrupts Its Function in mRNA Cap Binding and Translation Enhancement.

The Journal of biological chemistry

Chuang TW,Lee KM,Lou YC,Lu CC,Tarn WY

Fri Apr 15 00:00:00 UTC 2016

A Point Mutation in the Exon Junction Complex Factor Y14 Disrupts Its Function in mRNA Cap Binding and Translation Enhancement.

The Journal of biological chemistry

Chuang TW,Lee KM,Lou YC,Lu CC,Tarn WY

Fri Apr 15 00:00:00 UTC 2016

TDP-43 aggregation mirrors TDP-43 knockdown, affecting the expression levels of a common set of proteins.

Scientific reports

Prpar Mihevc S,Baralle M,Buratti E,Rogelj B

Mon Sep 26 00:00:00 UTC 2016

TDP-43 aggregation mirrors TDP-43 knockdown, affecting the expression levels of a common set of proteins.

Scientific reports

Prpar Mihevc S,Baralle M,Buratti E,Rogelj B

Mon Sep 26 00:00:00 UTC 2016

TDP-43 aggregation mirrors TDP-43 knockdown, affecting the expression levels of a common set of proteins.

Scientific reports

Prpar Mihevc S,Baralle M,Buratti E,Rogelj B

Mon Sep 26 00:00:00 UTC 2016

TDP-43 aggregation mirrors TDP-43 knockdown, affecting the expression levels of a common set of proteins.

Scientific reports

Prpar Mihevc S,Baralle M,Buratti E,Rogelj B

Mon Sep 26 00:00:00 UTC 2016

A proteomic approach to discover and compare interacting partners of papillomavirus E2 proteins from diverse phylogenetic groups.

Proteomics

Jang MK,Anderson DE,van Doorslaer K,McBride AA

Mon Jun 01 00:00:00 UTC 2015

A proteomic approach to discover and compare interacting partners of papillomavirus E2 proteins from diverse phylogenetic groups.

Proteomics

Jang MK,Anderson DE,van Doorslaer K,McBride AA

Mon Jun 01 00:00:00 UTC 2015

Structures of SMG1-UPFs complexes: SMG1 contributes to regulate UPF2-dependent activation of UPF1 in NMD.

Structure (London, England : 1993)

Melero R,Uchiyama A,Castaño R,Kataoka N,Kurosawa H,Ohno S,Yamashita A,Llorca O

Tue Aug 05 00:00:00 UTC 2014

Structures of SMG1-UPFs complexes: SMG1 contributes to regulate UPF2-dependent activation of UPF1 in NMD.

Structure (London, England : 1993)

Melero R,Uchiyama A,Castaño R,Kataoka N,Kurosawa H,Ohno S,Yamashita A,Llorca O

Tue Aug 05 00:00:00 UTC 2014

An atlas of chromatoid body components.

RNA (New York, N.Y.)

Meikar O,Vagin VV,Chalmel F,Sõstar K,Lardenois A,Hammell M,Jin Y,Da Ros M,Wasik KA,Toppari J,Hannon GJ,Kotaja N

Tue Apr 01 00:00:00 UTC 2014

An atlas of chromatoid body components.

RNA (New York, N.Y.)

Meikar O,Vagin VV,Chalmel F,Sõstar K,Lardenois A,Hammell M,Jin Y,Da Ros M,Wasik KA,Toppari J,Hannon GJ,Kotaja N

Tue Apr 01 00:00:00 UTC 2014

A fine balance: Regulation of hippocampal Arc/Arg3.1 transcription, translation and degradation in a rat model of normal cognitive aging.

Neurobiology of learning and memory

Fletcher BR,Hill GS,Long JM,Gallagher M,Shapiro ML,Rapp PR

Sat Nov 01 00:00:00 UTC 2014

A fine balance: Regulation of hippocampal Arc/Arg3.1 transcription, translation and degradation in a rat model of normal cognitive aging.

Neurobiology of learning and memory

Fletcher BR,Hill GS,Long JM,Gallagher M,Shapiro ML,Rapp PR

Sat Nov 01 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: ATP-dependent RNA helicase DDX48; ATP-dependent RNA helicase eIF4A-3; DEAD (Asp-Glu-Ala-Asp) box polypeptide 48; DEAD box protein 48; eIF-4A-III; eIF4A-III; Eukaryotic initiation factor 4A-III; Eukaryotic initiation factor 4A-like NUK-34; Eukaryotic translation initiation factor 4A isoform 3; eukaryotic translation initiation factor 4A, isoform 3; hNMP 265; NMP 265; Nuclear matrix protein 265

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Gene Aliases: 2400003O03Rik; DDX48; eIF4A-III; EIF4A3; eIF4AIII; KIAA0111; mKIAA0111; MUK34; NMP265; NUK34; RCPS

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UniProt ID: (Human) P38919, (Mouse) Q91VC3, (Rat) Q3B8Q2

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Entrez Gene ID: (Human) 9775, (Mouse) 192170, (Rat) 688288

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Function(s)

mRNA binding ATP-dependent RNA helicase activity protein binding ATP binding poly(A) binding selenocysteine insertion sequence binding RNA stem-loop binding ribonucleoprotein complex binding poly(A) RNA binding

Process(es)

nuclear-transcribed mRNA catabolic process, nonsense-mediated decay nuclear-transcribed mRNA poly(A) tail shortening mRNA splicing, via spliceosome rRNA processing termination of RNA polymerase II transcription RNA export from nucleus mRNA export from nucleus associative learning RNA splicing RNA secondary structure unwinding response to organic cyclic compound selenocysteine metabolic process negative regulation of translation mRNA 3'-end processing exploration behavior positive regulation of translation embryonic cranial skeleton morphogenesis cellular response to selenite ion negative regulation of excitatory postsynaptic potential negative regulation of selenocysteine incorporation negative regulation of selenocysteine insertion sequence binding cellular response to brain-derived neurotrophic factor stimulus positive regulation of transcription from RNA polymerase II promoter positive regulation of mRNA splicing, via spliceosome regulation of alternative mRNA splicing, via spliceosome mRNA processing mRNA transport

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EIF4A3 Polyclonal Antibody

EIF4A3 Antibody (17504-1-AP) in ICC/IF

Product Details

17504-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

RNA Immunoprecipitation (RIP)

Miscellaneous PubMed (MISC)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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