Invitrogen

ELF3 Polyclonal Antibody

FIGURE: 1 / 2

ELF3 Antibody (PA5-89261) in WB

Western blot analysis of ELF3 in various lysates. Samples were incubated with ELF3 Polyclonal antibody (Product # PA5-89261) using a dilution of 1:400, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

ELF3 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

PA5-89261

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:500

View 2 publications 2 publications

ELISA (ELISA)

1 µg/mL

Immunoprecipitation (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ESE1/ESE1/ELF3 (NP_0044243)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.44 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2805451

Product Specific Information

Immunogen sequence: MAATCEISNI FSNYFSAMYS SEDSTLASVP PAATFGADDL VLTLSNPQMS LEGTEKASWL GEQPQFWSKT QVLDWISYQV EKNKYDASAI DFSRCDMDGA TLCNCALEEL RLVFGPLGDQ LHAQLRDLTS SSSDELSWII ELLEKDGMAF QEALDPGPFD QGSPFAQELL DDGQQASPYH PGSCGAGAPS PGSSDVSTAG TGASRSSHSS DSGGSDVDLD PTDGKLFPSD GFRDCKKGDP KHGKRKRGRP RKLSKEYWDC LEGKKSKHAP RGTHLWEFIR; Positive Samples: Mouse skin, Mouse lung, SGC-7901, HT-29, SW480; Cellular Location: Cytoplasm, Nucleus

Target Information

ESE-1 (Epithelium-Specific Ets) is a novel, highly tissue-restricted member of the ets transcription factor/oncogene family, which has features distinct from those of any other ets-related factor. ESE-1 binds with high affinity to and transactivates the ets binding site in the promoter of the keratinocyte terminal differentiation marker gene, SPRR2A. ESE-1 is a critical regulator of epithelial cell differentiation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

KDM6A Loss Triggers an Epigenetic Switch That Disrupts Urothelial Differentiation and Drives Cell Proliferation in Bladder Cancer.

Cancer research

Qiu H,Makarov V,Bolzenius JK,Halstead A,Parker Y,Wang A,Iyer GV,Wise H,Kim D,Thayaparan V,Lindner DJ,Haber GP,Ting AH,Ren B,Chan TA,Arora V,Solit DB,Lee BH

PA5-89261 was used in Western Blot to establish that KDM6A loss engenders an epigenetic state that drives tumor growth in an ATF3-dependent manner, creating a potentially targetable molecular vulnerability.

Wed Mar 15 00:00:00 UTC 2023

Peroxynitrite in the tumor microenvironment changes the profile of antigens allowing escape from cancer immunotherapy.

Cancer cell

Tcyganov EN,Sanseviero E,Marvel D,Beer T,Tang HY,Hembach P,Speicher DW,Zhang Q,Donthireddy LR,Mostafa A,Tsyganova S,Pisarev V,Laufer T,Ignatov D,Ferrone S,Meyer C,Maby-El Hajjami H,Speiser DE,Altiok S,Antonia S,Xu X,Xu W,Zheng C,Schuchter LM,Amaravadi RK,Mitchell TC,Karakousis GC,Yuan Z,Montaner LJ,Celis E,Gabrilovich DI

PA5-89261 was used in Western Blot to suggest that intratumoral PNT activity should be considered for the design of neoantigen-based therapy and also may be an important immunotherapeutic target.

Mon Oct 10 00:00:00 UTC 2022

Bioinformatics

Protein Aliases: E74-like factor 3; E74-like factor 3 (ets domain transcription factor); E74-like factor 3 (ets domain transcription factor, epithelial-specific ); E74-like factor 3 (ETS domain transcription factor, serine box, epithelial-specific); ELF3; Epithelial-restricted with serine box; Epithelium-restricted Ets protein ESX; Epithelium-specific Ets transcription factor 1; ESE-1; ets domain transcription factor, serine box (epithelial-specific); ETS-related transcription factor Elf-3; RP11-510N19.1

View more View less

Gene Aliases: ELF3; EPR-1; ERT; ESE-1; ESX; JEN

View more View less

UniProt ID: (Human) P78545, (Mouse) Q3UPW2

View more View less

Entrez Gene ID: (Human) 1999, (Mouse) 13710

View more View less

Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription factor activity, sequence-specific DNA binding transcription coactivator activity protein binding DNA binding sequence-specific DNA binding

Process(es)

blastocyst development transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter inflammatory response multicellular organism development anatomical structure morphogenesis embryo development cell differentiation extracellular matrix organization negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter mammary gland involution regulation of transcription from RNA polymerase II promoter epidermis development epithelial cell differentiation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

ELF3 Polyclonal Antibody

ELF3 Antibody (PA5-89261) in WB

Product Details

PA5-89261

Western Blot (WB)

ELISA (ELISA)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!