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EMAP II Antibody (PA5-30306) in ICC/IF
EMAP II Polyclonal Antibody detects SCYE1 protein at Cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SCYE1 protein stained by EMAP II Polyclonal Antibody (Product # PA5-30306) diluted at 1:500. Blue: Hoechst 33343 staining.
Product Details
PA5-30306
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Fruit fly,
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human AIMP1. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2547780
Product Specific Information
Recommended positive controls: HeLa, Jurkat, NIH-3T3, JC, BCL-1.
Predicted reactivity: Mouse (96%), Rat (97%), Zebrafish (83%), Xenopus laevis (86%), Pig (94%), Bovine (94%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The protein encoded by this gene is a cytokine that is specifically induced by apoptosis, and it is involved in the control of angiogenesis, inflammation, and wound healing. The release of this cytokine renders the tumor-associated vasculature sensitive to tumor necrosis factor. The precursor protein is identical to the p43 subunit, which is associated with the multi-tRNA synthetase complex, and it modulates aminoacylation activity of tRNA synthetase in normal cells. This protein is also involved in the stimulation of inflammatory responses after proteolytic cleavage in tumor cells. Multiple transcript variants encoding different isoforms have been found for this gene. A pseudogene has been identified on chromosome 20.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Aminoacyl tRNA synthase complex-interacting multifunctional protein 1; aminoacyl tRNA synthetase complex-interacting multifunctional protein 1; ARS inducible multifunctional protein 1; ARS-interacting multifunctional protein 1; CG8235; CG8235-PA; endothelial monocyte activating polypeptide 2; endothelial monocyte-activating polypeptide 2; endothelial-monocyte activating polypeptide II; mca1; Multisynthase complex auxiliary component p43; multisynthetase complex auxiliary component p43; small inducible cytokine subfamily E, member 1; small inducible cytokine subfamily E, member 1 (endothelial monocyte-activating)
Gene Aliases: 9830137A06Rik; AIMP1; AIMP1/p43; Dmel\CG8235; Dmel_CG8235; EMAP2; EMAPII; HLD3; p43; SCYE1
UniProt ID: (Human) Q12904
Entrez Gene ID: (Human) 9255, (Mouse) 13722, (Fruit fly) 35892
angiogenesis
negative regulation of endothelial cell proliferation
carbohydrate metabolic process
glucose metabolic process
translation
apoptotic process
inflammatory response
cell adhesion
cell-cell signaling
leukocyte migration
tRNA aminoacylation for protein translation
chemotaxis
signal transduction
response to wounding
defense response to virus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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