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EN2 Antibody (PA5-18474) in ICC/IF
Immunocytochemistry analysis of EN2 using EN2 Polyclonal Antibody (Product # PA5-18474) in paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
Product Details
PA5-18474
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide sequence (NHSTTAKEGKSDSE) corresponding to the C-terminus amino acids of EN2 (aa 320-333).
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10980491
Product Specific Information
This antibody is predicted to react with canine, human and mouse based on sequence homology.
This antibody is tested in Peptide ELISA: antibody detection limit dilution 4,000.
Target Information
Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AUTS1; engrailed homolog 2; engrailed-2; Homeobox protein en-2; Homeobox protein engrailed-2; hu-En-2; mo-En-2
Gene Aliases: BB131122; En-2; EN2
UniProt ID: (Human) P19622, (Mouse) P09066
Entrez Gene ID: (Human) 2020, (Mouse) 13799
multicellular organism development
midbrain development
hindbrain development
negative regulation of neuron apoptotic process
positive regulation of transcription from RNA polymerase II promoter
neuron development
embryonic brain development
regulation of transcription, DNA-templated
neuron differentiation
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