Invitrogen

ENO1 Polyclonal Antibody

ENO1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 12

ENO1 Antibody (PA5-21387) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of ENO1 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: ENO1 Polyclonal Antibody (Product # PA5-21387) diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.

ENO1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ENO1 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

Product Image Gallery

ENO1 Antibody — Figure 5 Validation of expression profiling of proteins by Western blotting. Four of the identified proteins, PRDX1 ( A ), GLRX3 ( B ), ENO1 ( C ), and CASP1 ( D ), responding to AGE and/or FruArg treatment in LPS-stimulated BV-2 cells were validated using Western blotting. Protein intensities were quantified by Image J software, normalized to beta-actin, and expressed as percentage of untreated controls. Data are means +- SEM (n>=5); #, P<0.05, untreated vs. LPS; *, P<0.05, LPS vs. LPS+AGE; **, P<0.05, LPS vs. LPS+FruArg. {TM}

Product Details

PA5-21387

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:20,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

Dot blot (DB)

Assay-dependent

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human ENO1. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 10% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_11154109

Product Specific Information

Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji, mouse brain.

Predicted reactivity: Mouse (94%), Rat (93%), Xenopus laevis (90%), Chicken (93%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (93%), Guinea pig (95%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Enolase (2-phosphogly-cerate hydrolyase or phosphopyruvate hydrates) is a glycolytic enzyme that catalyzes the dehydration and conversion of 2-phosphoglycerate to phosphoenolpyruvate. It comprises three distinct subunits, alpha, beta and gamma. Non Neuronal Enolase(ENO1) is an isoform of mammalian enolase and is composed of 2 alpha subunits. The gene for ENO1 also encodes a shorter monomeric structural lens protein, tau-crystallin. Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.Used as a diagnostic marker for many tumors and, in the heterodimeric form, alpha/gamma, as a marker for hypoxic brain injury after cardiac arrest. Also marker for endometriosis. Antibodies against alpha-enolase are present in sera from patients with cancer-associated retinopathy syndrome (CAR), a progressive blinding disease which occurs in the presence of systemic tumor growth, primarily small-cell carcinoma of the lung and other malignancies. Is identified as an autoantigen in Hashimoto encephalopathy (HE) a rare autoimmune disease associated with Hashimoto thyroiditis (HT). HT is a disorder in which destructive processes overcome the potential capacity of thyroid replacement leading to hypothyroidism.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Quantitative Proteomic Analysis of Differentially Expressed Protein Profiles Involved in Pancreatic Ductal Adenocarcinoma.

Pancreas

Kuo KK,Kuo CJ,Chiu CY,Liang SS,Huang CH,Chi SW,Tsai KB,Chen CY,Hsi E,Cheng KH,Chiou SH

Published figure using ENO1 polyclonal antibody (Product # PA5-21387) in Immunohistochemistry

Fri Jan 01 00:00:00 UTC 2016

Quantitative Proteomic Analysis of Differentially Expressed Protein Profiles Involved in Pancreatic Ductal Adenocarcinoma.

Pancreas

Kuo KK,Kuo CJ,Chiu CY,Liang SS,Huang CH,Chi SW,Tsai KB,Chen CY,Hsi E,Cheng KH,Chiou SH

Published figure using ENO1 polyclonal antibody (Product # PA5-21387) in Immunohistochemistry

Fri Jan 01 00:00:00 UTC 2016

Proteomic analysis of the effects of aged garlic extract and its FruArg component on lipopolysaccharide-induced neuroinflammatory response in microglial cells.

PloS one

Zhou H,Qu Z,Mossine VV,Nknolise DL,Li J,Chen Z,Cheng J,Greenlief CM,Mawhinney TP,Brown PN,Fritsche KL,Hannink M,Lubahn DB,Sun GY,Gu Z

PA5-21387 was used in western blot to study the effects of aged garlic extract and its FruArg component by proteomic analysis and lipopolysaccharide-induced neuroinflammatory response in microglial cells

Tue Jan 26 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: 2-phospho-D-glycerate hydro-lyase; 2-phospho-D-glycerate hydrolase; alpha enolase; alpha enolase like 1; Alpha-enolase; c-Myc promoter binding protein; C-myc promoter-binding protein; enoa; Enolase 1; enolase 1, (alpha); enolase 1, alpha non-neuron; enolase-alpha; epididymis secretory protein Li 17; MBP-1; MPB-1; MYC promoter-binding protein 1; NNE; Non-neural enolase; Phosphopyruvate hydratase; Plasminogen-binding protein; tau-crystallin

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Gene Aliases: 0610008I15; AL022784; Eno-1; ENO1; ENO1L1; HEL-S-17; MBP-1; MBPB1; MPB1; NNE; PPH

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UniProt ID: (Human) P06733, (Mouse) P17182, (Rat) P04764

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Entrez Gene ID: (Human) 2023, (Mouse) 13806, (Rat) 24333

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Function(s)

magnesium ion binding DNA binding transcription factor activity, sequence-specific DNA binding transcription corepressor activity phosphopyruvate hydratase activity protein binding poly(A) RNA binding protein heterodimerization activity GTPase binding cadherin binding involved in cell-cell adhesion RNA binding protein homodimerization activity

Process(es)

negative regulation of transcription from RNA polymerase II promoter in utero embryonic development gluconeogenesis transcription, DNA-templated response to virus negative regulation of cell growth negative regulation of transcription, DNA-templated positive regulation of binding canonical glycolysis cell-cell adhesion cellular response to acid chemical glycolytic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ENO1 Polyclonal Antibody

ENO1 Antibody (PA5-21387) in ICC/IF

Product Details

PA5-21387

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Dot blot (DB)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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