Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
ENO1 Recombinant Rabbit Monoclonal Antibody (JM63-53)
Promotion
View available promotion(s)
Promo code: {{promo.promoCode}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 6
ENO1 Antibody (MA5-32756) in WB
Western blot was performed using anti-ENO1 Monoclonal Antibody (Product # MA5-32756) on whole cell extracts (30 µg lysate) of THP-1 (Lane1), A549 (Lane 2), U-87 MG (Lane 3), Mouse heart (Lane 4), Mouse skeletal muscle (Lane 5) and Rat skeletal muscle (Lane 6) and 47 kDa band corresponding to ENO1 protein was observed across all the samples tested except in Mouse Heart. Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the ... View More
Product Details
MA5-32756
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat,
Zebrafish
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JM63-53
Immunogen
Synthetic peptide within Human ENO1 aa 1-50
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2810033
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
Enolase (2-phosphogly-cerate hydrolyase or phosphopyruvate hydrates) is a glycolytic enzyme that catalyzes the dehydration and conversion of 2-phosphoglycerate to phosphoenolpyruvate. It comprises three distinct subunits, alpha, beta and gamma. Non Neuronal Enolase(ENO1) is an isoform of mammalian enolase and is composed of 2 alpha subunits. The gene for ENO1 also encodes a shorter monomeric structural lens protein, tau-crystallin. Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.Used as a diagnostic marker for many tumors and, in the heterodimeric form, alpha/gamma, as a marker for hypoxic brain injury after cardiac arrest. Also marker for endometriosis. Antibodies against alpha-enolase are present in sera from patients with cancer-associated retinopathy syndrome (CAR), a progressive blinding disease which occurs in the presence of systemic tumor growth, primarily small-cell carcinoma of the lung and other malignancies. Is identified as an autoantigen in Hashimoto encephalopathy (HE) a rare autoimmune disease associated with Hashimoto thyroiditis (HT). HT is a disorder in which destructive processes overcome the potential capacity of thyroid replacement leading to hypothyroidism.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: 2-phospho-D-glycerate hydro-lyase; 2-phospho-D-glycerate hydrolase; alpha enolase; alpha enolase like 1; Alpha-enolase; c-Myc promoter binding protein; C-myc promoter-binding protein; enoa; Enolase 1; enolase 1, (alpha); enolase 1, alpha non-neuron; enolase-alpha; epididymis secretory protein Li 17; MBP-1; MPB-1; MYC promoter-binding protein 1; NNE; Non-neural enolase; Phosphopyruvate hydratase; Plasminogen-binding protein; tau-crystallin
Gene Aliases: 0610008I15; AL022784; Eno-1; ENO1; ENO1L1; HEL-S-17; MBP-1; MBPB1; MPB1; NNE; PPH
UniProt ID: (Human) P06733, (Mouse) P17182, (Rat) P04764
Entrez Gene ID: (Human) 2023, (Mouse) 13806, (Rat) 24333
magnesium ion binding
DNA binding
transcription factor activity, sequence-specific DNA binding
transcription corepressor activity
phosphopyruvate hydratase activity
protein binding
poly(A) RNA binding
protein heterodimerization activity
GTPase binding
cadherin binding involved in cell-cell adhesion
RNA binding
protein homodimerization activity
negative regulation of transcription from RNA polymerase II promoter
in utero embryonic development
gluconeogenesis
transcription, DNA-templated
response to virus
negative regulation of cell growth
negative regulation of transcription, DNA-templated
positive regulation of binding
canonical glycolysis
cell-cell adhesion
cellular response to acid chemical
glycolytic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support